LOCUS       sequence-12331-e       11869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 11869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPGAL4.2Uw-2
FEATURES             Location/Qualifiers
     source          1..11869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2012..4657
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     intron          4746..4811
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4941..4961
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5233..5354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5270..5289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5324..5343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6391..7005,7076..7207,7411..7726,7788..8442,
                     8517..8790,9192..9263))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9715..9733
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9958..10027
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(10114..10702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10194..10213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10873..11664,11665..11733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11496..11515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11734..11838)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accgctagca gatccgggat
     1981 cccgccaagc ttgaagcaag cctcaatcaa aatgaagctg ctgagtagta ttgaacaagc
     2041 gtgtgacata tgccgactta agaagctgaa gtgcagcaag gaaaagccga agtgcgccaa
     2101 atgtcttaag aacaattggg agtgccggta ctcgccaaag acgaagcgaa gccctttgac
     2161 acgcgctcat ttgactgaag tggagagtcg cctggagcgc cttgagcagt tgttcctcct
     2221 catttttccc cgtgaagacc tcgacatgat actgaagatg gatagtctcc aagatattaa
     2281 ggccctgctc actggcctgt ttgttcagga taacgtcaac aaggatgccg tgacggaccg
     2341 ccttgcctcg gtggagacag atatgccact taccttgcgg cagcatcgca tctcggctac
     2401 cagcagtagt gaagaaagtt ccaataaggg tcaacggcaa ctgacggtat cgatcgacag
     2461 tgcggcccac cacgacaaca gcaccattcc actggacttc atgcctcgtg acgcactgca
     2521 tggatttgac tggagcgagg aggatgatat gagcgacgga ctcccattcc tgaagaccga
     2581 ccccaataac aatggtttct ttggcgacgg cagcctgctg tgtatcctgc gaagtattgg
     2641 ttttaagccg gaaaattaca cgaacagtaa tgtcaatcgt cttcccacaa tgatcaccga
     2701 ccgttacacg ctggccagtc gcagtactac aagccgcctg ctgcaaagct atcttaacaa
     2761 cttccacccc tactgcccca tagtgcatag tcctactctc atgatgcttt acaacaatca
     2821 gattgagata gcatcgaagg accagtggca gatcctcttc aactgcattc tcgccatcgg
     2881 cgcttggtgt attgaaggcg agagcacaga tatcgacgtg ttttactatc aaaacgccaa
     2941 gtcgcacctc acctcgaagg tcttcgaaag tggaagcatt atcctcgtga cagccctcca
     3001 tctgctgagt cgttataccc agtggcgtca gaaaactaat acatcctaca actttcattc
     3061 gttcagcatt cggatggcga ttagcctggg acttaatcgt gacctcccaa gtagcttcag
     3121 cgattcgtcg atcctggaac agcgccggag gatttggtgg agtgtgtaca gctgggagat
     3181 ccagctgtcc ctcttgtacg gacgcagcat tcagctgagt cagaacacga tttccttccc
     3241 gtccagcgtg gacgacgttc aacggacaac tacgggcccg accatctacc acggtatcat
     3301 tgagacggcc cgcttgcttc aagtgtttac gaagatttat gagctggata agacagtgac
     3361 cgcggagaag agtccgatct gcgccaagaa atgcctgatg atctgcaatg agatagagga
     3421 ggtgagccgc caggcaccca agttcctgca gatggacata agcactacgg cgctgactaa
     3481 tctcctcaaa gaacacccct ggctgagttt cacccgcttc gagctgaagt ggaaacagct
     3541 gagtctgatt atatacgttt tgcgggactt tttcacaaat tttacgcaga agaagtcgca
     3601 gcttgagcaa gatcagaacg accatcagtc ctacgaggta aagaggtgca gcataatgct
     3661 ctccgacgca gcccagcgca ccgtgatgtc ggtgtcgtcc tatatggaca accataatgt
     3721 gaccccttat ttcgcctgga attgctccta ctatttgttc aacgccgtgt tggtcccaat
     3781 caagactctt ctgagcaact ccaaatcgaa cgccgagaat aacgagacag cacagctctt
     3841 gcagcaaatt aacaccgtgt tgatgctgtt gaagaaactc gctacattca agattcagac
     3901 ttgcgaaaag tacatccagg tgttggaaga ggtgtgcgcc ccattccttc tttcgcaatg
     3961 cgcaattccc ctcccgcaca taagttacaa taattccaac ggctccgcta tcaagaatat
     4021 cgtcggctcc gctacaatcg cgcagtatcc cacgctgcct gaggaaaatg ttaacaacat
     4081 atcggtgaag tatgtaagtc caggcagcgt cggcccgagc cctgtgccgc tgaaaagcgg
