LOCUS       sequence-123451-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing ZeoR for generation of GMAP-compatible 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO ZeoR
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(380..682)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(482..501)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     805..827
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     819..836
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     820..836
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     843..862
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        843..861
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
       61 cagagcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
      121 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
      181 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
      241 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
      301 agcaccgttt ctgcggactg gctttctacg tgaaaaggat ctaggtgaag atcctttttg
      361 ataatctcat gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
      421 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg
      481 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
      541 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
      601 ccggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
      661 tataggtgta aaccttaaac tgccgtacgt ataggctgcg caactgttgg gaagggcgat
      721 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
      781 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
      841 tgtaatacga ctcactatag ggcgaattgg gccctctaga tgcatgctcg agcggccgcc
      901 agtgtgatgg a
//
