LOCUS       sequence-123461-        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 
            and H4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Shim Y, Duan MR, Chen X, Smerdon MJ, Min JH
  TITLE     Polycistronic coexpression and nondenaturing purification of histone
            octamers.
  JOURNAL   Anal Biochem. 2012 Aug 15;427(2):190-2. doi: 
            10.1016/j.ab.2012.05.006. Epub 2012 May 19.
  PUBMED    22617796
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2012.05.006"; journalName: "Anal Biochem"; date: 
            "2012-08-15- 15"; volume: "427"; issue: "2"; pages: "190-2"
COMMENT     Sequence Label: pET29a-YS14
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..91
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        72..90
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    91..115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             130..152
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             163..207
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             217..234
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             241..258
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             670..692
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tcgatctcga
       61 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      121 agaaataatt ttgtttaact ttaagaagga gatatacata tgaaagaaac cgctgctgct
      181 aaattcgaac gccagcacat ggacagccca gatctgcacc accaccacca ccacggtacc
      241 ctggtgccac gcggttccat gggtatgtca ggaagaggca aacaaggcgg taaaacccgc
      301 gctaaggcca agactcgctc atctcgggct gggctacagt tccctgttgg ccgtgttcac
      361 cggctgttaa ggaaaggcaa ttatgcagag cgggtgggag ctggagctcc agtctatctg
      421 gctgcagtgt tggagtatct gaccgctgag attttggaat tggccgggaa tgcggcccgt
      481 gataacaaga agactcgcat tatccccaga cacctgcagc tcgctgtgcg caacgatgag
      541 gaactgaaca aactgctcgg aagagtcact atcgctcagg gcggggtcct gcccaacatc
      601 cagtccgtgc tgctgcccaa gaaaaccgag agttccaagt cggccaagag caagtgagaa
      661 ttcaataatt ttgtttaact ttaagaagga gatatacata tggccaagtc cgctccagcc
      721 ccgaagaaag gctccaagaa agcggtgacc aagactcaga agaaagacgg gaaaaagcgc
      781 aggaagacaa ggaaggagag ttatgccatt tacgtgtaca aggtgctgaa gcaggtgcac
      841 cccgataccg gcatctcgtc caaggccatg agcatcatga actcctttgt caacgatgtg
      901 tttgagcgca tcgcagggga agcctcccgc ctggctcatt acaacaagcg ctccaccatc
      961 acctcccggg agatccagac cgcggtccga ctgctgctgc ctggggagtt ggccaaacac
     1021 gccgtgtccg agggcaccaa ggctgtcacc aagtacacca gcgccaagta agtcgacaat
     1081 aattttgttt aactttaaga agga
//
