LOCUS       sequence-12338-e        9790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPp65ADZpUw
FEATURES             Location/Qualifiers
     source          1..9790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1999..2019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2470..2841
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     primer_bind     complement(3285..3301)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3285..3301)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4312..4926,4997..5128,5332..5647,5709..6363,
                     6438..6711,7113..7184))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7636..7654
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    7879..7948
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8035..8623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8115..8134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8794..9585,9586..9654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9417..9436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9655..9759)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accaaaacat ggataaagcg
     1981 gaattaattc ccgagcctcc aaaaaagaag agaaaggtcg aattgggatc cacgccgatg
     2041 gagttccagt acctgcccga tacggatgac cgtcaccgta tcgaagaaaa gcggaagcga
     2101 acctatgaaa ccttcaagtc catcatgaaa aagtccccct tctcgggccc cacggacccg
     2161 cgccccccgc cccgtcgtat tgcggttcct tcgcgcagca gtgccagcgt ccccaaaccc
     2221 gcaccgcagc cctacccgtt cacttcctcc cttagcacga ttaactatga tgagttcccc
     2281 acgatggtgt tccccagtgg acaaatttcc caggcatcgg cactggctcc ggccccaccg
     2341 caagtcctcc cccaggcgcc cgctccggca ccggctcccg caatggtgag tgctctggcc
     2401 caggcccccg ctccagtccc cgtgctggcg cctggacccc cacaggcagt tgcccctcct
     2461 gctccgaaac caacgcaggc gggcgaagga accctgagcg aggccctctt gcagcttcag
     2521 ttcgatgacg aagacttggg agccctgctg ggtaacagca cagaccctgc cgtattcacc
     2581 gatctcgcat ccgtggacaa cagcgagttt cagcagctct tgaatcaggg aatcccggtc
     2641 gcacctcata ccacagagcc catgctgatg gaatacccgg aggctatcac gcgactggtg
     2701 accggcgcac agcgaccacc cgatccagcc cctgccccac tgggtgcccc gggtttgccc
     2761 aatggcctcc tcagcggcga tgaggatttc tccagcatcg ctgatatgga tttctccgct
     2821 ttgctgagcc agataagctc cgctagcgga ggaggtggtg gaggtggagg tggaggtact
     2881 agtctggaga tcgaggccgc cttcctggag cgcgagaaca ccgccctgga gacccgcgtg
     2941 gccgagctgc gccagcgcgt gcagcgcctg cgcaaccgcg tgagccagta ccgcacccgc
     3001 tacggccccc tgggcggcgg caagtaaaag cttatcgata ccgtcgacta aagccaaata
     3061 gaaaattatt cagttcctgg cttaagtttt taaaagtgat attatttatt tggttgtaac
     3121 caaccaaaag aatgtaaata actaatacat aattatgtta gttttaagtt agcaacaaat
     3181 tgattttagc tatattagct acttggttaa taaatagaat atatttattt aaagataatt
     3241 gcgtttttat tgtcagggag tgagtttgct taaaaactcg tttagatcca ctagttctag
     3301 agcggccgct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
     3361 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
     3421 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     3481 gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggaggcgc
     3541 gccctagttc cagtgaaatc caagcatttt ctaaattaaa tgtattctta ttattatagt
     3601 tgttattttt gatatatata aacaacacta ttatgcccac catttttttg agatgcatct
     3661 acacaaggaa caaacactgg atgtcacttt cagttcaaat tgtaacgcta atcactccga
     3721 acaggtcaca aaaaattacc ttaaaaagtc ataatattaa attagaataa atatagctgt
     3781 gagggaaata tatacaaata tattggagca aataaattgt acatacaaat atttattact
     3841 aatttctatt gagacgaaat gaaccactcg gaaccatttg agcgaaccga atcgcgcgga
     3901 actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg gagagcgggt
     3961 gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt atgaagaggg
     4021 cgcgcgattt gaaaagtatg tatataaaaa atatatcccg gtgttttatg tagcgataaa
     4081 cgagtttttg atgtaaggta tgcaggtgtg taagtctttt ggttagaaga caaatccaaa
     4141 gtctacttgt ggggatgttc gaaggggaaa tacttgtatt ctataggtca tatcttgttt
     4201 ttattggcac aaatataatt acattagctt tttgaggggg caataaacag taaacacgat
     4261 ggtaataatg gtaaaaaaaa aaaacaagca gttatttcgg atatatgtcg gctactcctt
     4321 gcgtcgggcc cgaagtctta gagccagata tgcgagcacc cggaagctca cgatgagaat
     4381 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
     4441 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
     4501 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
     4561 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagc cgccaaagag
     4621 caggaatggt atgataaccg gcggacccac agacagcgcc atcgaggtcg aggagctggc
     4681 gcaggatatt agatatccga aggacgttga cacattggcc accagagtga ccagcgccag
     4741 gcagttgaag aagtgcagca ctccggcccg cagtccgatc atcggatagg caatcgccgt
     4801 gaagaccagt ggcactgtga gaaaaagcgg caattcggca atcgttttgc ccagaaagta
