LOCUS       sequence-123558-       12418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 
            expression in other genetic backgrounds..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12418)
  AUTHORS   Todesco M, Balasubramanian S, Hu TT, Traw MB, Horton M, Epple P, 
            Kuhns C, Sureshkumar S, Schwartz C, Lanz C, Laitinen RA, Huang Y, 
            Chory J, Lipka V, Borevitz JO, Dangl JL, Bergelson J, Nordborg M, 
            Weigel D
  TITLE     Natural allelic variation underlying a major fitness trade-off in 
            Arabidopsis thaliana.
  JOURNAL   Nature. 2010 Jun 3;465(7298):632-6. doi: 10.1038/nature09083.
  PUBMED    20520716
REFERENCE   2  (bases 1 to 12418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-06-3- 3"; volume: "465"; issue: "7298"; pages:
            "632-6"
COMMENT     Sequence Label: MT583
FEATURES             Location/Qualifiers
     source          1..12418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(588..840)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(860..1411)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1453..1632)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1869..1891
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1883..1900
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1884..1900
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1910..1929
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1910..1928
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1953..1969
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1954..1970
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7582..7593
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9991..10007)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9991..10007)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10044..10064)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10044..10062)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10083..10099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10083..10099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10096..10118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10107..10123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10131..10137,10138..10156,10157..10162))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10177..10198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10315..10332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10437..10461
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(10552..11140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10632..10651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11311..12099)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(12390..12418,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttgcat gccggtcgat ctagtaacat
      601 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
      661 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
      721 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
      781 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
      841 tgcttgactc taggggtcat cagatttcgg tgacgggcag gaccggacgg ggcggcaccg
      901 gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg
      961 ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt
     1021 cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca
     1081 gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca
     1141 cggtggactc ggccgtccag tcgtaggcgt tgcgtgcctt ccagggaccc gcgtaggcga
     1201 tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga
     1261 cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg
     1321 tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc
     1381 ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc cctcgatcga gttgagagtg
     1441 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
     1501 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
     1561 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctcagt ggctccttca
     1621 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
     1681 cataacgtga ctcccttaat tctccgctca tgtatcgata acattaacgt ttacaatttc
