LOCUS       sequence-12352-e       12075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXL-BACII-attPGAL4LWL
FEATURES             Location/Qualifiers
     source          1..12075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   2641..2675
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2958..2974)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2999..3018)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3000..3018)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3025..3043
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3167..3266
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             3529..6174
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(6670..6686)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6670..6686)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(6700..6733)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6918..6936)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7388..7459,7861..8134,8209..8863,8925..9240,9444..9575,
                     9646..10260)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(11034..11067)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   11268..11330
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11460..11477)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11460..11476)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11469..11491)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11618..12073
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11705..11724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11915..11936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc ccgccgggta actcacgggg tatccatgtc catttctgcg gcatccagcc
     2221 aggatacccg tcctcgctga cgtaatatcc cagcgccgca ccgctgtcat taatctgcac
     2281 accggcacgg cagttccggc tgtcgccggt attgttcggg ttgctgatgc gcttcgggct
     2341 gaccatccgg aactgtgtcc ggaaaagccg cgacgaactg gtatcccagg tggcctgaac
     2401 gaacagttca ccgttaaagg cgtgcatggc cacaccttcc cgaatcatca tggtaaacgt
     2461 gcgttttcgc tcaacgtcaa tgcagcagca gtcatcctcg gcaaactctt tccatgccgc
     2521 ttcaacctcg cgggaaaagg cacgggcttc ttcctccccg atgcccagat agcgccagct
     2581 tgggcgatga ctgagccgga aaaaagaccc gacgatatga tcctgatgca gctagattaa
     2641 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
     2701 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
     2761 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
     2821 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
     2881 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
     2941 atagatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc
     3001 ctatagtgag tcgtattaag atctaattaa ccctcactaa agggaacaaa agctggagct
     3061 ccaccgcggt ggcggccgct gcaggaattc ggcttcgact agtactgacg gacacaccga
     3121 agccccggcg gcaaccctca gcggatgccc cggggcttca cgttttccca ggtcagaagc
     3181 ggttttcggg agtagtgccc caactggggt aacctttgag ttctctcagt tgggggcgta
     3241 gggtcgccga catgacacaa ggggttgtga ccggggtgga cacgtacgcg ggtgcttacg
     3301 accgtcagtc gcgcgagcgc gactagtaca agccgaattc gatatcaagc ttaccgaagt
     3361 atacacttaa attcagtgca cgtttgcttg ttgagaggaa aggttgtgtg cggacgaatt
     3421 tttttttgaa aacattaacc cttacgtgga tcccgtaagc aaataaacaa gcgcagctga
     3481 acaagctaaa caatctgcag cccaagcttg aagcaagcct cctgaaagat gaagctactg
     3541 tcttctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa
     3601 aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc
     3661 aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg
     3721 gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat
     3781 tctttacagg atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa
     3841 gatgccgtca cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag
     3901 catagaataa gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg
     3961 actgtatcga ttgactcggc agctcatcat gataactcca caattccgtt ggattttatg
     4021 cccagggatg ctcttcatgg atttgattgg tctgaagagg atgacatgtc ggatggcttg
     4081 cccttcctga aaacggaccc caacaataat gggttctttg gcgacggttc tctcttatgt
     4141 attcttcgat ctattggctt taaaccggaa aattacacga actctaacgt taacaggctc
     4201 ccgaccatga ttacggatag atacacgttg gcttctagat ccacaacatc ccgtttactt
     4261 caaagttatc tcaataattt tcacccctac tgccctatcg tgcactcacc gacgctaatg
     4321 atgttgtata ataaccagat tgaaatcgcg tcgaaggatc aatggcaaat cctttttaac
     4381 tgcatattag ccattggagc ctggtgtata gagggggaat ctactgatat agatgttttt
     4441 tactatcaaa atgctaaatc tcatttgacg agcaaggtct tcgagtcagg ttccataatt
     4501 ttggtgacag ccctacatct tctgtcgcga tatacacagt ggaggcagaa aacaaatact
