LOCUS       sequence-123334-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Paxilin-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Paxillin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..741)
                     /codon_start=1
                     /product="fast-switching photoswitchable fluorescent 
                     protein useful for fluorescence nanoscopy (Tiwari et al., 
                     2015)"
                     /label=Kohinoor
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSL
                     EGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSGLPRQ
                     AK"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcttac ttggcctgcc tcggcagccc agaatgcgct tcggcatgct catgcagatt
      121 aacattactg taatctctgt cgtggctttt aatctcaatg tggtggacca catcgtgata
      181 gtctggcaac tggacaacct tcttagcttt ataagtagtt ttggagtcac atcggtagtg
      241 gccacctcct tcaagcgaca gagcgtagtt gccatcgctc ttcagcactc catcacgcac
      301 atacaatttc tcagtggatg gctcccattt cacagtcctc ttctgcataa ctggaccatt
      361 ggcaggaaag ttcacaccat caaatcgaat ttcatagata taacagtcac catccagggt
      421 tatgtcgttt gtcgcgatac aaatgccccc gtcttcgtaa atcatgcttc gttcccaaga
      481 gtagccctca ggaaacgact gcttgaaata atctactata ttttctgggt atttggcgaa
      541 taccctgttg ccgtaacaga aggccattgt caagatgtca taggcgaaag gcagaggtcc
      601 gccttctttg actttaaggt ccatactctg ttttccctcg aaaggcttcc caaggccaac
      661 tccttcaatc gcgaacgggt gtccatttac agcgccttcc atacgcagct tgatcttcat
      721 gtctggttta atcacactca tggatccacc gctgccaccg ccaccgctgc caccgccacc
      781 gctgccggta ccacagaaga gtttgagaaa gcagttctga cagtagggct tgtcgttctg
      841 ttctttgaag gttcctttgt
//
