LOCUS       sequence-123310-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nuclease competent SP-Cas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: hCas9-VPR
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..113)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagattatcg aacttccgtt gtgtgatcag tttggcgttc agcagctgcc gccaataatt
       61 tttcattttc ttgacaactt cttctgaggg gacgttatca ctcttccctc tatttttatc
      121 ggatcttgtc aacactttat tatcaataga atcatctttg agaaaagact ggggcacgat
      181 atgatccacg tcgtagtcgg agagccgatt gatgtccagt tcctgatcca cgtacatgtc
      241 cctgccgttc tgcaggtagt acaggtagag cttctcattc tgaagctggg tgttttcaac
      301 tgggtgttcc ttaaggattt gggaccccag ttcttttata ccctcttcaa tcctcttcat
      361 cctttcccta ctgttcttct gtcccttctg ggtagtttgg ttctctcggg ccatctcgat
      421 aacgatattc tcgggcttat gccttcccat tactttgacg agttcatcca cgaccttaac
      481 ggtctgcagt attccctttt tgatagctgg gctacctgca agattagcga tgtgctcgtg
      541 aagactgtcc ccctggccag aaacttgtgc tttctggatg tcctccttaa aggtgagaga
      601 gtcatcatgg atcaactgca tgaagttccg gttggcaaat ccatcggact taagaaaatc
      661 caggattgtc tttccactct gcttgtctcg gatcccattg atcagttttc ttgacagccg
      721 cccccatcct gtatatcggc gcctcttgag ctgtttcatg actttgtcgt cgaagagatg
      781 agcgtaagtt ttcaagcgtt cttcaatcat ctccctatct tcaaacaacg taagggtgag
      841 gacaatgtcc
//
