LOCUS       sequence-123364-        6687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of non-canonical human GLIS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Maekawa M, Yamaguchi K, Nakamura T, Shibukawa R, Kodanaka I, 
            Ichisaka T, Kawamura Y, Mochizuki H, Goshima N, Yamanaka S
  TITLE     Direct reprogramming of somatic cells is promoted by maternal 
            transcription factor Glis1.
  JOURNAL   Nature. 2011  . 474(7350):225-9.
  PUBMED    21654807
REFERENCE   2  (bases 1 to 6687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011  . 474(7350):225-9."
COMMENT     Sequence Label: pMXs-GLIS1
FEATURES             Location/Qualifiers
     source          1..6687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             2045..2514
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    2831..2855
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4737..4761)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4812..5186
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(4858..4874)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(5196..5612)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5212..5228)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5244..5266)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5671..6028
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             6091..6684
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttgcggccg gccgcaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcaa
     1981 ctatggccat ttaatgtaaa tacttaagaa aaaaaaccaa attaattttg atacatgctg
     2041 catgtgaaag acccccgctg acgggtagtc aatcactcag aggagaccct cccaaggaac
     2101 agcgagacca caagtcggat gcaactgcaa gagggtttat tggatacacg ggtacccggg
     2161 cgactcagtc aatcggagga ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag
     2221 ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt agttcaaata
     2281 aggcacaggg tcatttcagg tccttggggc accctggaaa catctgatgg ttctctagaa
     2341 actgctgagg gctggaccgc atctggggac catctgttct tggccctgag ccggggcagg
     2401 aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct gttcttggcc
     2461 ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc
     2521 catctgttcc tgaccttgat ctgaacttct ctattctcag ttatgtattt ttccatgcct
     2581 tgcaaaatgg cgttacttaa gctagcttgc caaacctaca ggtggggtct ttcattcccc
     2641 cctttttctg gagactaaat aaaatctttt attttatcgt cgaccactgt gctggcggcc
     2701 gctcgagttt aaatagtaca taatggattt ccttacgcga aatacgggca gacatggcct
     2761 gcccggttat tattattttt gacaccagac caactggtaa tggtagcgac cggcgctcag
     2821 ctggaatatc accactttgt acaagaaagc tgggtcttag gtgtctgtgt agatggaggg
     2881 gatgtggccc aggcagtggt caaaggctcc attggggaag aagccacagt cctcggggcc
     2941 gctggacacc acatcttcag atggctgctg tggcagcgct gtggggcatg aggcctcagc
     3001 cagggcctca tagcctgtgg caggcaaggg cgtgctgagg ctgtggtagc cattgggccg
     3061 cagggggttg aaaccgtggg tctccccgac cagtccgtcc ccaccggctg ctggttcagc
     3121 catccggtag cagtcgccat aggggaagca actctggatg gagtggaaac tgccctggta
     3181 accttgtggg ctgggcagag gcgggggtgg agggctctgg aagggtgggt aggacggctt
     3241 gctggggagt gtggggaagg gctggccccc cggagaatgg ctctgagagg atgggggcag
     3301 cggcggtggc cccagcccct tcagggggct gactattggt gagaggaggc cgggccccaa
     3361 cccatcccgg gtgctctcag ccatgggcag aggggacaga tggtggtggg aactggtggt
     3421 ggcatccagc gggtggtgcc tggaaggtac gtcgtgcgct gggggcagga ggcccgatgc
     3481 aagtccgtta tggggggtga tggagccagg atacacacct gggagcagct cctggcccag
     3541 gccacagcca cccttgccgt cgctggcagc cagctgtgtg gacgtgtgga gctgctgcag
     3601 gaccagacac tcggtcagga cgtcggcctc ggtgtcaggg cccgcatgca gcttcttacg
     3661 cacctgctgc tctttggctg aatgggcctt gacgtgcttg cggagggagc tggggtctgt
