LOCUS       sequence-12333-e       10038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPGAL4.2::p65Uw
FEATURES             Location/Qualifiers
     source          1..10038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2008..2448
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2893..3264
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     primer_bind     complement(3533..3549)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3533..3549)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4560..5174,5245..5376,5580..5895,5957..6611,
                     6686..6959,7361..7432))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7884..7902
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8127..8196
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8283..8871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8363..8382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9042..9833,9834..9902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9665..9684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9903..10007)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accgctagca gatccgcccg
     1981 ccaagcttga agcaagcctc aatcaaaatg aagctgctga gtagtattga acaagcgtgt
     2041 gacatatgcc gacttaagaa gctgaagtgc agcaaggaaa agccgaagtg cgccaaatgt
     2101 cttaagaaca attgggagtg ccggtactcg ccaaagacga agcgaagccc tttgacacgc
     2161 gctcatttga ctgaagtgga gagtcgcctg gagcgccttg agcagttgtt cctcctcatt
     2221 tttccccgtg aagacctcga catgatactg aagatggata gtctccaaga tattaaggcc
     2281 ctgctcactg gcctgtttgt tcaggataac gtcaacaagg atgccgtgac ggaccgcctt
     2341 gcctcggtgg agacagatat gccacttacc ttgcggcagc atcgcatctc ggctaccagc
     2401 agtagtgaag aaagttccaa taagggtcaa cggcaactga cggtatcggg atccacgccg
     2461 atggagttcc agtacctgcc cgatacggat gaccgtcacc gtatcgaaga aaagcggaag
     2521 cgaacctatg aaaccttcaa gtccatcatg aaaaagtccc ccttctcggg ccccacggac
     2581 ccgcgccccc cgccccgtcg tattgcggtt ccttcgcgca gcagtgccag cgtccccaaa
     2641 cccgcaccgc agccctaccc gttcacttcc tcccttagca cgattaacta tgatgagttc
     2701 cccacgatgg tgttccccag tggacaaatt tcccaggcat cggcactggc tccggcccca
     2761 ccgcaagtcc tcccccaggc gcccgctccg gcaccggctc ccgcaatggt gagtgctctg
     2821 gcccaggccc ccgctccagt ccccgtgctg gcgcctggac ccccacaggc agttgcccct
     2881 cctgctccga aaccaacgca ggcgggcgaa ggaaccctga gcgaggccct cttgcagctt
     2941 cagttcgatg acgaagactt gggagccctg ctgggtaaca gcacagaccc tgccgtattc
     3001 accgatctcg catccgtgga caacagcgag tttcagcagc tcttgaatca gggaatcccg
     3061 gtcgcacctc ataccacaga gcccatgctg atggaatacc cggaggctat cacgcgactg
     3121 gtgaccggcg cacagcgacc acccgatcca gcccctgccc cactgggtgc cccgggtttg
     3181 cccaatggcc tcctcagcgg cgatgaggat ttctccagca tcgctgatat ggatttctcc
     3241 gctttgctga gccagataag ctcctaagtt taaacaagct tatcgatacc gtcgactaaa
     3301 gccaaataga aaattattca gttcctggct taagttttta aaagtgatat tatttatttg
     3361 gttgtaacca accaaaagaa tgtaaataac taatacataa ttatgttagt tttaagttag
     3421 caacaaattg attttagcta tattagctac ttggttaata aatagaatat atttatttaa
     3481 agataattgc gtttttattg tcagggagtg agtttgctta aaaactcgtt tagatccact
     3541 agttctagag cggccgctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc
     3601 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
     3661 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
     3721 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
     3781 ggaggcgcgc cctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt
     3841 attatagttg ttatttttga tatatataaa caacactatt atgcccacca tttttttgag
     3901 atgcatctac acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat
     3961 cactccgaac aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat
     4021 atagctgtga gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat
     4081 ttattactaa tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat
     4141 cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga
     4201 gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat
     4261 gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta
     4321 gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca
     4381 aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata
     4441 tcttgttttt attggcacaa atataattac attagctttt tgagggggca ataaacagta
     4501 aacacgatgg taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc
     4561 tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg
     4621 atgagaatgg ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc
     4681 tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg
     4741 cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat
     4801 gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg
     4861 ccaaagagca ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag
     4921 gagctggcgc aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc
     4981 agcgccaggc agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggataggca
     5041 atcgccgtga agaccagtgg cactgtgaga aaaagcggca attcggcaat cgttttgccc
     5101 agaaagtatg tgtcacagcg ataaagtcga cttcgggcct ccctcataaa aactggcagc
     5161 tctgaggtga acacctaaat cgaatcgatt cattagaaag ttagtaaatt attgaaatgc
     5221 aaatgtattc taaacatgac ttacatttat cgtggcaaag acgttttgaa aggtcatgtt
     5281 ggtcaggaag aggaagatgg ctccgttgat attcatcaca cccacttgcg tgagttgttg
     5341 gcccaaaaag atgaggccaa tcaagatggc aaccatctgc aaattaaaat gttactcgca
