LOCUS       sequence-123363-        1770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The heterotrimeric G-protein subunit, G-alpha-i isoform 1, tagged 
            with mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: Galphai1-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..1770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(373..375,376..378,379..891)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(418..439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(679..698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1026..1047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgggctgca cactgagcgc tgaggacaag gcggccgtgg agcgcagcaa gatgatcgac
       61 cgcaacctcc gggaggacgg agagaaggca gcgcgcgagg tcaagctgct gctgctgggt
      121 gctggtgaat ccgggaagag cacaattgtg aagcagatga aaattatcca cgaggctggc
      181 tactcagagg aagagtgtaa gcagtacaaa gcagtggtct acagcaacac catccagtcc
      241 atcattgcca tcattagagc tatggggaga ttgaaaatcg actttggaga cgctgctcgt
      301 gcggatgatg ctcgccaact cttcgtgctt gctggggctg cagaggaagg ctttatgacc
      361 gcgaccggtt ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaactac tttagcgaca acgtctatat caccgccgac
      841 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
      901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      961 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1021 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcaccggtga actcgccggc
     1081 gtcataaaga gactgtggaa ggacagcggt gtgcaagcct gcttcaacag atcccgggag
     1141 taccagctga acgattcggc ggcgtactac ctgaatgact tggacagaat agcacaacca
     1201 aattacatcc caacccagca ggatgttctc agaactagag tgaaaacgac gggaattgtg
     1261 gaaacccact ttactttcaa agatcttcat tttaaaatgt ttgacgtggg aggccagaga
     1321 tcagagcgga agaagtggat tcactgcttt gaaggcgtga ctgccatcat cttctgtgtg
     1381 gccctgagtg actatgacct ggttcttgct gaggatgaag aaatgaaccg gatgcacgaa
     1441 agcatgaagc tgttcgatag catatgtaac aacaagtggt ttacggacac atccatcatc
     1501 cttttcctga acaagaagga cctcttcgaa gagaagatca aaaagagtcc cctcacgata
     1561 tgctatccag aatatgcagg ctcaaacaca tatgaagagg cggctgcgta tatccagtgt
     1621 cagtttgaag acctcaataa aaggaaggac acaaaggaaa tttacaccca cttcacttgc
     1681 gccacggata cgaagaatgt gcagtttgtg ttcgatgctg taacggacgt catcataaag
     1741 aataacctaa aagactgtgg tctcttctaa
//
