LOCUS       sequence-123333-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Paxilin-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Paxillin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccaccatg gacgacctcg atgccttact ggcagacctg
      121 gaatccacca cctcgcatat ctctaagcga ccggtgtttc taacagagga aacgccttac
      181 tcctacccaa ctggaaacca tacgtaccag gagattgctg tgccaccgcc ggtgcccccg
      241 ccgccttcca gtgaggccct aaatggcact gtgattgacc ccttagacca gtggcaaccc
      301 agcgtgtcca gatacggcca ccagcaacct cagtcccagt ctccgatata cagctctagt
      361 gccaaaagct ccagtgcctc tgttcctaga gacgggctca gctccccttc tccccgtgcc
      421 agtgaggagg aacacgtgta cagcttccca aacaagcaga agtctgcaga accatctccc
      481 acaatgacca gcacctccct gggcagcaac ctctcggaac tggacaggct tctcctggaa
      541 ctgaatgctg ttcaacataa tccccccagt ggcttctcag cagatgaagt cagcagaagc
      601 ccatcactgc ccaatgtgac cggacctcac tatgtcatcc cagagagcag cagctctgca
      661 ggagggaagg ctgcaccccc tacaaaagaa aaaccaaagc ggaatggtgg gcgtgggatt
      721 gaagatgtgc gtcccagtgt ggagagcctg ctggatgagc tggagagctc tgtgccaagc
//