     4561 agctataatt ttcacagctt ttccataaga atggccatat cattgggctt gaatagggac
     4621 ctcccctcgt ccttcagtga tagcagcatt ctggaacaaa gacgccgaat ttggtggtct
     4681 gtctactctt gggagatcca attgtccctg ctttatggtc gatccatcca gctttctcag
     4741 aatacaatct ccttcccttc ttctgtcgac gatgtgcagc gtaccacaac aggtcccacc
     4801 atatatcatg gcatcattga aacagcaagg ctcttacaag ttttcacaaa aatctatgaa
     4861 ctagacaaaa cagtaactgc agaaaaaagt cctatatgtg caaaaaaatg cttgatgatt
     4921 tgtaatgaga ttgaggaggt ttcgagacag gcaccaaagt ttttacaaat ggatatttcc
     4981 accaccgctc taaccaattt gttgaaggaa cacccttggc tatcctttac aagattcgaa
     5041 ctgaagtgga aacagttgtc tcttatcatt tatgtattaa gagatttttt cactaatttt
     5101 acccagaaaa agtcacaact agaacaggat caaaatgatc atcaaagtta tgaagttaaa
     5161 cgatgctcca tcatgttaag cgatgcagca caaagaactg ttatgtctgt aagtagctat
     5221 atggacaatc ataatgtcac cccatatttt gcctggaatt gttcttatta cttgttcaat
     5281 gcagtcctag tacccataaa gactctactc tcaaactcaa aatcgaatgc tgagaataac
     5341 gagaccgcac aattattaca acaaattaac actgttctga tgctattaaa aaaactggcc
     5401 acttttaaaa tccagacttg tgaaaaatac attcaagtac tggaagaggt atgtgcgccg
     5461 tttctgttat cacagtgtgc aatcccatta ccgcatatca gttataacaa tagtaatggt
     5521 agcgccatta aaaatattgt cggttctgca actatcgccc aataccctac tcttccggag
     5581 gaaaatgtca acaatatcag tgttaaatat gtttctcctg gctcagtagg gccttcacct
     5641 gtgccattga aatcaggagc aagtttcagt gatctagtca agctgttatc taaccgtcca
     5701 ccctctcgta actctccagt gacaatacca agaagcacac cttcgcatcg ctcagtcacg
     5761 ccttttctag ggcaacagca acagctgcaa tcattagtgc cactgacccc gtctgctttg
     5821 tttggtggcg ccaattttaa tcaaagtggg aatattgctg atagctcatt gtccttcact
     5881 ttcactaaca gtagcaacgg tccgaacctc ataacaactc aaacaaattc tcaagcgctt
     5941 tcacaaccaa ttgcctcctc taacgttcat gataacttca tgaataatga aatcacggct
     6001 agtaaaattg atgatggtaa taattcaaaa ccactgtcac ctggttggac ggaccaaact
     6061 gcgtataacg cgtttggaat cactacaggg atgtttaata ccactacaat ggatgatgta
     6121 tataactatc tattcgatga tgaagatacc ccaccaaacc caaaaaaaga gtaaaatgaa
     6181 tcgtagatac tgaaaaaccc cgcaagttca cttcaactgt gcatcgtgca ccatctcaat
     6241 ttctttcatt tatacatcgt tttgccttct tttatgtaac tatactcctc taagtttcaa
     6301 tcttggccat gtaacctctg atctatagaa ttttttaaat gactagaatt aatgcccatc
     6361 ttttttttgg acctaaattc ttcatgaaaa tatattacga gggcttattc agaagcttat
     6421 cgataccgtc gactaaagcc aaatagaaaa ttattcagtt cctggcttaa gtttttaaaa
     6481 gtgatattat ttatttggtt gtaaccaacc aaaagaatgt aaataactaa tacataatta
     6541 tgttagtttt aagttagcaa caaattgatt ttagctatat tagctacttg gttaataaat
     6601 agaatatatt tatttaaaga taattgcgtt tttattgtca gggagtgagt ttgcttaaaa
     6661 actcgtttag atccactagt tctagagcgg ccgctctaga taacttcgta tagcatacat
     6721 tatacgaagt tatagcttct gatggaatta gaacttggca aaacaatact gagaatgaag
     6781 tgtatgtgga acagaggctg ctgatctcgt tcttcaggct atgaaactga cacatttgga
     6841 aaccacagta cttagaacca caaagtggga atcaagagaa aaacaatgat cccacgagag
     6901 atcgaattct agtatgtatg taagttaata aaacccattt ttgcggaaag tagataaaaa
     6961 aaacattttt tttttttact gcactggata tcattgaact tatctgatca gttttaaatt
     7021 tacttcgatc caagggtatt tgatgtacca ggttctttcg attacctctc actcaaaatg
     7081 acattccact caaagtcagc gctgtttgcc tccttctctg tccacagaaa tatcgccgtc
     7141 tctttcgccg ctgcgtccgc tatctctttc gccaccgttt gtagcgttac gtagcgtcaa
     7201 tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc
     7261 catcaattaa acacaaagtg ctgtgccaaa actcctctcg cttcttattt ttgtttgttt
     7321 tttgagtgat tggggtggtg attggttttg ggtgggtaag caggggaaag tgtgaaaaat
     7381 cccggcaatg ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc
     7441 tgccgagcat ctgaacaatg tgagtagtac atgtgcatac atcttaagtt cacttgatct
     7501 ataggaactg cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa
     7561 tcccaaaccg caattgcaca aacaaatagt gacacgaaac agattattct ggtagctgtt
     7621 ctcgctatat aagacaattt ttgagatcat atcatgatca agacatctaa aggcattcat
     7681 tttcgactat attctttttt acaaaaaata taacaaccag atattttaag ctgatcctag
     7741 atgcacaaaa aataaataaa agtataaacc tacttcgtag gatacttcgg ggtacttttt
     7801 gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag
     7861 ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac
     7921 tacggcacgc tccggccacc cagtccgccg gaggactccg gttcagggag cggccaacta
     7981 gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg
     8041 ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac
     8101 ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga
     8161 tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg
