LOCUS       sequence-123520-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor reporting on endogenous Cdc42 activation (for 
            expression in E. coli, insect and vertebrate cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Martin K, Reimann A, Fritz RD, Ryu H, Jeon NL, Pertz O
  TITLE     Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation 
            during prototypical edge protrusion and retraction dynamics.
  JOURNAL   Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901.
  PUBMED    26912264
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:21901. doi"; pages: " 
            10.1038/srep21901"
COMMENT     Sequence Label: pTriEx4-Cdc42-2G
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             145..174
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acccaacaca atatattata gttaaataag aattattatc aaatcatttg tatattaatt
       61 aaaatactat actgtaaatt acattttatt tacaatcaaa ggagatatac catggcacac
      121 catcaccacc atcacgggag cggggagcag aaactaatat cagaggagga tcttggctca
      181 ggatcctcca ccgacggcat ggacgagctg tacaagggtg ctagcggagg aatggtgagc
      241 aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg
      301 gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg caagccctac
      361 gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc cttctcctac
      421 gacattctga ccaccgcgtt cgcctacggc aacagggcct tcaccaagta ccccgacgac
      481 atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg caccatgacc
      541 ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga ggactccttc
      601 atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt gatgcagaag
      661 aagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag
      721 ggcgacgtca agcacaagct gctgctggag ggcggcggcc acc
//
