LOCUS       sequence-123550-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Stachura DL, Svoboda O, Lau RP, Balla KM, Zon LI, Bartunek P, Traver
            D
  TITLE     Clonal analysis of hematopoietic progenitor cells in the zebrafish.
  JOURNAL   Blood. 2011 Aug 4;118(5):1274-82. doi: 10.1182/blood-2011-01-331199.
            Epub 2011 Mar 17.
  PUBMED    21415264
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2011-01-331199"; journalName: "Blood"; date: 
            "2011-08-4- 4"; volume: "118"; issue: "5"; pages: "1274-82"
COMMENT     Sequence Label: pHis-zfCSF3a
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgcggtgat gccggcccga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatggctagc caccatcacc atcaccatgg agctcctgtt
      181 cctcagaaac tggacatcat gaacaaggat actattgaac aagcacacag tttaatcagc
      241 aaaactctgg aagatattcc tgcaactcat gcagcatggg taaagagcaa gagtctggct
      301 tggggcagca gcacagataa gctgcagcac ttgaaacatt acataccctc agcccctgtt
      361 ctccagaaca tcactgatat cagcagtctg gaaacctgtc ttgataaaat agtgaggggc
      421 ctgcaactac atctgaacct cctgaaagat ctcattgaag caacgaccct gtcgcagact
      481 gaccaggtga cagaactgca ggctgacatt caagaacttg tgcttcttat cgaggagctt
      541 cagaatcagt caggatttaa cccatcacag cagacatcag aagagcagtc ccagagcttc
      601 aagctcaatc tgacccaaca cctgaaaagt gatttccaga ctgaggccgc ggctcaccta
      661 atcctgcatc aactgcggga tttcagctgt gacatccttc agaggatcct cagtattcgt
      721 gtatgaccat ggtggatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg
      781 gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg
      841 agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga
      901 ctggcgatgc tgtcggaatg gacgatatcc ccgcaagaag gcccggcagt accggcataa
      961 ccaagcctat gccttacagc atccagggtg acggtgccca agaatgacaa tgagcgcatt
     1021 gttagattca tacacggggg cctgaactgc gttagc
//