     3721 gtagcgcttg gagcagccag ggatctgaca ggcgtacggc ttcgtgtcta ggtgggtgcg
     3781 ctggtgcttg gcgcggtcgc tggagttgct gaaggccttc tggcaacccg ggtgctggca
     3841 caggtacggc ttctcgcccg tgtggctcct caggtggatc ttgaggttct ccagccgtga
     3901 gaaggccttg ctgcagcctt caaacatgca cttgttgggc ttctcgcccg agtgcactcg
     3961 catgtggatg agcagcttgt agcgggcgtt gaagggcttg tagcggcgca cgcagccagc
     4021 ccagaagcag gtgaagtcct cgcccttgcg ctggtcggtg tggctcttct cgatgtgccg
     4081 caccagctcc tcctgctgct cataggctgc acagcagtcc acccagcggc acgcctgccg
     4141 cccggccacc accctgcctc ccaggcccag ccccaggcct ccaaggcttg ggccaggcgg
     4201 caactgagac aggggatagg ggggcggcag gccctgctgg tgaggcccaa agagctctga
     4261 aaactcatcc gcgggttcct gcttcaggaa gctcgccttc cggcaggctt caagttgcaa
     4321 gctgccctca tggctgtccg tcgatgcagg gccaggccgg gcccgcttgg aagggccccc
     4381 cagatctccc gccagagggg ggctccggag tccatttaca caggtgacca gagacgtctg
     4441 ggaggagcgg atgatggagg tgacgtcgga ggaggcacag ggtgaggagg aggctgggga
     4501 ggtggggggt aggcccaaga cacagcaccg cttcaggctg ccctcgggga ggtgggtctc
     4561 gggctggagg cccaggccag agctgggttc gctgcccaga aggtagcagg aaggcgcagg
     4621 ggtggcgagg cttcggcccg ggaggtccag gtctgggtgc aggccagtgg ccagcgggcc
     4681 ccgacagtgg gcagacaggg atgtgcgggc ctctgccatg gttctatctc cttcgaagcc
     4741 tgcttttttg tacaaacttg tgatttcccg taccaccaca ctgggatcct taattaacta
     4801 gctagatccg gcagtctaga ggatggtcca cccccggggt cggcagcctt cacgtgggcg
     4861 gcgtgtatcc aagctgcgat gccgtctact ttgagggcgg tgggggtggt cagcaggact
     4921 gtgtaaggtc ctttccagcg aggttctagg ttcttagtct ggtgtcggcg gacccacact
     4981 gtgtcgccga ctcggtaagg gtgaggtacc accggtcggt ccagttgttc ttggtaggct
     5041 gccgccagag gtctccagac ttcgtgctgg actaagtaga gagcctgtaa gtgagcttgg
     5101 agagaggggc tgttagtaac tcttgtcatg tcagggtcag ggaagtttac aaggggcggg
     5161 ggtgccccat ataagatctc atatggccat atgggggcgc ctagagaagg agtgagggct
     5221 ggataaaggg aggatcgagg cggggtcgaa cgaggaggtt caagggggag agacggggcg
     5281 gatggaggaa gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca gggagggggg
     5341 tcaaaagcca aggcttccca ggtcacgatg taggggacct ggtctgggtg tccatgcggg
     5401 ccaggtgaaa agaccttgat cttaacctgg gtgatgaggt ctcggttaaa ggtgccgtct
     5461 cgcggccatc cgacgttaaa ggttggccat tctgcagagc agaaggtaac ccaacgtctc
     5521 ttcttgacat ctaccgactg gttgtgagcg atccgctcga catctttcca gtgacctaag
     5581 gtcaaactta agggagtggt aacagtctgg ccctaatttt cagacaaata cagaaacaca
     5641 gtcagacaga gacaacacag aacgatgctg cagcagacaa gacgcgcggc gcggcttcgg
     5701 tcccaaaccg aaagcaaaaa ttcagacgga ggcgggaact gttttaggtt ctcgtctcct
     5761 accagaacca catatccctc ctattagggg ggtgcaccaa agagtccaaa acgatcggga
     5821 tttttggact caggtcgggc cacaaaaacg gcccccgaag tccctgggac gtctcccagg
     5881 gttgcggccg ggtgttccga actcgtcagt tccaccacgg gtccgccaga tacagagcta
     5941 gttagctaac tagtaccgac gcaggcgcat aaaatcagtc atagacacta gacaatcgga
     6001 cagacacaga taagttgctg gccagcttac ctcccggtgg tgggtcggtg gtccctgggc
     6061 aggggtctcc cgatcccgga cgagccccca aatgaaagac ccccgctgac gggtagtcaa
     6121 tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc aactgcaaga
     6181 gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact ggcgccccga
     6241 gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga acagaagcga
     6301 gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc cttggggcac
     6361 cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat ctggggacca
     6421 tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca
     6481 tattcagctg ttccatctgt tcttggccct gagccggggc aggaactgct taccacagat
     6541 atcctgtttg gcccatattc agctgttcca tctgttcctg accttgatct gaacttttct
     6601 attctcagtt atgtattttt ccatgccttg caaaatggcg ttacttaagc tagcttgcca
     6661 aacctacagg tggggtcttt cattatg
//
