LOCUS       sequence-123531-         581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged 
            nlsTdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pTol1-uas-mAKT-2a-nlstdTomato
FEATURES             Location/Qualifiers
     source          1..581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    70..204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(107..126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     161..180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    226..246
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(339..362)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(537..556)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttttaattaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
       61 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      121 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      181 aatgtatctt atcatgtctg gatctacagg cctggcgcgc cttatcaact atgtataata
      241 aagttgttag gatcccttgt acagctcgtc catgccgtac aggaacaggt ggtggcggcc
      301 ctcggagcgc tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag
      361 cttggtgtcc acgtaataat agccgggcag ttgcacgggc ttcttggcca tgtagatggt
      421 cttgaactcc accagggagt ggccgccgtc cttcagcttc agggcctggt ggatctcgcc
      481 cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag gcctcccagc ccatggtctt
      541 cttctgcatt acggggccgt cgggggggaa gttggtgccg c
//
