LOCUS       sequence-123331-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentivirus expressing an arbitrary shRNA and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pTRE:shRNA A-pGK:rtTA-Cre
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(33..35,36..38,39..752)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           796..917
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           945..959
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 taactcgaac gctagctgtg cgatcgttta ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtctggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      241 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      481 acgtctatat catggccgac aagcagaaga acggcatcaa ggcgaacttc aagatccgcc
      541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt aatgacgtac ggctaagcct cgactaggga
      781 taacagggta attgtttgaa tgaggcttca gtactttaca gaatcgttgc ctgcacatct
      841 tggaaacact tgctgggatt acttcttcag gttaacccaa cagaaggctc gagaaggtat
      901 attgctgttg acagtgagcg cccactacaa gtacatgtgt aatagtgaag ccacagatgt
      961 attac
//