     4861 tgtgtcacag cgataaagtc gacttcgggc ctccctcata aaaactggca gctctgaggt
     4921 gaacacctaa atcgaatcga ttcattagaa agttagtaaa ttattgaaat gcaaatgtat
     4981 tctaaacatg acttacattt atcgtggcaa agacgttttg aaaggtcatg ttggtcagga
     5041 agaggaagat ggctccgttg atattcatca cacccacttg cgtgagttgt tggcccaaaa
     5101 agatgaggcc aatcaagatg gcaaccatct gcaaattaaa atgttactcg catctcatta
     5161 atattcgcga gttaaatgaa atttatttat cttctgcaaa actataaact atacatctca
     5221 ttgaaaaaaa ctaagaaggg tgtggaatca ggcaattcta tctaaaatct agcgaatttg
     5281 tttccaagaa ttgtaagcgt tatatcattt gtttccactg gaaccactca ccgttgtctg
     5341 aataagtcgc acttttacga ggagtggttc cttgagcacc gacagccagg atcgccacag
     5401 gaccgcccgg aactgcatga accaggtggc cttgtaggtg tacccattct ccggctgctc
     5461 cagtggcttc tccagatttt tggtggccaa caactgctcc atatcccggg ctactttgct
     5521 aatggcaaaa ttgtcgcata tcttggcgat ccgatcacgg gactcgatct cccgtccggg
     5581 cacaacggcc aacacctgta cgtaaaagtc cgccggattg tagttggtag gacactgggc
     5641 acccacgctg gataggagtt gagatgttat gtaatactag atacccttaa taaacacatc
     5701 gaactcacta ggaaaagaag tcgacggctt cgctgggagt gcccaagaaa gctaccctgc
     5761 cctcggccat cagaaggatc ttgtcaaaga gctcaaacag ctcggaagac ggctgatgaa
     5821 tggtcaggat gacggtcttg cccttctgcg acagcttctt cagcacctgg acgacgctgt
     5881 gggcggtaaa ggagtccagt ccggaggtgg gctcatcgca gatcagaagc ggcggatcgg
     5941 ttagagcctc ggaggcgaat gccagacgct tcctttctcc gccggacaga cctttcaccc
     6001 tgccgggcac accgatgatc gtgtgctgac atttgctgag cgaaagctcc tggatcacct
     6061 gatccacgcg ggccactcgc tgccgatagg tcagatgtcg tggcatccgc accatggctt
     6121 ggaaaatcag gtgttccctg gccgttaggg agccgataaa gaggtcatcc tgctggacat
     6181 aggcgcacct ggcctgcatc tccttggcgt ccacaggttg gccattgagc agtcgcatcc
     6241 cggatggcga tacttggatg ccctgcggcg atcgaaaggc aagggcattc agcagggtcg
     6301 tctttccggc accggaactg cccatcacgg ccaaaagttc gcccggatag gccacgccgc
     6361 aaactgagtt tcaaattggt aattggaccc tttattaaga tttcacacag atcagccgac
     6421 tgcgaataga aactcaccgt tcttgagcaa atgtttcctg ggcgccggta tgtgtcgctc
     6481 gttgcagaat agtccgcgtg tccggttgac cagctgccgc catccggagc ccggctgatt
     6541 gaccgcccca aagatgtcca tattgtgcca ggcataggtg aggttctcgg ctagttggcc
     6601 gctccctgaa ccggagtcct ccggcggact gggtggccgg agcgtgccgt agtttttggc
     6661 ctgcccgaag ccctggttaa tgcagctctg cgaagccgct ccgctgtcac cctgcaatga
     6721 taggggatct caaatatcaa ctactagcgt tatgctcatc taaccccgaa caaaaagtac
     6781 cccgaagtat cctacgaagt aggtttatac ttttatttat tttttgtgca tctaggatca
     6841 gcttaaaata tctggttgtt atattttttg taaaaaagaa tatagtcgaa aatgaatgcc
     6901 tttagatgtc ttgatcatga tatgatctca aaaattgtct tatatagcga gaacagctac
     6961 cagaataatc tgtttcgtgt cactatttgt ttgtgcaatt gcggtttggg atttttgtgg
     7021 gtcgcagttc tcacgccgca tacaatttga tgttgcaatc gcagttccta tagatcaagt
     7081 gaacttaaga tgtatgcaca tgtactactc acattgttca gatgctcggc agatgggtgt
     7141 ttgctgcctc cgcgaattaa tagctcctga tcctcttggc ccattgccgg gatttttcac
     7201 actttcccct gcttacccac ccaaaaccaa tcaccacccc aatcactcaa aaaacaaaca
     7261 aaaataagaa gcgagaggag ttttggcaca gcactttgtg tttaattgat ggcgtaaacc
     7321 gcttggagct tcgtcacgaa accgctgaca aaatgcaact gaaggcggac attgacgcta
     7381 cgtaacgcta caaacggtgg cgaaagagat agcggacgca gcggcgaaag agacggcgat
     7441 atttctgtgg acagagaagg aggcaaacag cgctgacttt gagtggaatg tcattttgag
     7501 tgagaggtaa tcgaaagaac ctggtacatc aaataccctt ggatcgaagt aaatttaaaa
     7561 ctgatcagat aagttcaatg atatccagtg cagtaaaaaa aaaaaatgtt ttttttatct
     7621 actttccgca aaaatgggtt ttattaactt acatacatac taggcgcgcc catatgttcg
     7681 gcttgtcgac atgcccgccg tgaccgtcga gaacccgctg acgctgcccc gcgtatccgc
     7741 acccgccgac gccgtcgcac gtcccgtgct caccgtgacc accgcgccca gcggtttcga
     7801 gggcgagggc ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc acctcgaccc
     7861 gttcatcatg atggaccaga tgggtgaggt ggagtacgcg cccggggagc ccaagggcac
     7921 gccctggcac ccgcaccgcg gcttcgagac cgtgacctac atcgtcgacg gtaacatgtg
     7981 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8041 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8101 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8161 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8221 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8281 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8341 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8401 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8461 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8521 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8581 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8641 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8701 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8761 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9781 acctgacgtc
//
