LOCUS       sequence-12334-e       10208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPnlsLexA::p65Uw
FEATURES             Location/Qualifiers
     source          1..10208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1983..2003
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2013..2264,2265..2618)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             3063..3434
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     primer_bind     complement(3703..3719)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3703..3719)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4730..5344,5415..5546,5750..6065,6127..6781,
                     6856..7129,7531..7602))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8054..8072
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8297..8366
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8453..9041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8533..8552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9212..10003,10004..10072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9835..9854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10073..10177)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accgctagca atcaaaatgc
     1981 cacccaagaa gaagcgaaaa gtagaagatc caatgaaggc tctcacggcc cgacaacagg
     2041 aagtttttga tttgatacgg gatcatatat cccaaacggg tatgcctccg acccgcgcag
     2101 agatagcaca gcgactgggc tttcgatcgc ctaacgccgc ggaggagcac ttgaaggcac
     2161 tggcccgcaa gggtgtcatt gaaatcgtgt ccggtgcgag ccgcggaatc cggctgttgc
     2221 aggaggagga agagggcctg ccactggtgg gacgcgtggc cgctggcgag ccgctgctgg
     2281 cccagcaaca catagaggga cactatcagg tggacccctc cttgtttaag ccaaatgctg
     2341 atttcctgtt gcgggtgtcg ggaatgtcca tgaaggacat cggtattatg gatggtgacc
     2401 tcctcgccgt ccataagaca caggatgtca ggaacggcca ggtagtcgtt gccaggatag
     2461 acgatgaggt cactgtgaaa cgtctcaaga agcaaggcaa taaggtcgag ctgctgccgg
     2521 agaatagcga gttcaagccg atcgtggtgg atctgcgaca gcagtccttt actatcgagg
     2581 gcttggccgt gggtgtgatc cgcaacggag attggctggg atccacgccg atggagttcc
     2641 agtacctgcc cgatacggat gaccgtcacc gtatcgaaga aaagcggaag cgaacctatg
     2701 aaaccttcaa gtccatcatg aaaaagtccc ccttctcggg ccccacggac ccgcgccccc
     2761 cgccccgtcg tattgcggtt ccttcgcgca gcagtgccag cgtccccaaa cccgcaccgc
     2821 agccctaccc gttcacttcc tcccttagca cgattaacta tgatgagttc cccacgatgg
     2881 tgttccccag tggacaaatt tcccaggcat cggcactggc tccggcccca ccgcaagtcc
     2941 tcccccaggc gcccgctccg gcaccggctc ccgcaatggt gagtgctctg gcccaggccc
     3001 ccgctccagt ccccgtgctg gcgcctggac ccccacaggc agttgcccct cctgctccga
     3061 aaccaacgca ggcgggcgaa ggaaccctga gcgaggccct cttgcagctt cagttcgatg
     3121 acgaagactt gggagccctg ctgggtaaca gcacagaccc tgccgtattc accgatctcg
     3181 catccgtgga caacagcgag tttcagcagc tcttgaatca gggaatcccg gtcgcacctc
     3241 ataccacaga gcccatgctg atggaatacc cggaggctat cacgcgactg gtgaccggcg
     3301 cacagcgacc acccgatcca gcccctgccc cactgggtgc cccgggtttg cccaatggcc
     3361 tcctcagcgg cgatgaggat ttctccagca tcgctgatat ggatttctcc gctttgctga
     3421 gccagataag ctcctaagtt taaacaagct tatcgatacc gtcgactaaa gccaaataga
     3481 aaattattca gttcctggct taagttttta aaagtgatat tatttatttg gttgtaacca
     3541 accaaaagaa tgtaaataac taatacataa ttatgttagt tttaagttag caacaaattg
     3601 attttagcta tattagctac ttggttaata aatagaatat atttatttaa agataattgc
     3661 gtttttattg tcagggagtg agtttgctta aaaactcgtt tagatccact agttctagag
     3721 cggccgctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
     3781 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
     3841 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
     3901 ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggaggcgcgc
     3961 cctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt attatagttg
     4021 ttatttttga tatatataaa caacactatt atgcccacca tttttttgag atgcatctac
     4081 acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat cactccgaac
     4141 aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat atagctgtga
     4201 gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat ttattactaa
     4261 tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat cgcgcggaac
     4321 taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg
     4381 gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg
     4441 cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg
     4501 agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt
     4561 ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt
     4621 attggcacaa atataattac attagctttt tgagggggca ataaacagta aacacgatgg
     4681 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
     4741 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
     4801 ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga
     4861 ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca
     4921 cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg
     4981 acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca
     5041 ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag gagctggcgc
     5101 aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc agcgccaggc
     5161 agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggataggca atcgccgtga
     5221 agaccagtgg cactgtgaga aaaagcggca attcggcaat cgttttgccc agaaagtatg
     5281 tgtcacagcg ataaagtcga cttcgggcct ccctcataaa aactggcagc tctgaggtga
     5341 acacctaaat cgaatcgatt cattagaaag ttagtaaatt attgaaatgc aaatgtattc
     5401 taaacatgac ttacatttat cgtggcaaag acgttttgaa aggtcatgtt ggtcaggaag