     1741 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1801 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1861 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     1921 actatagggc gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga
     1981 tatcgaattc ctgcagcccg agtttgtagc ctattcaaag gcattcttcg tatgaaaaaa
     2041 tcatcaaaag tttattgatg aaaggagatt tttgttctct ttcaaaataa aaataaaaag
     2101 ctagatatga tggtctctct gagaagcggt atcttggtag ctttaactgg tagctcaact
     2161 cactcaacgt agtcatcgaa caccgacttc acaagcttgt cgtgcgttgt ttccgcatct
     2221 atttgcacaa ctctacacca tttatcgacg gtgttaaata caaattcttt acccaatcat
     2281 atcgagagaa ggatgaaaga agaatgagga agacggtgat gaattggaaa ttccgataat
     2341 gtgaaaaact caggtgaaga agacgagttg aaaaaaaaaa acgtttcgta ttaattcctt
     2401 tgatgatgat gacatggact gttgcatccg acatcatttt tttaattgat gaagtggcaa
     2461 gtaagccacc aaacaaacat gaaaatttgc ctaaataaat cagccagata aagctatatg
     2521 ttagtcgcaa catgaatact attacagtaa ttaaaaacga aaaaacggca agttacttca
     2581 gccgtttaat gtcgtaactc aaccgacaat ctttaattca gtcgtatcgt ttaataacgg
     2641 ccgtaattgc atgctattat agtaattatt ctttcgctac caagtcagct gtaactaggt
     2701 cgagttatct gttaatcaag ctcattaatc cagattgatg attttgaata aggggatatg
     2761 tcattctcac taagacgcta catcttgtag aactcatacc aagtgctatc agaattacat
     2821 ctttagttgt aagagaagaa agtacctaat taaatgatga gaatgaagag atcttcctct
     2881 aaaattatgc agccgttatt gattaacgta cagtatatgg tttagtgaat accggtttgc
     2941 ttatctctcg aattttagcc ccatacaagt aagttagatg agataaggat agtactgttt
     3001 cttttgtatt tcgatctgtc aatcagaggt tctggttccc tgagccacta gcagctctta
     3061 ttcataattt gataagacag gtcaggtgag aacgaggtaa agaaagctag gttgggaaac
     3121 tatctgtgtc gtggaaactc gtccaattat actatacaga gatagataga tagtgtgaca
     3181 aaactgatct atgattagca aaagctgaat tcggcatagt tatcctggag gatcaacgta
     3241 gcagtctaaa caactgtggt taattgtaag catcccgact tgaaaacaag cacctttttc
     3301 actaactaga gaaaaaatat tttgattaaa aacggcagag taataaacta acaaaaacag
     3361 tcacaagaga agatacattt cacggaactc gagctgtttc acgtctttta cgcttgtttg
     3421 catctgctag agatgttagc catctagtaa ttgtccatgt tttaagaaac tatatcttca
     3481 acccaaacta cttactatcg aatcattggt tcttttggta cttacatgat tcgattttag
     3541 caaacaaaat aaaacacaca cacacatgat tcgattgcta accaacacaa gaaacaaaac
     3601 aaacacatct ttgcctataa gataatgtgt ataaagcata caactagtga tgtgaaggag
     3661 gacaatgaga agactatttc attatgctga accgtccttg acgtcttagc agttaactct
     3721 gtaaaatggg ttcatatcat agtaaaagaa tcactcttac acaacaatta catgctaaaa
     3781 actgagaagc taagttacca aattctctat atagaaatac caatcactta caataaccaa
     3841 tgctgcataa caacttttga gtccataact gataaattga tagaaactgt ttgaatttgc
     3901 aaatccaaaa ccgtttctta ggatggagaa ttaatgggtt tgtatttttc tatttccgat
     3961 caaggaaaaa tatctcaggc aacaactgtg gaagatagga cattttggga tgaaggagga
     4021 aaccatagtt aacaagcgga aacaatatca aaggttgatt tcaaagatca taaagtcaaa
     4081 gtcaaatccc atagagagac tcggttacgg aagtgttggt atccgaatat ccgatagtca
     4141 tagcctcaga acttatgttg gaatcaatag gtttaaccat gcagtgtgca ctttggaaat
     4201 caagccaaga aaagctatcc acgcaaacaa tttgcgttag agcaaaatcg gataaagaga
     4261 aagaaaatat aggatttaaa aatcttatag cgagtaagac ttatttgaag catcgtcaaa
     4321 tttacttctt ttggtgtata tttctcatta tatggtgcat atatctgttt atgtaataaa
     4381 ttgtttccaa aaattactgt atactgactt tgtaatcttg ttttgatatc aatgaattta
     4441 taaggaaaaa aatataaaaa aatataaagt atgatgtaca tgtaaaaaaa gttgtttcaa
     4501 gcgtaattgt tttttggcta gagaatgaat atacagcaac agtaaactaa taaacttgcg
     4561 atgaactaaa atttctggta ttcctacaat caatgaatca ctaatttatc tataagtttt
     4621 atctatatcc gcttaaaccc cgcctcaact tgctctctgg tctgggtata gttgggctac
     4681 aacagtgaaa ccgtaattag gaaagaaatg ataaaaaccc aatccagaag cttactgcaa
     4741 gataaagaga aagatcatga agaggtagga gtgattcata taacaaacag ggtcacgttg
     4801 tcactttctc ccagaaaaat acaaatttag actaactata taaggagacg acttcagagt
     4861 cttctaatgg gttagtataa ctcgggtcat cttttaatct ctggctttaa agacatggta
     4921 agattccata tatatgaaaa ctctgtgtgt ggtggattgc ttttttcatt taaggcaaag
     4981 ataggtttta aggcagaaga caagaacgac ctttggctta tttataggag accaccactt
     5041 tcacttgagt cgagacagta acgacattta gaatttgcat tactcatctt gtccctttct
     5101 cccaggaaaa aaaaatacaa atttagacca actatattag gagacgactt caaagtcttc
