LOCUS       sequence-123460-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 
            and H4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Shim Y, Duan MR, Chen X, Smerdon MJ, Min JH
  TITLE     Polycistronic coexpression and nondenaturing purification of histone
            octamers.
  JOURNAL   Anal Biochem. 2012 Aug 15;427(2):190-2. doi: 
            10.1016/j.ab.2012.05.006. Epub 2012 May 19.
  PUBMED    22617796
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2012.05.006"; journalName: "Anal Biochem"; date: 
            "2012-08-15- 15"; volume: "427"; issue: "2"; pages: "190-2"
COMMENT     Sequence Label: pET29a-YS14
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(60..77)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             403..425
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             858..880
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagg
       61 ctgccgcgcg gcaccaggcc gctgctacca ccgaaaccgt acagggtacg accctgacgt
      121 ttcagagcgt aaacaacgtc catagcggta acggttttac gtttagcgtg ttcggtgtag
      181 gtaacagcgt cacggataac gttttccagg aaaactttca gaacaccgcg ggtttcttcg
      241 tagatcagac cggagatacg tttaacacca ccacgacgag ccagacgacg gatagccggc
      301 ttggtgatac cctggatgtt gtcacgcaga actttacggt gacgtttagc accaccttta
      361 cccagacctt taccaccttt accacgacca gacatatgta tatctccttc ttaaagttaa
      421 acaaaattat tgcggccgcc taagccctct cgcctcggat tctgcgggcc agctggatgt
      481 ccttgggcat gatggtgacc ctcttggcgt ggatggcaca caggttggtg tcctcaaaga
      541 gagcgaccag ataagcctcg ctggcctcct gcagagccat aacggccgag ctctggaagc
      601 gcaggtcggt cttgaagtcc tgagcgatct cccggaccag gcgctggaaa ggcagtttgc
      661 ggatgagcag ctcggtggat ttctggtagc ggcggatctc gcggagagcg actgtgccgg
      721 gccggtaacg gtgaggtttc ttgactccgc cggtagcagg agcggacttc ctggctgcct
      781 tggtggccag ctgcttgcgg ggagccttcc ctccggtgga tttacgggcg gtctgcttgg
      841 tacgggccat atgtatatct ccttcttaaa gttaaacaaa attattgtcg acttacttgg
      901 cgctggtgta cttggtgaca gccttggtgc cctcggacac ggcgtgtttg gccaactccc
      961 caggcagcag cagtcggacc gcggtctgga tctcccggga ggtgatggtg gagcgcttgt
     1021 tgtaatgagc caggcgggag gcttcccctg cgatgcgctc aaacacatcg ttgacaaagg
     1081 agttcatgat gctcatggcc ttggac
//
