LOCUS       sequence-123549-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express soluble His-tagged zebrafish erythropoietin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Stachura DL, Reyes JR, Bartunek P, Paw BH, Zon LI, Traver D
  TITLE     Zebrafish kidney stromal cell lines support multilineage 
            hematopoiesis.
  JOURNAL   Blood. 2009 Jul 9;114(2):279-89. doi: 10.1182/blood-2009-02-203638. 
            Epub 2009 May 11.
  PUBMED    19433857
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2009-02-203638"; journalName: "Blood"; date: 
            "2009-07-9- 9"; volume: "114"; issue: "2"; pages: "279-89"
COMMENT     Sequence Label: pHis-zfEpoa
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(503..520)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             537..559
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(590..609)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(591..609)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(693..712)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tctgtggatc tcagctgaca ccctgtcgac agacaggtgc attggcgagc agcagacgcg
       61 cttttccccg aagaaagttg acatggacct gaaacagctc tgagatcgag gatattttct
      121 gtgtctcctt gtcttctcca ctactggttg gaggtacata ttccggtatg ctgagacttc
      181 gcagcacttg tctgatgctg gcgatgtttc taatactggc atctatgtga gactgaagca
      241 cttcagtctg attagatatc tgtaatgagc cgatggcctc gttcagcatg tgtaagcctg
      301 actggacctc ctgagcttgc tcctctatat tcatcgcttc ccagacttca aaatcgactc
      361 tggtcaaagg aacagtgacg ttcgttgcaa tgctgcaatc gtccttacaa gttctcatag
      421 cagcctctgc atcccatgcc tccttaatga aatggtcgag gacgcgcagg tcacagatgg
      481 ggcgtaatgg ggaggatcca ccatggtgat ggtgatggtg gctagccata tgtatatctc
      541 cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc cctatagtga
      601 gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg
      661 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
      721 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
      781 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
      841 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
      901 agagcgtcga ccgatgcccc ttgaaaagcc ttcaacccag tcagctcctt ccgggtgggc
      961 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtaag
     1021 acaggtgccc gccagccgct ctgggtcatt ttc
//
