LOCUS       sequence-123523-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Fluorescent Reporter for F-actin and Nuclei.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Pertz
  TITLE     Pertz Lab_pCDNA_Lifeact-GFP_NLS-mCherry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDNA_Lifeact-GFP_NLS-mCherry
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             175..195
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(356..374)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     624..643
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
       61 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
      121 gatgataagc ttgccacaac ccgggatcca ccggtcgcta gcgccaccat gtaccccaag
      181 aagaagagaa aggtggagga ccccccggtc atggtgagca agggcgagga ggataacatg
      241 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
      301 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      361 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
      421 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
      481 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
      541 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      601 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      661 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
      721 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
      781 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
      841 gaggactaca ccatcgtgga acagtacgaa
//