     5401 tctcattaat attcgcgagt taaatgaaat ttatttatct tctgcaaaac tataaactat
     5461 acatctcatt gaaaaaaact aagaagggtg tggaatcagg caattctatc taaaatctag
     5521 cgaatttgtt tccaagaatt gtaagcgtta tatcatttgt ttccactgga accactcacc
     5581 gttgtctgaa taagtcgcac ttttacgagg agtggttcct tgagcaccga cagccaggat
     5641 cgccacagga ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta cccattctcc
     5701 ggctgctcca gtggcttctc cagatttttg gtggccaaca actgctccat atcccgggct
     5761 actttgctaa tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc
     5821 cgtccgggca caacggccaa cacctgtacg taaaagtccg ccggattgta gttggtagga
     5881 cactgggcac ccacgctgga taggagttga gatgttatgt aatactagat acccttaata
     5941 aacacatcga actcactagg aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc
     6001 taccctgccc tcggccatca gaaggatctt gtcaaagagc tcaaacagct cggaagacgg
     6061 ctgatgaatg gtcaggatga cggtcttgcc cttctgcgac agcttcttca gcacctggac
     6121 gacgctgtgg gcggtaaagg agtccagtcc ggaggtgggc tcatcgcaga tcagaagcgg
     6181 cggatcggtt agagcctcgg aggcgaatgc cagacgcttc ctttctccgc cggacagacc
     6241 tttcaccctg ccgggcacac cgatgatcgt gtgctgacat ttgctgagcg aaagctcctg
     6301 gatcacctga tccacgcggg ccactcgctg ccgataggtc agatgtcgtg gcatccgcac
     6361 catggcttgg aaaatcaggt gttccctggc cgttagggag ccgataaaga ggtcatcctg
     6421 ctggacatag gcgcacctgg cctgcatctc cttggcgtcc acaggttggc cattgagcag
     6481 tcgcatcccg gatggcgata cttggatgcc ctgcggcgat cgaaaggcaa gggcattcag
     6541 cagggtcgtc tttccggcac cggaactgcc catcacggcc aaaagttcgc ccggataggc
     6601 cacgccgcaa actgagtttc aaattggtaa ttggaccctt tattaagatt tcacacagat
     6661 cagccgactg cgaatagaaa ctcaccgttc ttgagcaaat gtttcctggg cgccggtatg
     6721 tgtcgctcgt tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc
     6781 ggctgattga ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct
     6841 agttggccgc tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag
     6901 tttttggcct gcccgaagcc ctggttaatg cagctctgcg aagccgctcc gctgtcaccc
     6961 tgcaatgata ggggatctca aatatcaact actagcgtta tgctcatcta accccgaaca
     7021 aaaagtaccc cgaagtatcc tacgaagtag gtttatactt ttatttattt tttgtgcatc
     7081 taggatcagc ttaaaatatc tggttgttat attttttgta aaaaagaata tagtcgaaaa
     7141 tgaatgcctt tagatgtctt gatcatgata tgatctcaaa aattgtctta tatagcgaga
     7201 acagctacca gaataatctg tttcgtgtca ctatttgttt gtgcaattgc ggtttgggat
     7261 ttttgtgggt cgcagttctc acgccgcata caatttgatg ttgcaatcgc agttcctata
     7321 gatcaagtga acttaagatg tatgcacatg tactactcac attgttcaga tgctcggcag
     7381 atgggtgttt gctgcctccg cgaattaata gctcctgatc ctcttggccc attgccggga
     7441 tttttcacac tttcccctgc ttacccaccc aaaaccaatc accaccccaa tcactcaaaa
     7501 aacaaacaaa aataagaagc gagaggagtt ttggcacagc actttgtgtt taattgatgg
     7561 cgtaaaccgc ttggagcttc gtcacgaaac cgctgacaaa atgcaactga aggcggacat
     7621 tgacgctacg taacgctaca aacggtggcg aaagagatag cggacgcagc ggcgaaagag
     7681 acggcgatat ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc
     7741 attttgagtg agaggtaatc gaaagaacct ggtacatcaa atacccttgg atcgaagtaa
     7801 atttaaaact gatcagataa gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt
     7861 ttttatctac tttccgcaaa aatgggtttt attaacttac atacatacta ggcgcgccca
     7921 tatgttcggc ttgtcgacat gcccgccgtg accgtcgaga acccgctgac gctgccccgc
     7981 gtatccgcac ccgccgacgc cgtcgcacgt cccgtgctca ccgtgaccac cgcgcccagc
     8041 ggtttcgagg gcgagggctt cccggtgcgc cgcgcgttcg ccgggatcaa ctaccgccac
     8101 ctcgacccgt tcatcatgat ggaccagatg ggtgaggtgg agtacgcgcc cggggagccc
     8161 aagggcacgc cctggcaccc gcaccgcggc ttcgagaccg tgacctacat cgtcgacggt
     8221 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8281 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8341 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8401 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8461 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8521 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8581 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8641 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8701 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8761 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8821 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8881 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8941 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9001 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9061 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9121 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9181 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9241 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9301 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9361 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9421 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9481 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9541 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9601 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9661 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9721 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9781 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9841 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9901 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9961 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10021 aaagtgccac ctgacgtc
//
