LOCUS       sequence-123526-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express EGFP-tagged FKBP for heterodimerization with FRB in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Narendra D, Kane LA, Hauser DN, Fearnley IM, Youle RJ
  TITLE     p62/SQSTM1 is required for Parkin-induced mitochondrial clustering 
            but not mitophagy; VDAC1 is dispensable for both.
  JOURNAL   Autophagy. 2010 Nov;6(8):1090-106.
  PUBMED    20890124
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2010-11"; volume: "6(8)"; pages: "1090-106"
COMMENT     Sequence Label: pC4M-F2E-GFP-FKBP
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..327
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             7..327
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             334..360
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagaggcg tgcaggtcga gaccatcagc cccggcgacg gccgcacctt tcccaagaga
       61 ggccagactt gcgtggtcca ctacaccggc atgctggagg acggcaagaa gttcgacagc
      121 agccgcgacc gcaacaagcc cttcaagttc atgctgggca aacaggaagt gatccgcggc
      181 tgggaggaag gcgtggctca gatgagcgtg gggcagcggg ccaagctgac catcagcccc
      241 gactatgcct acggcgccac cggccacccc ggcatcatcc ccccccacgc caccctggtg
      301 ttcgacgtgg agctgctgaa gctggagact agttatccgt acgacgtacc agactacgca
      361 taagaaaagt gaggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
      421 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
      481 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
      541 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
      601 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
      661 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
      721 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
      781 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt
      841 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
      901 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tc
//