     4141 agcctccttc agcgatctgg tcaagctgct cagcaaccgc ccaccatccc gcaactcccc
     4201 tgtcactata ccccgatcga ccccgtcgca tcgtagcgtt accccattcc tgggacaaca
     4261 gcagcaactg cagagtctgg tgccacttac gccctcggca ttgtttggcg gagccaactt
     4321 caaccagagt ggtaacattg cggacagctc cttgagcttc acgtttacca acagctccaa
     4381 cggtccaaac ttgatcacga ctcagacaaa ctcccaggca ttgtcccagc ccattgccag
     4441 ctcgaatgtg catgataact ttatgaataa tgaaattact gcctccaaga ttgacgacgg
     4501 caacaacagc aagccccttt ccccaggctg gactgaccag acggcctaca atgctttcgg
     4561 tatcaccact ggcatgttca acaccacaac gatggacgac gtatataact atctttttga
     4621 tgatgaggat actccaccga acccaaagaa ggagtagaag cttcctaggg atctttgtga
     4681 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     4741 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     4801 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     4861 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     4921 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     4981 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     5041 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     5101 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     5161 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     5221 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5281 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5341 atcaatgtat cttatcatgt ctaccggtac tagttctaga gcggccgctg gccacgggtg
     5401 cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
     5461 tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
     5521 gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
     5581 cggaagtcag cgccctgcac cattatgttc cggaggcgcg ccctagttcc agtgaaatcc
     5641 aagcattttc taaattaaat gtattcttat tattatagtt gttatttttg atatatataa
     5701 acaacactat tatgcccacc atttttttga gatgcatcta cacaaggaac aaacactgga
     5761 tgtcactttc agttcaaatt gtaacgctaa tcactccgaa caggtcacaa aaaattacct
     5821 taaaaagtca taatattaaa ttagaataaa tatagctgtg agggaaatat atacaaatat
     5881 attggagcaa ataaattgta catacaaata tttattacta atttctattg agacgaaatg
     5941 aaccactcgg aaccatttga gcgaaccgaa tcgcgcggaa ctaacgacag tcgctccaag
     6001 gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac
     6061 tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt
     6121 atataaaaaa tatatcccgg tgttttatgt agcgataaac gagtttttga tgtaaggtat
     6181 gcaggtgtgt aagtcttttg gttagaagac aaatccaaag tctacttgtg gggatgttcg
     6241 aaggggaaat acttgtattc tataggtcat atcttgtttt tattggcaca aatataatta
     6301 cattagcttt ttgagggggc aataaacagt aaacacgatg gtaataatgg taaaaaaaaa
     6361 aaacaagcag ttatttcgga tatatgtcgg ctactccttg cgtcgggccc gaagtcttag
     6421 agccagatat gcgagcaccc ggaagctcac gatgagaatg gccagaccca cgtagtccag
     6481 cggcagatcg gcggcggaga agttaagcgt ctccaggatg accttgcccg aactggggca
     6541 cgtggtgttc gacgatgtgc agctaatttc gcccggctcc acgtccgccc attggttaat
     6601 cagcagaccc tcgttggcgt aacggaacca tgagaggtac gacaaccatt tgaggtatac
     6661 tggcaccgag cccgagttca agaagaagcc gccaaagagc aggaatggta tgataaccgg
     6721 cggacccaca gacagcgcca tcgaggtcga ggagctggcg caggatatta gatatccgaa
     6781 ggacgttgac acattggcca ccagagtgac cagcgccagg cagttgaaga agtgcagcac
     6841 tccggcccgc agtccgatca tcggataggc aatcgccgtg aagaccagtg gcactgtgag
     6901 aaaaagcggc aattcggcaa tcgttttgcc cagaaagtat gtgtcacagc gataaagtcg
     6961 acttcgggcc tccctcataa aaactggcag ctctgaggtg aacacctaaa tcgaatcgat
     7021 tcattagaaa gttagtaaat tattgaaatg caaatgtatt ctaaacatga cttacattta
     7081 tcgtggcaaa gacgttttga aaggtcatgt tggtcaggaa gaggaagatg gctccgttga
     7141 tattcatcac acccacttgc gtgagttgtt ggcccaaaaa gatgaggcca atcaagatgg
     7201 caaccatctg caaattaaaa tgttactcgc atctcattaa tattcgcgag ttaaatgaaa
     7261 tttatttatc ttctgcaaaa ctataaacta tacatctcat tgaaaaaaac taagaagggt
     7321 gtggaatcag gcaattctat ctaaaatcta gcgaatttgt ttccaagaat tgtaagcgtt
     7381 atatcatttg tttccactgg aaccactcac cgttgtctga ataagtcgca cttttacgag
     7441 gagtggttcc ttgagcaccg acagccagga tcgccacagg accgcccgga actgcatgaa
