LOCUS       sequence-12356-e        9650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-GAL4DBD
FEATURES             Location/Qualifiers
     source          1..9650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(25..55,56..609)
                     /label=P element 5' end
     protein_bind    complement(623..656)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    701..770
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     961..977
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1286..1726
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    complement(2028..2061)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2246..2264)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2716..2787,3189..3462,3537..4191,4253..4568,4772..4903,
                     4974..5588)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(6362..6395)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6419..6452)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(6682..6883,6884..6914)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(7451..7470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7570..7592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7630..7648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7716..7820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7821..7889,7890..8681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8039..8058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8852..9440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9341..9360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctggact acgtgggtct ggcccatgat gaaataacat aaggtggtcc cgtcgatagc
       61 cgaagcttac cgaagtatac acttaaattc agtgcacgtt tgcttgttga gaggaaaggt
      121 tgtgtgcgga caattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa
      181 atattgcaaa ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg
      241 tgctattaag agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca
      301 aatttgtgat acccacttta atgattcgca gtggaaggct gcacctgcaa aaggtcagac
      361 atttaaaagg aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc
      421 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
      481 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta
      541 atgaaaataa gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag
      601 agcagcagcc gaattcggta ccgaagttcc tatactttct agagaatagg aacttcggcg
      661 cgccagtacc gggccccccc tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
      721 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
      781 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
      841 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
      901 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
      961 cgaggtcgac ggtatcgata agcttaccga agtatacact taaattcagt gcacgtttgc
     1021 ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt
     1081 ggatccgcgg ccgcaaaaca tgctggagat ccgcgccgcc ttcctgcgcc agcgcaacac
     1141 cgccctgcgc accgaggtgg ccgagctgga gcaggaggtg cagcgcctgg agaacgaggt
     1201 gagccagtac gagacccgct acggccccct gggcggcggc aaggctagcg gaggaggtgg
     1261 tggaggtgga ggtggaggta ctagtatgaa gctactgtct tctatcgaac aagcatgcga
     1321 tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct
     1381 gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc
     1441 acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt
     1501 tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt
     1561 gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag atagattggc
     1621 ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg cgacatcatc
     1681 atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgtaag gcgcgcctct
     1741 agatcgatac cgtcgactaa agccaaatag aaaattattc agttcctggc ttaagttttt
     1801 aaaagtgata ttatttattt ggttgtaacc aaccaaaaga atgtaaataa ctaatacata
     1861 attatgttag ttttaagtta gcaacaaatt gattttagct atattagcta cttggttaat
     1921 aaatagaata tatttattta aagataattg cgtttttatt gtcagggagt gagtttgctt
     1981 aaaaactcgt ttagatccac tagcttaatt aagcatgcct aggctagata acttcgtata
     2041 gcatacatta tacgaagtta tagcttctga tggaattaga acttggcaaa acaatactga
     2101 gaatgaagtg tatgtggaac agaggctgct gatctcgttc ttcaggctat gaaactgaca
     2161 catttggaaa ccacagtact tagaaccaca aagtgggaat caagagaaaa acaatgatcc
     2221 cacgagagat cgaattctag tatgtatgta agttaataaa acccattttt gcggaaagta
     2281 gataaaaaaa acattttttt tttttactgc actggatatc attgaactta tctgatcagt
     2341 tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat tacctctcac
     2401 tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc cacagaaata
     2461 tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt agcgttacgt
     2521 agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa gctccaagcg
     2581 gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct tcttattttt
     2641 gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca ggggaaagtg
     2701 tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg aggcagcaaa
     2761 cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat cttaagttca
     2821 cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag aactgcgacc
     2881 cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag attattctgg
     2941 tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag acatctaaag
     3001 gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat attttaagct
     3061 gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga tacttcgggg
     3121 tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga gatcccctat
     3181 cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc ttcgggcagg
     3241 ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt tcagggagcg
     3301 gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt ggggcggtca
     3361 atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta ttctgcaacg
     3421 agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt ctattcgcag
     3481 tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa actcagtttg
     3541 cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg ccggaaagac
     3601 gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat cgccatccgg
     3661 gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca ggtgcgccta
     3721 tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc tgattttcca
     3781 agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc gcgtggatca
     3841 ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg tgcccggcag
     3901 ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg aggctctaac
     3961 cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct ttaccgccca
     4021 cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca tcctgaccat
     4081 tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga tggccgaggg
     4141 cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct agtgagttcg
     4201 atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc agcgtgggtg
     4261 cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg gccgttgtgc
     4321 ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat tttgccatta
     4381 gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag aagccactgg
     4441 agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc cgggcggtcc
     4501 tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg cgacttattc
     4561 agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat tcttggaaac
     4621 aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt ttttttcaat
     4681 gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact cgcgaatatt
     4741 aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg gcctcatctt
     4801 tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca tcttcctctt
     4861 cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc atgtttagaa
     4921 tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt taggtgttca
     4981 cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc tgtgacacat
     5041 actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca ctggtcttca
     5101 cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc ttcaactgcc
     5161 tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta atatcctgcg
     5221 ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata ccattcctgc
     5281 tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt
     5341 acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg
     5401 agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca
     5461 tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggcca
     5521 ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca
     5581 aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc attattacca
     5641 tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa
     5701 aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca caagtagact
     5761 ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt
     5821 ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc
     5881 cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca
     5941 cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt
     6001 ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta
     6061 gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat atttccctca
     6121 cagctatatt tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt
     6181 cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta
     6241 gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa
     6301 ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg aactagaatt
     6361 cataacttcg tatagcatac attatacgaa gttatggatc tggccgcgct cttccgctga
     6421 agttcctata ctttctagag aataggaact tcctcgacgg tagcggtccg gttgttttcg
     6481 tgctcatcgc gagtacgtat ttatttttaa aaaataataa acgcggcaaa cacaatcaca
     6541 caaatgtgca ggcacagtgg gacaaagtgc gctaaatttg gatatgtaat aaatccaaaa
     6601 ccaaaaagaa aacaaaaaac ggtaaaatat tttactgttg ttaaaattcg atcattcatt
     6661 attcgctgca tgaattagct ttgcgtactc gcaaattatt aaaaataaaa ctttaaaaat
     6721 aatttcgtct aattaatatt atgagttaat tcaaacccca cggacatgct aagggttaat
     6781 caacaatcat atcgctgtct cactcagact caatacgaca ctcagaatac tattcctttc
     6841 actcgcactt attgcaagca tacgttaagt ggatgtctct tgccgacggg accaccttat
     6901 gttatttcat catggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc
     6961 tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt
     7021 ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg
     7081 taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt
     7141 cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc
     7201 ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat
     7261 acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta
     7321 gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac
     7381 cttggagcga ctgtcgttag ttccgcgcga ttcggtgcgg tatttcacac cgcatatggt
     7441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     7501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     7561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     7621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     9001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9241 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9421 gggggcggag cctatggaaa aacgccttct tcttgaactc gggctcggtg ccagtatacc
     9481 tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc
     9541 aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca
     9601 gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg
//
