LOCUS       sequence-12353-e       12081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12081)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 12081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-attPGal4LWL
FEATURES             Location/Qualifiers
     source          1..12081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(25..55,56..609)
                     /label=P element 5' end
     primer_bind     complement(630..649)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(631..649)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        656..674
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    798..897
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             1160..3805
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(4301..4317)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4301..4317)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(4331..4364)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4549..4567)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5019..5090,5492..5765,5840..6494,6556..6871,7075..7206,
                     7277..7891)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(8665..8698)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(9113..9314,9315..9345)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(9882..9901)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10001..10023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10061..10079)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10147..10251
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10252..10320,10321..11112)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10470..10489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11283..11871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11772..11791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctggact acgtgggtct ggcccatgat gaaataacat aaggtggtcc cgtcgatagc
       61 cgaagcttac cgaagtatac acttaaattc agtgcacgtt tgcttgttga gaggaaaggt
      121 tgtgtgcgga caattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa
      181 atattgcaaa ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg
      241 tgctattaag agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca
      301 aatttgtgat acccacttta atgattcgca gtggaaggct gcacctgcaa aaggtcagac
      361 atttaaaagg aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc
      421 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
      481 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta
      541 atgaaaataa gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag
      601 agcagcagcc gaattcggta cccaattcgc cctatagtga gtcgtattaa gatctaatta
      661 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc tgcaggaatt
      721 cggcttcgac tagtactgac ggacacaccg aagccccggc ggcaaccctc agcggatgcc
      781 ccggggcttc acgttttccc aggtcagaag cggttttcgg gagtagtgcc ccaactgggg
      841 taacctttga gttctctcag ttgggggcgt agggtcgccg acatgacaca aggggttgtg
      901 accggggtgg acacgtacgc gggtgcttac gaccgtcagt cgcgcgagcg cgactagtac
      961 aagccgaatt cgatatcaag cttaccgaag tatacactta aattcagtgc acgtttgctt
     1021 gttgagagga aaggttgtgt gcggacgaat ttttttttga aaacattaac ccttacgtgg
     1081 atcccgtaag caaataaaca agcgcagctg aacaagctaa acaatctgca gcccaagctt
     1141 gaagcaagcc tcctgaaaga tgaagctact gtcttctatc gaacaagcat gcgatatttg
     1201 ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa
     1261 caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct
     1321 gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
     1381 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
     1441 aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt
     1501 ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga
     1561 agagagtagt aacaaaggtc aaagacagtt gactgtatcg attgactcgg cagctcatca
     1621 tgataactcc acaattccgt tggattttat gcccagggat gctcttcatg gatttgattg
     1681 gtctgaagag gatgacatgt cggatggctt gcccttcctg aaaacggacc ccaacaataa
     1741 tgggttcttt ggcgacggtt ctctcttatg tattcttcga tctattggct ttaaaccgga
     1801 aaattacacg aactctaacg ttaacaggct cccgaccatg attacggata gatacacgtt
     1861 ggcttctaga tccacaacat cccgtttact tcaaagttat ctcaataatt ttcaccccta
     1921 ctgccctatc gtgcactcac cgacgctaat gatgttgtat aataaccaga ttgaaatcgc
     1981 gtcgaaggat caatggcaaa tcctttttaa ctgcatatta gccattggag cctggtgtat
     2041 agagggggaa tctactgata tagatgtttt ttactatcaa aatgctaaat ctcatttgac
     2101 gagcaaggtc ttcgagtcag gttccataat tttggtgaca gccctacatc ttctgtcgcg
     2161 atatacacag tggaggcaga aaacaaatac tagctataat tttcacagct tttccataag
     2221 aatggccata tcattgggct tgaataggga cctcccctcg tccttcagtg atagcagcat
     2281 tctggaacaa agacgccgaa tttggtggtc tgtctactct tgggagatcc aattgtccct
     2341 gctttatggt cgatccatcc agctttctca gaatacaatc tccttccctt cttctgtcga
     2401 cgatgtgcag cgtaccacaa caggtcccac catatatcat ggcatcattg aaacagcaag
     2461 gctcttacaa gttttcacaa aaatctatga actagacaaa acagtaactg cagaaaaaag
     2521 tcctatatgt gcaaaaaaat gcttgatgat ttgtaatgag attgaggagg tttcgagaca
     2581 ggcaccaaag tttttacaaa tggatatttc caccaccgct ctaaccaatt tgttgaagga
     2641 acacccttgg ctatccttta caagattcga actgaagtgg aaacagttgt ctcttatcat
     2701 ttatgtatta agagattttt tcactaattt tacccagaaa aagtcacaac tagaacagga
     2761 tcaaaatgat catcaaagtt atgaagttaa acgatgctcc atcatgttaa gcgatgcagc
     2821 acaaagaact gttatgtctg taagtagcta tatggacaat cataatgtca ccccatattt
     2881 tgcctggaat tgttcttatt acttgttcaa tgcagtccta gtacccataa agactctact
