LOCUS       sequence-123326-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vimentin-targeted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Vimentin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..742)
                     /codon_start=1
                     /product="fast-switching photoswitchable fluorescent 
                     protein useful for fluorescence nanoscopy (Tiwari et al., 
                     2015)"
                     /label=Kohinoor
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSL
                     EGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSGLPRQ
                     AK"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattctta cttggcctgc ctcggcagcc cagaatgcgc ttcggcatgc tcatgcagat
      121 taacattact gtaatctctg tcgtggcttt taatctcaat gtggtggacc acatcgtgat
      181 agtctggcaa ctggacaacc ttcttagctt tataagtagt tttggagtca catcggtagt
      241 ggccacctcc ttcaagcgac agagcgtagt tgccatcgct cttcagcact ccatcacgca
      301 catacaattt ctcagtggat ggctcccatt tcacagtcct cttctgcata actggaccat
      361 tggcaggaaa gttcacacca tcaaatcgaa tttcatagat ataacagtca ccatccaggg
      421 ttatgtcgtt tgtcgcgata caaatgcccc cgtcttcgta aatcatgctt cgttcccaag
      481 agtagccctc aggaaacgac tgcttgaaat aatctactat attttctggg tatttggcga
      541 ataccctgtt gccgtaacag aaggccattg tcaagatgtc ataggcgaaa ggcagaggtc
      601 cgccttcttt gactttaagg tccatactct gttttccctc gaaaggcttc ccaaggccaa
      661 ctccttcaat cgcgaacggg tgtccattta cagcgccttc catacgcagc ttgatcttca
      721 tgtctggttt aatcacactc atggatccac cgctgccacc gccaccgctg ccaccgccac
      781 cgctgccggt accttcaagg tcatcgtgat gctgagaagt ttcgttgata acctgtccat
      841 ctctagtttc
//
