LOCUS       sequence-123313-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral plasmid with multiple cloning site and puromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: MSCV Puro
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     443..462
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(531..550)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg
       61 gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg
      121 cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat
      181 gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac
      241 cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt
      301 gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg
      361 gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc
      421 gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggtaagct tgggctgcag
      481 gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc
      541 gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc
      601 ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa
      661 agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc
      721 atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc
      781 tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca
      841 ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg
      901 ccacttgtgt agcgccaagt g
//
