LOCUS       sequence-12347-e        8619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 8619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-GAL4AD
FEATURES             Location/Qualifiers
     source          1..8619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    689..758
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     949..965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1118..1138
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1154..1495
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     promoter        1501..1519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1526..1535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1538..1564
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2039..2072)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2257..2275)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2727..2798,3200..3473,3548..4202,4264..4579,4783..4914,
                     4985..5599)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(6373..6406)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6431..6451)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6431..6449)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6470..6486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6470..6486)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6483..6505)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6494..6510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6518..6524,6525..6542,6543..6548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6563..6584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6701..6718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6872..7460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6952..6971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7631..8422,8423..8491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8254..8273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8492..8596)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
      721 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
      781 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
      841 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
      901 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg aggtcgacgg
      961 tatcgataag cttaccgaag tatacactta aattcagtgc acgtttgctt gttgagagga
     1021 aaggttgtgt gcggacgaat ttttttttga aaacattaac ccttacgtgg atccgcggcc
     1081 gcaaaacatg gataaagcgg aattaattcc cgagcctcca aaaaagaaga gaaaggtcga
     1141 attgggtacc gccgccaatt ttaatcaaag tgggaatatt gctgatagct cattgtcctt
     1201 cactttcact aacagtagca acggtccgaa cctcataaca actcaaacaa attctcaagc
     1261 gctttcacaa ccaattgcct cctctaacgt tcatgataac ttcatgaata atgaaatcac
     1321 ggctagtaaa attgatgatg gtaataattc aaaaccactg tcacctggtt ggacggacca
     1381 aactgcgtat aacgcgtttg gaatcactac agggatgttt aataccacta caatggatga
     1441 tgtatataac tatctattcg atgatgaaga taccccacca aacccaaaaa aagagatctt
     1501 taatacgact cactataggg cgagcgccgc catggagtac ccatacgacg taccagatta
     1561 cgctggagga ggtggtggag gtggaggtgg aggtactagt ctggagatcg aggccgcctt
     1621 cctggagcgc gagaacaccg ccctggagac ccgcgtggcc gagctgcgcc agcgcgtgca
     1681 gcgcctgcgc aaccgcgtga gccagaaccg cacccgctac ggccccctgg gcggcggcaa
     1741 gtaaggcgcg cctctagatc gataccgtcg actaaagcca aatagaaaat tattcagttc
     1801 ctggcttaag tttttaaaag tgatattatt tatttggttg taaccaacca aaagaatgta
     1861 aataactaat acataattat gttagtttta agttagcaac aaattgattt tagctatatt
     1921 agctacttgg ttaataaata gaatatattt atttaaagat aattgcgttt ttattgtcag
     1981 ggagtgagtt tgcttaaaaa ctcgtttaga tccactagct taattaagca tgcactagat
     2041 aacttcgtat agcatacatt atacgaagtt atagcttctg atggaattag aacttggcaa
     2101 aacaatactg agaatgaagt gtatgtggaa cagaggctgc tgatctcgtt cttcaggcta
     2161 tgaaactgac acatttggaa accacagtac ttagaaccac aaagtgggaa tcaagagaaa
     2221 aacaatgatc ccacgagaga tcgaattcta gtatgtatgt aagttaataa aacccatttt
     2281 tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat cattgaactt
     2341 atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag gttctttcga
     2401 ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt
     2461 ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg
     2521 tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt ttcgtgacga
     2581 agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc
     2641 ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc
     2701 aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg
     2761 gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca
     2821 tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga
     2881 gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg acacgaaaca
     2941 gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata tcatgatcaa
     3001 gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat aacaaccaga
     3061 tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg
     3121 atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     3181 agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg
     3241 cttcgggcag gccaaaaact acggcacgct ccggccaccc agtccgccgg aggactccgg
     3301 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
     3361 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
     3421 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
     3481 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
     3541 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
     3601 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
     3661 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
     3721 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
     3781 ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
     3841 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
     3901 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
     3961 gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc
     4021 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
     4081 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
     4141 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
     4201 tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc
     4261 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     4321 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     4381 ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga
     4441 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     4501 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     4561 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     4621 ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag
     4681 tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac
     4741 tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt
     4801 ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc
     4861 atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt
     4921 catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat
     4981 ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg
     5041 ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc
     5101 actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt
     5161 cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct
     5221 aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat
     5281 accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa
     5341 atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg
     5401 ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc
     5461 gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt
     5521 gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     5581 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac
     5641 cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt
     5701 gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc
     5761 acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat
     5821 caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata catacttttc
     5881 aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc
     5941 gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac
     6001 tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcgtctc
     6061 aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata
     6121 tatttccctc acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt
     6181 tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg
     6241 ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc
     6301 aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg gatttcactg
     6361 gaactagaat tcataacttc gtatagcata cattatacga agttatggat ctgagctcca
     6421 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     6481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8581 aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
