LOCUS       sequence-123319-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral backbone for use with GMAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: LV 1-5
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    136..253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(406..425)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     753..774
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(924..944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
       61 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
      121 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      181 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      241 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt tagtaccggg
      301 cccgggtcga ctagtgatca gtgtgaggga gtgtaaagct ggtttaaact gtacagctcg
      361 tccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      421 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      481 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      541 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggc gaacttcaag
      601 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      661 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
      721 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      781 gccgggatca ctctcggcat ggacgagctg tactgtacaa acgttgttgt ttggggttga
      841 attactcttc tagaccgcgg cgcgcccgat aatcaacctc tggattacaa aatttgtgaa
      901 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
      961 atgcctttgt atcatgctat tgcttcccgt atggctttca ttt
//
