LOCUS       sequence-123335-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Actin-targetted Kohinoor for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Tiwari DK, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, 
            Fujita K, Nagai T
  TITLE     A fast- and positively photoswitchable fluorescent protein for 
            ultralow-laser-power RESOLFT nanoscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 
            2015 Apr 20.
  PUBMED    25894946
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3362"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "515-8"
COMMENT     Sequence Label: Kohinoor-Actin/pcDNA3
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ccatggtgag tgtgattaaa
      121 ccagacatga agatcaagct gcgtatggaa ggcgctgtaa atggacaccc gttcgcgatt
      181 gaaggagttg gccttgggaa gcctttcgag ggaaaacaga gtatggacct taaagtcaaa
      241 gaaggcggac ctctgccttt cgcctatgac atcttgacaa tggccttctg ttacggcaac
      301 agggtattcg ccaaataccc agaaaatata gtagattatt tcaagcagtc gtttcctgag
      361 ggctactctt gggaacgaag catgatttac gaagacgggg gcatttgtat cgcgacaaac
      421 gacataaccc tggatggtga ctgttatatc tatgaaattc gatttgatgg tgtgaacttt
      481 cctgccaatg gtccagttat gcagaagagg actgtgaaat gggagccatc cactgagaaa
      541 ttgtatgtgc gtgatggagt gctgaagagc gatggcaact acgctctgtc gcttgaagga
      601 ggtggccact accgatgtga ctccaaaact acttataaag ctaagaaggt tgtccagttg
      661 ccagactatc acgatgtggt ccaccacatt gagattaaaa gccacgacag agattacagt
      721 aatgttaatc tgcatgagca tgccgaagcg
//