     5461 aggaagatgg ctccgttgat attcatcaca cccacttgcg tgagttgttg gcccaaaaag
     5521 atgaggccaa tcaagatggc aaccatctgc aaattaaaat gttactcgca tctcattaat
     5581 attcgcgagt taaatgaaat ttatttatct tctgcaaaac tataaactat acatctcatt
     5641 gaaaaaaact aagaagggtg tggaatcagg caattctatc taaaatctag cgaatttgtt
     5701 tccaagaatt gtaagcgtta tatcatttgt ttccactgga accactcacc gttgtctgaa
     5761 taagtcgcac ttttacgagg agtggttcct tgagcaccga cagccaggat cgccacagga
     5821 ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta cccattctcc ggctgctcca
     5881 gtggcttctc cagatttttg gtggccaaca actgctccat atcccgggct actttgctaa
     5941 tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc cgtccgggca
     6001 caacggccaa cacctgtacg taaaagtccg ccggattgta gttggtagga cactgggcac
     6061 ccacgctgga taggagttga gatgttatgt aatactagat acccttaata aacacatcga
     6121 actcactagg aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc taccctgccc
     6181 tcggccatca gaaggatctt gtcaaagagc tcaaacagct cggaagacgg ctgatgaatg
     6241 gtcaggatga cggtcttgcc cttctgcgac agcttcttca gcacctggac gacgctgtgg
     6301 gcggtaaagg agtccagtcc ggaggtgggc tcatcgcaga tcagaagcgg cggatcggtt
     6361 agagcctcgg aggcgaatgc cagacgcttc ctttctccgc cggacagacc tttcaccctg
     6421 ccgggcacac cgatgatcgt gtgctgacat ttgctgagcg aaagctcctg gatcacctga
     6481 tccacgcggg ccactcgctg ccgataggtc agatgtcgtg gcatccgcac catggcttgg
     6541 aaaatcaggt gttccctggc cgttagggag ccgataaaga ggtcatcctg ctggacatag
     6601 gcgcacctgg cctgcatctc cttggcgtcc acaggttggc cattgagcag tcgcatcccg
     6661 gatggcgata cttggatgcc ctgcggcgat cgaaaggcaa gggcattcag cagggtcgtc
     6721 tttccggcac cggaactgcc catcacggcc aaaagttcgc ccggataggc cacgccgcaa
     6781 actgagtttc aaattggtaa ttggaccctt tattaagatt tcacacagat cagccgactg
     6841 cgaatagaaa ctcaccgttc ttgagcaaat gtttcctggg cgccggtatg tgtcgctcgt
     6901 tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc ggctgattga
     6961 ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct agttggccgc
     7021 tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag tttttggcct
     7081 gcccgaagcc ctggttaatg cagctctgcg aagccgctcc gctgtcaccc tgcaatgata
     7141 ggggatctca aatatcaact actagcgtta tgctcatcta accccgaaca aaaagtaccc
     7201 cgaagtatcc tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc
     7261 ttaaaatatc tggttgttat attttttgta aaaaagaata tagtcgaaaa tgaatgcctt
     7321 tagatgtctt gatcatgata tgatctcaaa aattgtctta tatagcgaga acagctacca
     7381 gaataatctg tttcgtgtca ctatttgttt gtgcaattgc ggtttgggat ttttgtgggt
     7441 cgcagttctc acgccgcata caatttgatg ttgcaatcgc agttcctata gatcaagtga
     7501 acttaagatg tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt
     7561 gctgcctccg cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac
     7621 tttcccctgc ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa
     7681 aataagaagc gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc
     7741 ttggagcttc gtcacgaaac cgctgacaaa atgcaactga aggcggacat tgacgctacg
     7801 taacgctaca aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat
     7861 ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg
     7921 agaggtaatc gaaagaacct ggtacatcaa atacccttgg atcgaagtaa atttaaaact
     7981 gatcagataa gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac
     8041 tttccgcaaa aatgggtttt attaacttac atacatacta ggcgcgccca tatgttcggc
     8101 ttgtcgacat gcccgccgtg accgtcgaga acccgctgac gctgccccgc gtatccgcac
     8161 ccgccgacgc cgtcgcacgt cccgtgctca ccgtgaccac cgcgcccagc ggtttcgagg
     8221 gcgagggctt cccggtgcgc cgcgcgttcg ccgggatcaa ctaccgccac ctcgacccgt
     8281 tcatcatgat ggaccagatg ggtgaggtgg agtacgcgcc cggggagccc aagggcacgc
     8341 cctggcaccc gcaccgcggc ttcgagaccg tgacctacat cgtcgacggt aacatgtgag
     8401 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8461 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8521 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8581 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8641 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8701 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8761 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8821 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8881 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8941 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9001 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9061 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9121 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9181 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9241 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9301 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9361 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9421 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9481 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9541 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9601 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9661 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9721 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9781 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9841 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9901 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9961 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10021 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10081 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10141 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10201 ctgacgtc
//
