LOCUS       sequence-123557-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 
            expression in other genetic backgrounds..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Todesco M, Balasubramanian S, Hu TT, Traw MB, Horton M, Epple P, 
            Kuhns C, Sureshkumar S, Schwartz C, Lanz C, Laitinen RA, Huang Y, 
            Chory J, Lipka V, Borevitz JO, Dangl JL, Bergelson J, Nordborg M, 
            Weigel D
  TITLE     Natural allelic variation underlying a major fitness trade-off in 
            Arabidopsis thaliana.
  JOURNAL   Nature. 2010 Jun 3;465(7298):632-6. doi: 10.1038/nature09083.
  PUBMED    20520716
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-06-3- 3"; volume: "465"; issue: "7298"; pages:
            "632-6"
COMMENT     Sequence Label: MT583
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..42
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     79..95
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     79..95
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc
       61 caccgcggtg gcggccgctc tagaactagt ggatcccccg gtgtgctcta cggtgttgcg
      121 ccacttcgcc aaaactcaca caattacatg gtggggttta gtgaagcgaa ataaagaaaa
      181 atttgtatac ataaaaaaga gagagagaga aggaaatcaa aagtaaatga gggaataaca
      241 agatgaaaga ttagtggaga tatgatcaga gattcatatc ttgatagagc cagtctcatc
      301 aaaatcgtat aaacatcaac atcgacaaaa agaagagtct acctaatcat gttgtggtca
      361 gtggttacag tgaacgaata aagagggtcc ctaagttttt gcacctcttg gaacaatcta
      421 acgtcgtttt gtcgttgcca aacgtatctt caaattttta taccccaaca aaaataataa
      481 ttcttgtgaa gtatcgtcta tattgctctt ggaaacgact catgtggaac gcacgacgag
      541 aataaaaaac aatttacctt tttttttgtt aaactcatct cctttgaatg tggttcataa
      601 attcgagaga taagtaaact ggtatctact aaaccgaata ataaaatatc actccattat
      661 tttagaggaa atttcttcga atgatatact tttaattcac gctttattac aactacagat
      721 aattctgata ggacttcgaa tgaattctat gatcaagatg ccgcttctga gagagaccag
      781 catatctcgt agctttttga aagaaaaaaa aa
//
