LOCUS       sequence-123453-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO backbone for GMAP promoters with sites #1 and #4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO 1-4
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(375..677)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(477..496)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     800..822
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     814..831
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     815..831
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     838..857
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        838..856
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctgcggcatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagag
       61 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
      121 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
      181 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
      241 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
      301 cgtttctgcg gactggcttt ctacgtgaaa aggatctagg tgaagatcct ttttgataat
      361 ctcatgcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
      421 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata
      481 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
      541 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgccccggc
      601 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
      661 gtgtaaacct taaactgccg tacgtatagg ctgcgcaact gttgggaagg gcgatcggtg
      721 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      781 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
      841 tacgactcac tatagggcga attgggccct ctagatgcag gctcgagcgg ccgccagtgt
      901 gatggatatc tg
//
