LOCUS       sequence-123320-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral backbone for use with GMAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: LV 1-5
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     71..87
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     72..88
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    91..679
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             197..208
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     606..626
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(776..797)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc
       61 taggtctcga tcgaggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac
      121 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
      181 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
      241 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
      301 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
      361 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
      421 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
      481 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
      541 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
      601 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
      661 tgtaatccag aggttgatta tcgggcgcgc cgcggtctag aagagtaatt caaccccaaa
      721 caacaacgtt tgtacagtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      781 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      841 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      901 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttcg
      961 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
     1021 actccagctt gtgccccagg atgttgccg
//
