LOCUS       sequence-123521-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor reporting on endogenous Cdc42 activation (for 
            expression in E. coli, insect and vertebrate cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Martin K, Reimann A, Fritz RD, Ryu H, Jeon NL, Pertz O
  TITLE     Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation 
            during prototypical edge protrusion and retraction dynamics.
  JOURNAL   Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901.
  PUBMED    26912264
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:21901. doi"; pages: " 
            10.1038/srep21901"
COMMENT     Sequence Label: pTriEx4-Cdc42-2G
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(18..20,21..23,24..536)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(63..84)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(324..343)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agggtggcag cggtggcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
       61 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      121 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
      181 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgatgtgc ttcgcccgct
      241 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      301 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      361 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      421 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
      481 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
      541 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgg
      601 gtaccatgca gacaattaag tgtgttgttg ttggtgatgg tgctgttggt aaaacatgtc
      661 tcctgatatc ctacacaaca aacaaattcc catcggaata tgtaccaact gtttttgaca
      721 actatgcagt cacagttatg attggtggag agccatacac tcttggactt tttgatactg
      781 cagggcaaga ggattatgac agactacgac cgctaagtta tccacagaca gatgttttt
//