     7501 ccaggtggcc ttgtaggtgt acccattctc cggctgctcc agtggcttct ccagattttt
     7561 ggtggccaac aactgctcca tatcccgggc tactttgcta atggcaaaat tgtcgcatat
     7621 cttggcgatc cgatcacggg actcgatctc ccgtccgggc acaacggcca acacctgtac
     7681 gtaaaagtcc gccggattgt agttggtagg acactgggca cccacgctgg ataggagttg
     7741 agatgttatg taatactaga tacccttaat aaacacatcg aactcactag gaaaagaagt
     7801 cgacggcttc gctgggagtg cccaagaaag ctaccctgcc ctcggccatc agaaggatct
     7861 tgtcaaagag ctcaaacagc tcggaagacg gctgatgaat ggtcaggatg acggtcttgc
     7921 ccttctgcga cagcttcttc agcacctgga cgacgctgtg ggcggtaaag gagtccagtc
     7981 cggaggtggg ctcatcgcag atcagaagcg gcggatcggt tagagcctcg gaggcgaatg
     8041 ccagacgctt cctttctccg ccggacagac ctttcaccct gccgggcaca ccgatgatcg
     8101 tgtgctgaca tttgctgagc gaaagctcct ggatcacctg atccacgcgg gccactcgct
     8161 gccgataggt cagatgtcgt ggcatccgca ccatggcttg gaaaatcagg tgttccctgg
     8221 ccgttaggga gccgataaag aggtcatcct gctggacata ggcgcacctg gcctgcatct
     8281 ccttggcgtc cacaggttgg ccattgagca gtcgcatccc ggatggcgat acttggatgc
     8341 cctgcggcga tcgaaaggca agggcattca gcagggtcgt ctttccggca ccggaactgc
     8401 ccatcacggc caaaagttcg cccggatagg ccacgccgca aactgagttt caaattggta
     8461 attggaccct ttattaagat ttcacacaga tcagccgact gcgaatagaa actcaccgtt
     8521 cttgagcaaa tgtttcctgg gcgccggtat gtgtcgctcg ttgcagaata gtccgcgtgt
     8581 ccggttgacc agctgccgcc atccggagcc cggctgattg accgccccaa agatgtccat
     8641 attgtgccag gcataggtga ggttctcggc tagttggccg ctccctgaac cggagtcctc
     8701 cggcggactg ggtggccgga gcgtgccgta gtttttggcc tgcccgaagc cctggttaat
     8761 gcagctctgc gaagccgctc cgctgtcacc ctgcaatgat aggggatctc aaatatcaac
     8821 tactagcgtt atgctcatct aaccccgaac aaaaagtacc ccgaagtatc ctacgaagta
     8881 ggtttatact tttatttatt ttttgtgcat ctaggatcag cttaaaatat ctggttgtta
     8941 tattttttgt aaaaaagaat atagtcgaaa atgaatgcct ttagatgtct tgatcatgat
     9001 atgatctcaa aaattgtctt atatagcgag aacagctacc agaataatct gtttcgtgtc
     9061 actatttgtt tgtgcaattg cggtttggga tttttgtggg tcgcagttct cacgccgcat
     9121 acaatttgat gttgcaatcg cagttcctat agatcaagtg aacttaagat gtatgcacat
     9181 gtactactca cattgttcag atgctcggca gatgggtgtt tgctgcctcc gcgaattaat
     9241 agctcctgat cctcttggcc cattgccggg atttttcaca ctttcccctg cttacccacc
     9301 caaaaccaat caccacccca atcactcaaa aaacaaacaa aaataagaag cgagaggagt
     9361 tttggcacag cactttgtgt ttaattgatg gcgtaaaccg cttggagctt cgtcacgaaa
     9421 ccgctgacaa aatgcaactg aaggcggaca ttgacgctac gtaacgctac aaacggtggc
     9481 gaaagagata gcggacgcag cggcgaaaga gacggcgata tttctgtgga cagagaagga
     9541 ggcaaacagc gctgactttg agtggaatgt cattttgagt gagaggtaat cgaaagaacc
     9601 tggtacatca aatacccttg gatcgaagta aatttaaaac tgatcagata agttcaatga
     9661 tatccagtgc agtaaaaaaa aaaaatgttt tttttatcta ctttccgcaa aaatgggttt
     9721 tattaactta catacatact aggcgcgccc atatgttcgg cttgtcgaca tgcccgccgt
     9781 gaccgtcgag aacccgctga cgctgccccg cgtatccgca cccgccgacg ccgtcgcacg
     9841 tcccgtgctc accgtgacca ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg
     9901 ccgcgcgttc gccgggatca actaccgcca cctcgacccg ttcatcatga tggaccagat
     9961 gggtgaggtg gagtacgcgc ccggggagcc caagggcacg ccctggcacc cgcaccgcgg
    10021 cttcgagacc gtgacctaca tcgtcgacgg taacatgtga gcaaaaggcc agcaaaaggc
    10081 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10141 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10201 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10261 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10321 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10381 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10441 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10501 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    10561 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10621 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11461 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    11761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
