LOCUS       sequence-123325-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vimentin-targeted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Vimentin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc accatgtcca ccaggtccgt gtcctcgtcc tcctaccgca ggatgttcgg
      121 cggcccgggc accgcgagcc ggccgagctc cagccggagc tacgtgacta cgtccacccg
      181 cacctacagc ctgggcagcg cgctgcgccc cagcaccagc cgcagcctct acgcctcgtc
      241 cccgggcggc gtgtatgcca cgcgctcctc tgccgtgcgc ctgcggagca gcgtgcccgg
      301 ggtgcggctc ctgcaggact cggtggactt ctcgctggcc gacgccatca acaccgagtt
      361 caagaacacc cgcaccaacg agaaggtgga gctgcaggag ctgaatgacc gcttcgccaa
      421 ctacatcgac aaggtgcgct tcctggagca gcagaataag atcctgctgg ccgagctcga
      481 gcagctcaag ggccaaggca agtcgcgcct gggggacctc tacgaggagg agatgcggga
      541 gctgcgccgg caggtggacc agctaaccaa cgacaaagcc cgcgtcgagg tggagcgcga
      601 caacctggcc gaggacatca tgcgcctccg ggagaaattg caggaggaga tgcttcagag
      661 agaggaagcc gaaaacaccc tgcaatcttt cagacaggat gttgacaatg cgtctctggc
      721 acgtcttgac cttgaacgca aagtggaatc tttgcaagaa gagattgcct ttttgaagaa
      781 actccacgaa gaggaaatcc aggagctgca ggctcagatt caggaacagc atgtccaaat
      841 cgatgtggat gtttccaagc ctgacctcac ggctgccctg cgtgacgta
//
