LOCUS       sequence-123329-        3415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal 
            promoter in a 5' entry vector for gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Tamplin OJ, Durand EM, Carr LA, Childs SJ, Hagedorn EJ, Li P, 
            Yzaguirre AD, Speck NA, Zon LI
  TITLE     Hematopoietic stem cell arrival triggers dynamic remodeling of the 
            perivascular niche.
  JOURNAL   Cell. 2015 Jan 15;160(1-2):241-52. doi: 10.1016/j.cell.2014.12.032.
  PUBMED    25594182
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2015-01-15"; volume: "160(1-2):241-52. doi"; pages: " 
            10.1016/j.cell.2014.12.032"
COMMENT     Sequence Label: p5E-Runx1+23
FEATURES             Location/Qualifiers
     source          1..3415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1442..1460)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    1461..1584
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1657..1675)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1680..1696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1680..1696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1798..1817)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1809..2618
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1876..1895)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2765..3353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3254..3273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgggccccc cctcgaggga tccggggtgg gaggtgtaag ttccaccccc acccttcctg
      781 acacgctcct gaaccctggc cactgcacct gctaggttct cacttctctg ggaagcatct
      841 agaaacagga cctctcaccc acccctcccg gctgggctct agggtggggc cctcactacc
      901 tcttttcttc tcaaagagcc tgggatgctg acagcctcag atggaggcat cctgtttgtc
      961 gaaaaataaa ccggcagttg aagccgggtt gcaagagcga gaaaaccgca ggcctgcgcg
     1021 ccactgataa cgtgggcagc ttgcttttgc agcagttcct agctgcagcg gccctgtgaa
     1081 ggcctgtgtc accgcctccc ttcctgtctc ctcctcacac catccctcca tcgctccttg
     1141 ctggctctac cagccacttg ctggaccctt cagccactgg gaccattgct ttccataaaa
     1201 aactccttag tgcagtgcag agctgatcag agggtagcag ggaaccctga cgcctgatgg
     1261 gttgctgaca cctgagatcc actagtccaa tctgctcaga gaggacagag tgggcaggag
     1321 ccagcattgg gtatataaag ctgagcaggg tcagttgctt cttacgtttg cttctgattc
     1381 tgttgtgttg acttgcaacc tcagaaacag acatcccgcg gtggagctcc agcttttgtt
     1441 ccctttagtg agggttaatt caagtttgta caaaaaagtt gaacgagaaa cgtaaaatga
     1501 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     1561 aacacaacat atgcagtcac tatgaaccaa ctacttagat ggtattagtg acctgtagaa
     1621 ttcgagctct agagctgcag ggcggccgcg atatccccta tagtgagtcg tattacatgg
     1681 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1741 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1801 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1861 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1921 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1981 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2041 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2101 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2161 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2221 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2281 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2341 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2401 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2461 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2521 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2581 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2641 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     2701 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2881 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3121 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     3181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