     2941 ctcaaactca aaatcgaatg ctgagaataa cgagaccgca caattattac aacaaattaa
     3001 cactgttctg atgctattaa aaaaactggc cacttttaaa atccagactt gtgaaaaata
     3061 cattcaagta ctggaagagg tatgtgcgcc gtttctgtta tcacagtgtg caatcccatt
     3121 accgcatatc agttataaca atagtaatgg tagcgccatt aaaaatattg tcggttctgc
     3181 aactatcgcc caatacccta ctcttccgga ggaaaatgtc aacaatatca gtgttaaata
     3241 tgtttctcct ggctcagtag ggccttcacc tgtgccattg aaatcaggag caagtttcag
     3301 tgatctagtc aagctgttat ctaaccgtcc accctctcgt aactctccag tgacaatacc
     3361 aagaagcaca ccttcgcatc gctcagtcac gccttttcta gggcaacagc aacagctgca
     3421 atcattagtg ccactgaccc cgtctgcttt gtttggtggc gccaatttta atcaaagtgg
     3481 gaatattgct gatagctcat tgtccttcac tttcactaac agtagcaacg gtccgaacct
     3541 cataacaact caaacaaatt ctcaagcgct ttcacaacca attgcctcct ctaacgttca
     3601 tgataacttc atgaataatg aaatcacggc tagtaaaatt gatgatggta ataattcaaa
     3661 accactgtca cctggttgga cggaccaaac tgcgtataac gcgtttggaa tcactacagg
     3721 gatgtttaat accactacaa tggatgatgt atataactat ctattcgatg atgaagatac
     3781 cccaccaaac ccaaaaaaag agtaaaatga atcgtagata ctgaaaaacc ccgcaagttc
     3841 acttcaactg tgcatcgtgc accatctcaa tttctttcat ttatacatcg ttttgccttc
     3901 ttttatgtaa ctatactcct ctaagtttca atcttggcca tgtaacctct gatctataga
     3961 attttttaaa tgactagaat taatgcccat cttttttttg gacctaaatt cttcatgaaa
     4021 atatattacg agggcttatt cagaagctta tcgataccgt cgactaaagc caaatagaaa
     4081 attattcagt tcctggctta agtttttaaa agtgatatta tttatttggt tgtaaccaac
     4141 caaaagaatg taaataacta atacataatt atgttagttt taagttagca acaaattgat
     4201 tttagctata ttagctactt ggttaataaa tagaatatat ttatttaaag ataattgcgt
     4261 ttttattgtc agggagtgag tttgcttaaa aactcgttta gatccactag ttctagagcg
     4321 gccgctctag ataacttcgt atagcataca ttatacgaag ttatagcttc tgatggaatt
     4381 agaacttggc aaaacaatac tgagaatgaa gtgtatgtgg aacagaggct gctgatctcg
     4441 ttcttcaggc tatgaaactg acacatttgg aaaccacagt acttagaacc acaaagtggg
     4501 aatcaagaga aaaacaatga tcccacgaga gatcgaattc tagtatgtat gtaagttaat
     4561 aaaacccatt tttgcggaaa gtagataaaa aaaacatttt ttttttttac tgcactggat
     4621 atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat ttgatgtacc
     4681 aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag cgctgtttgc
     4741 ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt
     4801 cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat tttgtcagcg
     4861 gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt gctgtgccaa
     4921 aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt gattggtttt
     4981 gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc
     5041 tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat gtgagtagta
     5101 catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa catcaaattg
     5161 tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaattgcac aaacaaatag
     5221 tgacacgaaa cagattattc tggtagctgt tctcgctata taagacaatt tttgagatca
     5281 tatcatgatc aagacatcta aaggcattca ttttcgacta tattcttttt tacaaaaaat
     5341 ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa aagtataaac
     5401 ctacttcgta ggatacttcg gggtactttt tgttcggggt tagatgagca taacgcttgt
     5461 agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt cgcagagctg
     5521 cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac ccagtccgcc
     5581 ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg cctggcacaa
     5641 tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc tggtcaaccg
     5701 gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac atttgctcaa
     5761 gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata aagggtccaa
     5821 ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg
     5881 ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag
     5941 ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt ggacgccaag
     6001 gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg ctccctaacg
     6061 gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct gacctatcgg
     6121 cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa atgtcagcac
     6181 acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg
     6241 gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga gcccacctcc
     6301 ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag
     6361 ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga gctctttgac
     6421 aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag cgaagccgtc
     6481 gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt acataacatc
     6541 tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg cggactttta
     6601 cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa
     6661 gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt tgttggccac
     6721 caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca aggccacctg
     6781 gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca aggaaccact
     6841 cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa acaaatgata
     6901 taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg cctgattcca
     6961 cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga agataaataa
     7021 atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt gcagatggtt
     7081 gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg tgtgatgaat
     7141 atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt ctttgccacg
     7201 ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa ctttctaatg
