LOCUS       sequence-123519-         396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental plasmid for transcription of RNAs with a GlmS/M-domain 
            3'-tag for native purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Batey RT, Kieft JS
  TITLE     Improved native affinity purification of RNA.
  JOURNAL   RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4.
  PUBMED    17548432
REFERENCE   2  (bases 1 to 396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2007-08"; volume: "13(8)"; pages: "1384-9. Epub 2007 Jun 4"
COMMENT     Sequence Label: pRAV24
FEATURES             Location/Qualifiers
     source          1..396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      343..390
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattctaat acgactcact atagggtcgc tcaaacatta agtggtgttg tgcgaaaaga
       61 atctcgaaga caagccggca tagcgcccga actaccggta ccggtagttg acgaggatgg
      121 aggttatcga attttcggcg gatgcctccc ggctgagtgt gcagatcaca gccgtaagga
      181 tttcttcaaa ccaagggggt gactccttga acaaagagaa atcacatgat cttccaaaaa
      241 tctagactgt gcatcgggtc aggactgaaa ggtagcagcc ctgggcagtt ttttcctgtg
      301 catcgggtca ggaccttcgg gtagcagccc tgggcaggat ccctagcata accccttggg
      361 gcctctaaac gggtcttgag gggttttttg aagctt
//
