LOCUS       sequence-123474-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral backbone for use with GMAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: RV 2-5
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     206..223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    340..361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(376..381,382..399,400..406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    414..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      241 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      301 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      361 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      421 cggataacaa tttcacaatt ctccccagct ctttctgcgc tgtccataat gaactatttc
      481 aggagtattg gagtatgtgg attcaaaaca gtggctaatt gagtcaggat tacttaatat
      541 tcgactccta ccctcagaat gttgttctcc aacttcaaga aactgttacc catgaaagac
      601 ccccgctgac gggtagtcaa tcactcagag gagaccctcc caaggaacag cgagaccaca
      661 agtcggatgc aactgcaaga gggtttattg gatacacggg tacccgggcg acgcagtcta
      721 tcggaggact ggcgcgccga gtgaggggtt gtgggctctt ttattgagct cggggagcag
      781 aagcgcgcga acagaagcga gaagcgaact gattggttag ttcaaataag gcacagggtc
      841 atttcaggtc cttggggcac cctggaaaca tctgatggtt ctctagaaac tgctgagggc
      901 gggaccgcat ctggggacca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca
      961 cagatatcct gtttggccca tattctgctg tctctctgtt cctaaccttg atctgaactt
     1021 ctctattctc agttatgtat tttccatgcc ttgcaaaatg gcgttact
//