     7261 aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg aggcccgaag
     7321 tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat tgccgctttt
     7381 tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac tgcgggccgg
     7441 agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc
     7501 cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc
     7561 gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc
     7621 agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct
     7681 gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
     7741 gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc
     7801 gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc
     7861 tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt
     7921 tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat
     7981 gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct
     8041 tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg
     8101 cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata
     8161 tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt
     8221 agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga
     8281 ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt
     8341 tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa
     8401 tatatttgta tatatttccc tcacagctat atttattcta atttaatatt atgacttttt
     8461 aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac
     8521 atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg
     8581 tttatatata tcaaaaataa caactataat aataagaata catttaattt agaaaatgct
     8641 tggatttcac tggaactaga attcataact tcgtatagca tacattatac gaagttatgg
     8701 atcctagttc tctctctctc tctcttatct atcgctactt ggttggcgcg ctctcgcgct
     8761 ctctttgtgt gcgtgtgggc agtgtgtttt ttgttgtttt gcgctttatg tgttgtattt
     8821 tgtgtgtttg gccgaagtat ttaaaacaaa agtgcagcgg aaatagttaa taacaaaata
     8881 ttagtcgacg gtagcggtcc ggttgttttc gtgctcatcg cgagtacgta tttattttta
     8941 aaaaataata aacgcggcaa acacaatcac acaaatgtgc aggcacagtg ggacaaagtg
     9001 cgctaaattt ggatatgtaa taaatccaaa accaaaaaga aaacaaaaaa cggtaaaata
     9061 ttttactgtt gttaaaattc gatcattcat tattcgctgc atgaattagc tttgcgtact
     9121 cgcaaattat taaaaataaa actttaaaaa taatttcgtc taattaatat tatgagttaa
     9181 ttcaaacccc acggacatgc taagggttaa tcaacaatca tatcgctgtc tcactcagac
     9241 tcaatacgac actcagaata ctattccttt cactcgcact tattgcaagc atacgttaag
     9301 tggatgtctc ttgccgacgg gaccacctta tgttatttca tcatggtctg gccattctca
     9361 tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt
     9421 agccgacata tatccgaaat aactgcttgt tttttttttt accattatta ccatcgtgtt
     9481 tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga
     9541 tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt
     9601 tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc
     9661 tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc
     9721 ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc
     9781 tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg
     9841 attcggtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     9901 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     9961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
    10021 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
    10081 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
    10141 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    10201 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    10261 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    10321 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    10381 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    10441 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    10501 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    10561 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    10621 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    10681 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    10741 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    10801 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    10861 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    10921 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    10981 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    11041 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    11101 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    11161 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    11221 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    11281 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    11341 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    11401 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
    11461 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    11521 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    11581 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    11641 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
    11701 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    11761 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    11821 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccttc
    11881 ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc
    11941 cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt
    12001 agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt
    12061 aacttctccg ccgccgatct g
//