     5161 taatgagtta gtaactgggg tcatctttta atcgccggct ttcaagacat gtacaatttc
     5221 atatgaaaac tctgtgtgtg gtggattgca tccaagacag ttttaagaca gaagataaga
     5281 acggcctttg cttatttata ggagaccacc actcctctcg ataaccatga ctcgagacat
     5341 taacgacttt taaaactaag ggacgaacct taagcaaaag ctcttgcatt actcaaattc
     5401 ttctgccact tggtaagtct ttttctctgc cccttctgca tgcaatggtt gacttttttg
     5461 tttgtggacg tggtagtaac tagtaaactt aaatctgtgt tttcactttt caaaccattt
     5521 gtgtgattat tttaggtttg cgatggacag ttctggagca gatctagata gaattgaggc
     5581 acaaaggtca atgttagttt ctcatgatca acggaaaggt acatgagtca tagggtggcc
     5641 acaaattttg aatattcatt acatgtgctt tattaacgag tatagtgacc ctatataaat
     5701 tattattgac acctatgcaa aaccaaccaa tctacataaa aaagataagg aaaaactatt
     5761 ctacttgatt cttgttataa actaggatat aacactagga tataactagc aatataccac
     5821 ataacaatta tattgtttta taaaaattta ataaaattag tcttattcaa catgtctttt
     5881 attaacaaaa taattaataa aaatataacc catatgaatt accggttaat aatattttaa
     5941 ctttttatta atatcgattc aaacccgttt caccatataa catgtctcac aatataactt
     6001 gtcctacatt attttaaatt gtttatatgt ttaaatatat ttataatatt ttaaactttt
     6061 taattgaatt tggatttatt aattataata ttataactta aaaaatttac tatatcaggt
     6121 aaacttaaac atttcaacta taaaaaaaac cgtatagaaa ctttgtaaat ttggatattt
     6181 actaagttaa atttcatgtt cattaagatt cgaattcatt ttttatctta ttttttttat
     6241 ggctctgaac gattgtctaa atattttagc tgatgttgga cgttgaactc aatttatttt
     6301 ctattaattg agtaatatat gttcgtttta aacaattgga atcacaagaa tacagacaaa
     6361 ttaaactttt tatgtcttat atgatgattc aaaataatta aaaaataaat cttaatgata
     6421 ggagaatcat tcttttaaat tttatgattt ttttatttta taattgatgt taaaatcttt
     6481 taattgtaaa gttgtttggt tataacttat aagtaatgac attctttgta aatactaact
     6541 gaaaacaaag ttactctcta aaatgtaatt caaaaatatt aatatagata gttaagaact
     6601 aataaatgtc actatccaag atttatataa tttagtaaaa aatctacata attttttttt
     6661 aacaaaacgc gcaagtgttt atgttctttt ctcttatgag tcttcaactt gacaaatctc
     6721 gattagttaa aatttagatt aattttgaat tgacaattag ataaagatat ttatgatttt
     6781 attaacttat tatattttga tatttacaaa tttatatttt agatttttca taagttaatt
     6841 gatttcatag ttagaactca caaattttgt ctcatgaact gtaagacgag tggttaattc
     6901 gattgatgaa ataaaacagt agaaatattt tggcaaaaag aaaacaatcg taagtgatac
     6961 aaaatagtta gaagtgataa aaattagcga taagagagct gtaaaacttt cgatatatct
     7021 acataagata gtaactacta tactagtaaa taactatttt gaccctggta acaaattgtt
     7081 ttaccagatt tctctcacag cggaggagtt ggcaccacgt cacctacggg cgacaccgaa
     7141 cctgtgccaa agttccgtac taaccttaaa ttgtcggatc ttttcgctct ccctggtgaa
     7201 gatgtcgaga tgactccgga gatttttggt ggaatgagta atggggaaaa agaatgtctg
     7261 gagaagttga gaagcaatgg aacgccaatg gaacgtgtca agagcaatac aggagattca
     7321 attcttcata ttgctgctaa atggggtcat ctggaactag tgaaggagat tgtctttgaa
     7381 tgtccatgcc ttctattcga gcaaaactca agccgtcaga caccgctaca tgtggctgct
     7441 catggcggtc ataccaaagt tgttgaggct cttgttgcat cggtaacatc tgcttcagct
     7501 agtctgagta ctgaagagag tgagagactg aatccacatg ttcgtaagga cgaagatgga
     7561 aatactgctc tgtactacgc cattgaagga cgctatttgg agatggctac ctgtctggtg
     7621 aacgcggaca aggatgctcc ttttcttgga aataacaagg gaatatcttc cttgtacgaa
     7681 gccgtagacg ctggaaataa atttgaagac cttgtgaaag caattttgaa aaccacagac
     7741 gacaacgtgg accgtgaagt gaggaagttt aacttagatt cgaaattaca aggaaacaaa
     7801 catcttgcac atgttgcttt gaaggccaaa agtataggtt tgttttttgt tttctttaat
     7861 aatcagtaac agctacgttt aatctgttaa aattatgtaa aaccagcttg ggacgagaat
     7921 ctcctcccca agatggctaa ttgttgttcc caattgtttg agtaggtgtc cttgatgtta
     7981 ttcttgatga atatccaagt cttatggacg agcaagatga agatggtagg acttgtcttt
     8041 catacggagc atccattggg tattataaag gactatgcaa catcttaaac cgatcaacaa
     8101 agggtgttta tgtctgcgac caagatggtt cttttccaat tcattcggct gcaaaaaacg
     8161 atcattacga aattattaaa gagtttataa aacgttgtcc agcttcaaaa taccttctta
     8221 acagacttgg tcagaacatt ctccacgtgg cggcaaaaaa tgaggcctct cttactgcat
     8281 atatgttgat gcttgataaa gatacgaaac atctgggtgt tgggcaagat gtggacggga
     8341 atacaccttt gcaccttgcc gtcatgaact gggactttga ctccattact tgtcttgcta
     8401 gccgtaacca cgagatactg aaattacgaa acaaaagcgg cttaagagct agggatattg