     8221 cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc
     8281 tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac
     8341 tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc
     8401 aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc caagccatgg
     8461 tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc
     8521 aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag
     8581 gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggctcta accgatccgc
     8641 cgcttctgat ctgcgatgag cccacctccg gactggactc ctttaccgcc cacagcgtcg
     8701 tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc
     8761 cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag
     8821 ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt
     8881 attaagggta tctagtatta cataacatct caactcctat ccagcgtggg tgcccagtgt
     8941 cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg
     9001 gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgccat tagcaaagta
     9061 gcccgggata tggagcagtt gttggccacc aaaaatctgg agaagccact ggagcagccg
     9121 gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga
     9181 tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg
     9241 gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc
     9301 tagattttag atagaattgc ctgattccac acccttctta gtttttttca atgagatgta
     9361 tagtttatag ttttgcagaa gataaataaa tttcatttaa ctcgcgaata ttaatgagat
     9421 gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc tttttgggcc
     9481 aacaactcac gcaagtgggt gtgatgaata tcaacggagc catcttcctc ttcctgacca
     9541 acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcatgtttag aatacatttg
     9601 catttcaata atttactaac tttctaatga atcgattcga tttaggtgtt cacctcagag
     9661 ctgccagttt ttatgaggga ggcccgaagt cgactttatc gctgtgacac atactttctg
     9721 ggcaaaacga ttgccgaatt gccgcttttt ctcacagtgc cactggtctt cacggcgatt
     9781 gcctatccga tgatcggact gcgggccgga gtgctgcact tcttcaactg cctggcgctg
     9841 gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc taatatcctg cgccagctcc
     9901 tcgacctcga tggcgctgtc tgtgggtccg ccggttatca taccattcct gctctttggc
     9961 ggcttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca
    10021 tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc
    10081 gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag
    10141 acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc cattctcatc
    10201 gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg caaggagtag
    10261 ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac catcgtgttt
    10321 actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat
    10381 atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt
    10441 gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct
    10501 acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca
    10561 taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct
    10621 ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga
    10681 ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat tagtaataaa
    10741 tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct cacagctata
    10801 tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg ttcggagtga
    10861 ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg tagatgcatc
    10921 tcaaaaaaat ggtgggcata atagtgttgt ttatatatat caaaaataac aactataata
    10981 ataagaatac atttaattta gaaaatgctt ggatttcact ggaactagaa ttcataactt
    11041 cgtatagcat acattatacg aagttatgga tcccccgggc tgcaggaatt cgataaaagt
    11101 tttgttactt tatagaagaa attttgagtt tttgtttttt ttaataaata aataaacata
    11161 aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata
    11221 tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt
    11281 tacacatgat tatctttaac gtacgtcaca atatgattat ctttctaggg ttaatctagc
    11341 tgcgtgttct gcagcgtgtc gagcatcttc atctgctcca tcacgctgta aaacacattt
    11401 gcaccgcgag tctgcccgtc ctccacgggt tcaaaaacgt gaatgaacga ggcgcgctca
    11461 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
    11521 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
    11581 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
    11641 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    11701 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    11761 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    11821 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    11881 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    11941 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    12001 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    12061 acgcttacaa tttag
//
