LOCUS       sequence-123556-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 
            expression in other genetic backgrounds..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Todesco M, Balasubramanian S, Hu TT, Traw MB, Horton M, Epple P, 
            Kuhns C, Sureshkumar S, Schwartz C, Lanz C, Laitinen RA, Huang Y, 
            Chory J, Lipka V, Borevitz JO, Dangl JL, Bergelson J, Nordborg M, 
            Weigel D
  TITLE     Natural allelic variation underlying a major fitness trade-off in 
            Arabidopsis thaliana.
  JOURNAL   Nature. 2010 Jun 3;465(7298):632-6. doi: 10.1038/nature09083.
  PUBMED    20520716
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-06-3- 3"; volume: "465"; issue: "7298"; pages:
            "632-6"
COMMENT     Sequence Label: MT583
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     44..60
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     45..61
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc
       61 gataagcttg atatcgaatt cctgcagccc gagtttgtag cctattcaaa ggcattcttc
      121 gtatgaaaaa atcatcaaaa gtttattgat gaaaggagat ttttgttctc tttcaaaata
      181 aaaataaaaa gctagatatg atggtctctc tgagaagcgg tatcttggta gctttaactg
      241 gtagctcaac tcactcaacg tagtcatcga acaccgactt cacaagcttg tcgtgcgttg
      301 tttccgcatc tatttgcaca actctacacc atttatcgac ggtgttaaat acaaattctt
      361 tacccaatca tatcgagaga aggatgaaag aagaatgagg aagacggtga tgaattggaa
      421 attccgataa tgtgaaaaac tcaggtgaag aagacgagtt gaaaaaaaaa aacgtttcgt
      481 attaattcct ttgatgatga tgacatggac tgttgcatcc gacatcattt ttttaattga
      541 tgaagtggca agtaagccac caaacaaaca tgaaaatttg cctaaataaa tcagccagat
      601 aaagctatat gttagtcgca acatgaatac tattacagta attaaaaacg aaaaaacggc
      661 aagttacttc agccgtttaa tgtcgtaact caaccgacaa tctttaattc agtcgtatcg
      721 tttaataacg gccgtaattg catgctatta tagtaattat tctttcgcta ccaagtcagc
      781 tgtaactag
//
