LOCUS       sequence-123324-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing mCherry for generation of GMAP-compatible 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO mCherry
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     244..266
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     258..275
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     259..275
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     282..301
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        282..300
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(707..726)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcagacgtgc actggccagg gggatcacca tccgtcgccc cggcgtgtca ataatatcac
       61 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
      121 gccgtacgta taggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      181 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      241 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
      301 gcgaattggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
      361 attccagcac actggcggcc gttactagtg gatccagagt aattcaaccc caaacaacaa
      421 cgttttcaag acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
      481 tcgtattgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
      541 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
      601 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
      661 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
      721 acggggccgt cggaggggaa gttggttccc cgcagcttaa ctttgtagat gaactcgccg
      781 tcttgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
      841 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
      901 tgcttcacgt aggccttgga gccatacata aattgagggg acaggatgtc ccaggcgaag
      961 ggcagggggc cacccttggt aaccttcagc ttggctgttt gggtgccctc gtaggggcgg
     1021 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgaact
     1081 ttgaagcgca taaattcctt gatgatgg
//
