LOCUS       sequence-123552-         365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBS/U6 containing shRNA to mouse SADBs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera
            AC
  TITLE     SADB phosphorylation of gamma-tubulin regulates centrosome 
            duplication.
  JOURNAL   Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 
            2009 Aug 2.
  PUBMED    19648910
REFERENCE   2  (bases 1 to 365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1921"; 
            journalName: "Nat Cell Biol"; date: "2009-09"; volume: "11"; issue: 
            "9"; pages: "1081-92"
COMMENT     Sequence Label: pBS/U6/RNAi SadBs shRNA mouse
FEATURES             Location/Qualifiers
     source          1..365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..48
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     32..48
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        45..358
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     277..297
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tgggtacccg ctctagaact agtggatccg acgccgccat
       61 ctctaggccc gcgccggccc cctcgcacag acttgtggga gaagctcggc tactcccctg
      121 ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa
      181 gacagataat ctgttctttt taatactagc tacattttac atgataggct tggatttcta
      241 taagagatac aaatactaaa ttattatttt aaaaaacagc acaaaaggaa actcacccta
      301 actgtaaagt aattgtgtgt tttgagacta taaatatccc ttggagaaaa gccttgtttg
      361 ggaga
//
