LOCUS       sequence-12350-e        9406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-LexA
FEATURES             Location/Qualifiers
     source          1..9406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    689..758
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     949..965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(1139..1390,1391..1744)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     1682..1703
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             1790..2023
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(2175..2194)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2219..2267
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(2826..2859)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3044..3062)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3514..3585,3987..4260,4335..4989,5051..5366,5570..5701,
                     5772..6386)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(7160..7193)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7218..7238)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7218..7236)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7257..7273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7257..7273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7270..7292)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7281..7297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7305..7311,7312..7329,7330..7335))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7350..7371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7488..7505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7659..8247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7739..7758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8418..9209,9210..9278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9041..9060
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9279..9383)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
      721 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
      781 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
      841 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
      901 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg aggtcgacgg
      961 tatcgataag cttaccgaag tatacactta aattcagtgc acgtttgctt gttgagagga
     1021 aaggttgtgt gcggacgaat ttttttttga aaacattaac ccttacgtgg atccgcggcc
     1081 gcgcatggta ccgggccccc cctcgagctc gcgaaagctt gaattaattc cgggcggaat
     1141 gaaagcgtta acggccaggc aacaagaggt gtttgatctc atccgtgatc acatcagcca
     1201 gacaggtatg ccgccgacgc gtgcggaaat cgcgcagcgt ttggggttcc gttccccaaa
     1261 cgcggctgaa gaacatctga aggcgctggc acgcaaaggc gttattgaaa ttgtttccgg
     1321 cgcatcacgc gggattcgtc tgttgcagga agaggaagaa gggttgccgc tggtaggtcg
     1381 tgtggctgcc ggtgaaccac ttctggcgca acagcatatt gaaggtcatt atcaggtcga
     1441 tccttcctta ttcaagccga atgctgattt cctgctgcgc gtcagcggga tgtcgatgaa
     1501 agatatcggc attatggatg gtgacttgct ggcagtgcat aaaactcagg atgtacgtaa
     1561 cggtcaggtc gttgtcgcac gtattgatga cgaggttacc gttaagcgcc tgaaaaaaca
     1621 gggcaataaa gtcgaactgt tgccagaaaa tagcgagttt aaaccaattg tcgtagatct
     1681 tcgtcagcag agcttcacca ttgaagggct ggcggttggg gttattcgca acggcgactg
     1741 gctggaattc ccggggatcc gtcgacctgc aggaattccc ggggatctgg cccccccgac
     1801 cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc
     1861 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
     1921 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
     1981 gcagatgttt accgatgccc ttggaattga cgagtacggt gggtaggggg cgcgaccgga
     2041 cccgcatccc ccgtctgggt tttcccctcc cgtccccggt tcgtatccac aataaacacg
     2101 agcacataca ttacaaaaac ctgcggttgt cgtctgatta tttggtggat ccgggggaga
     2161 tgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     2221 gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt
     2281 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     2341 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     2401 agccaacgtc ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg
     2461 tcccccatgg ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt
     2521 caggtccacg acccaagctt ctagatcgat accgtcgact aaagccaaat agaaaattat
     2581 tcagttcctg gcttaagttt ttaaaagtga tattatttat ttggttgtaa ccaaccaaaa
     2641 gaatgtaaat aactaataca taattatgtt agttttaagt tagcaacaaa ttgattttag
     2701 ctatattagc tacttggtta ataaatagaa tatatttatt taaagataat tgcgttttta
     2761 ttgtcaggga gtgagtttgc ttaaaaactc gtttagatcc actagcttaa ttaagcatgc
     2821 actagataac ttcgtatagc atacattata cgaagttata gcttctgatg gaattagaac
     2881 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctt
     2941 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca
     3001 agagaaaaac aatgatccca cgagagatcg aattctagta tgtatgtaag ttaataaaac
     3061 ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat
     3121 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt
     3181 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
     3241 tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca
     3301 ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc
     3361 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
     3421 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
     3481 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
     3541 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
     3601 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
     3661 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca
     3721 cgaaacagat tattctggta gctgttctcg ctatataaga caatttttga gatcatatca
     3781 tgatcaagac atctaaaggc attcattttc gactatattc ttttttacaa aaaatataac
     3841 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
     3901 tcgtaggata cttcggggta ctttttgttc ggggttagat gagcataacg cttgtagttg
     3961 atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta
     4021 accagggctt cgggcaggcc aaaaactacg gcacgctccg gccacccagt ccgccggagg
     4081 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     4141 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     4201 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     4261 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     4321 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     4381 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     4441 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     4501 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     4561 ggaacacctg attttccaag ccatggtgcg gatgccacga catctgacct atcggcagcg
     4621 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     4681 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     4741 cgcctccgag gctctaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     4801 ggactccttt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     4861 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     4921 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     4981 cttttcctag tgagttcgat gtgtttatta agggtatcta gtattacata acatctcaac
     5041 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
     5101 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
     5161 gcgacaattt tgccattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa
     5221 atctggagaa gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca
     5281 tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg
     5341 taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg
     5401 cttacaattc ttggaaacaa attcgctaga ttttagatag aattgcctga ttccacaccc
     5461 ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaagata aataaatttc
     5521 atttaactcg cgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat
     5581 cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa
     5641 cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa
     5701 tgtaagtcat gtttagaata catttgcatt tcaataattt actaactttc taatgaatcg
     5761 attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac
     5821 tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattgccg ctttttctca
     5881 cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc
     5941 tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg
     6001 gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg
     6061 ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat
     6121 acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta
     6181 accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc
     6241 ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg
     6301 actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa
     6361 gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt
     6421 tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat
     6481 tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa
     6541 catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac
     6601 cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat
     6661 acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg
     6721 ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg
     6781 gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt
     6841 tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat
     6901 ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt
     6961 aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca
     7021 gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat
     7081 atatatcaaa aataacaact ataataataa gaatacattt aatttagaaa atgcttggat
     7141 ttcactggaa ctagaattca taacttcgta tagcatacat tatacgaagt tatggatctg
     7201 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     7261 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7321 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7381 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7441 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8341 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8401 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8461 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8521 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8581 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8641 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8701 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8761 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8821 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8881 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8941 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9001 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9061 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