     8461 ccgagtcaga ggtgaaaccc aactacatct ttcatgaggt atttctgatt ccgtttcata
     8521 caacacattt gatccctcat aaatgattta tagcttgacc tccatatttt tggaattaca
     8581 gaggtggaca ctggcgctct tattatacgc tattcactca agtggtttcg aatctgttaa
     8641 gtcattaaca aaacagtcag tgccactaga ccctaagaat aacagacatt acgtcaacgc
     8701 tcttctcgtg gtagcggctc ttgtagccac agtgacgttt gctgcaggct ttacaatacc
     8761 aggtgggtat atcagcgata gcaaaaaacc aaacttgggc agggcaactt tggccactaa
     8821 cccaactctc ttcatttttc tgctttttga catcttggca atgcaaagtt ctgttgcaac
     8881 aatatgtact cttatttggg cgcagttagg tgatccaaac ctcatccgca aatccttaca
     8941 tgtggccttg cccttactac ttttttcatt actatgcatg cccgtggcat tcctttttgg
     9001 cgtgatcact gcaattgccc atgtgaaatg gcttttagtc accattagca ttatatctgg
     9061 tggattcttc ctttgcgcaa tctttatcct tggccctcac gtcatgctac agcggtcata
     9121 ctttccgccc agtgctggta tatatctcag gacttttatg ctgactatag acatatctga
     9181 gttttttgtg cgtaagatca aaacttgttt tggttgtgtg gcgtgtgaat aaatcatcaa
     9241 aagtttatag ttcaaaggat atgtttcttc tctttttttt ttctttcaaa aagctacgag
     9301 atatgctggt ctctctcaga agcggcatct tgatcataga attcattcga agtcctatca
     9361 gaattatctg tagttgtaat aaagcgtgaa ttaaaagtat atcattcgaa gaaatttcct
     9421 ctaaaataat ggagtgatat tttattattc ggtttagtag ataccagttt acttatctct
     9481 cgaatttatg aaccacattc aaaggagatg agtttaacaa aaaaaaaggt aaattgtttt
     9541 ttattctcgt cgtgcgttcc acatgagtcg tttccaagag caatatagac gatacttcac
     9601 aagaattatt atttttgttg gggtataaaa atttgaagat acgtttggca acgacaaaac
     9661 gacgttagat tgttccaaga ggtgcaaaaa cttagggacc ctctttattc gttcactgta
     9721 accactgacc acaacatgat taggtagact cttctttttg tcgatgttga tgtttatacg
     9781 attttgatga gactggctct atcaagatat gaatctctga tcatatctcc actaatcttt
     9841 catcttgtta ttccctcatt tacttttgat ttccttctct ctctctcttt tttatgtata
     9901 caaatttttc tttatttcgc ttcactaaac cccaccatgt aattgtgtga gttttggcga
     9961 agtggcgcaa caccgtagag cacaccgggg gatccactag ttctagagcg gccgccaccg
    10021 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
    10081 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    10141 gccggaaghc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10201 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10261 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10321 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatga aggccttgac
    10441 aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc tcatgtgagc
    10501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10861 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10921 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10981 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaag
    11041 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11101 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11161 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    11221 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    11281 aagtatatat gtgtaacatt ggtctagtga ttatttgccg actaccttgg tgatctcgcc
    11341 tttcacgtag tgaacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttg
    11401 tccaagataa gcctgcctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
    11461 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
    11521 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
    11581 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
    11641 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
    11701 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
    11761 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
    11821 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
    11881 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
    11941 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
    12001 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
    12061 tagttgagtc gatacttcgg cgatcaccgc ttccctcata acaccccttg tattactgtt
    12121 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
    12181 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
    12241 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
    12301 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg
    12361 gctggatgat ggggcgattc aggcgatccc catccaacag cccgccgtcg agcgggct
//
