LOCUS       sequence-123454-        6059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Samuelson AV, Lowe SW
  TITLE     Selective induction of p53 and chemosensitivity in RB-deficient 
            cells by E1A mutants unable to bind the RB-related proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Oct 28. 94(22):12094-9.
  PUBMED    9342368
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Oct 28. 94(22):12094-9."
COMMENT     Sequence Label: pBabe 12S E1A
FEATURES             Location/Qualifiers
     source          1..6059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(810..1409)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(893..913)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1390..1409
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1419..1748)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1433..1568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1733..1753
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(2784..3200)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2800..2816)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2832..2854)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3401..3600
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3665..4134
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4165..4269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4270..4338,4339..5130)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4488..4507)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5290..5878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5779..5798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6032..6049
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgatgcat ggggtcgtgc gctcctttcg
      781 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
      841 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
      901 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
      961 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     1021 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     1081 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     1141 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     1201 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     1261 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     1321 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     1381 aggcgcaccg tgggcttgta ctcggtcatg gtaagctttt tgcaaaagcc taggcctcca
     1441 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     1501 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1561 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1621 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     1681 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     1741 tgacacacat tccacagggt cgaccactgt gctggcgaat tcctacgtac caccacactg
     1801 ggatcccgaa ccacacacgc aatcacaggt ttacacctta tggcctgggg cgtttacagc
     1861 tcaagtccaa aggttgccca ggctcgttaa gcaagtcctc gatacattcc acagcctggc
     1921 gacgcccacc aactctcacg gcaactggtt taatggggca cagcgggacc accgggtgta
     1981 tctcaggagg tgtgttagaa ggaccggagt cacagctatc cgtactacta ttgcattctc
     2041 tagacacagg tgatgtcggg cgtctcagga tagcaggcgc cattttagga cggcgggtag
     2101 gtcttgcagg ctccggttct ggctcgggct caggctcagg ttcagacaca ggaccctctt
     2161 catcctcgtc gtcactgggt ggaaagccag cctcgtggca ggtaagatcg atcacctccg
     2221 gtacaaggtt tggcatagaa accggaccca aggctctctg ctccggctgc tcgggctgcc
     2281 gggaaaggtg aggcggctcc ggagaaccgg gcgccggcgg aaaagtgagt aagtcaatcc
     2341 cttcctgcac cgccaacatt acagagtcgg gaaaaatctg cgaaaccgcc tcctcgttgg
     2401 gatcttcggg ggccgtcacg tctaaatcat acagttcgtg aagggtaggt ggttcaaaat
     2461 ggctaggagg tggaagatta tcagccagta cctcttcgat cagctggtcc aaaagactgg
     2521 cggccatttc ttcggtaata acacctccgt ggcagataat atgtctcatt ttcagtcccg
     2581 gtgtcggagc ggctcggagg agaaaactct actcgctggc actcaagagt ggcctcttga
     2641 ggaactcacc gggtataaat acactacacg tcagctgact ataataataa aacgccaact
     2701 ttgacccgga acgcggaaaa cacctgagaa aaacacctgg gcgagtctcc acgtaaacgg
     2761 tcaaagtccc ccgggatccg gccggcgcct agagaaggag tgagggctgg ataaagggag
     2821 gatcgaggcg gggtcgaacg aggaggttca agggggagag acggggcgga tggaggaaga
     2881 ggaggcggag gcttagggtg tacaaagggc ttgacccagg gaggggggtc aaaagccaag
     2941 gcttcccagg tcacgatgta ggggacctgg tctgggtgtc catgcgggcc aggtgaaaag
     3001 accttgatct taacctgggt gatgaggtct cggttaaagg tgccgtctcg cggccatccg
     3061 acgttaaagg ttggccattc tgcagagcag aaggtaaccc aacgtctctt cttgacatct
     3121 accgactggt tgtgagcgat ccgctcgaca tctttccagt gacctaaggt caaacttaag
     3181 ggagtggtaa cagtctggcc ctaattttca gacaaataca gaaacacagt cagacagaga
     3241 caacacagaa cgatgctgca gcgctgcagc agacaagacg cgcggcttcg gttccaaacc
     3301 gaaagcaaaa attcagacgg aggcgggaac tgttttaggt tctcgtctcc taccagaacc
     3361 acatatcctg acggggtcgg attccacatc gactcccttc ctcaggtcgg gccacaaaaa
     3421 cggcccccaa agtccctggg acgtctccca gggttgcggc cgggtgttca gaactcgtca
     3481 gttccaccac gggtccgcca gatacagagc tagttagcta actagtaccg acgcaggcgc
     3541 ataaaatcag tcatagacac tagacaatcg gacagacaca gataagttgc tggccagctt
     3601 gcctcccggt ggtgggtcgg tggtccctgg gcaggggtct cccgatcccg gacgagcccc
     3661 caaatgaaag acccccgctg acgggtagtc aatcactcag aggagaccct cccaaggaac
     3721 agcgagacca caagtcggat gcaactgcaa gagggtttat tggatacacg ggtacccggg
     3781 cgactcagtc aatcggagga ctggcgcccc gagtgagggg ttgtgggctc ttttattgag
     3841 ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt agttcaaata
     3901 aggcacaggg tcatttcagg tccttggggc accctggaaa catctgatgg ttctctagaa
     3961 actgctgagg gctggaccgc atctggggac catctgttct tggccctgag ccggggcagg
     4021 aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct gttcttggcc
     4081 ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat tcaggctgca
     4141 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4201 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4261 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4321 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4381 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4441 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4501 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4561 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4621 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4681 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4741 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4801 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4861 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4921 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4981 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5041 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5101 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5161 tagattgatt tgcggccgca aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5221 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5281 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5341 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5401 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5461 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5521 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5581 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5641 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5701 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5761 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5821 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5881 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5941 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6001 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
