LOCUS       sequence-123563-         431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Murakoshi H, Lee SJ, Yasuda R
  TITLE     Highly sensitive and quantitative FRET-FLIM imaging in single 
            dendritic spines using improved non-radiative YFP.
  JOURNAL   Brain Cell Biol. 2008 Aug . 36(1-4):31-42.
  PUBMED    18512154
REFERENCE   2  (bases 1 to 431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Brain Cell 
            Biol. 2008 Aug . 36(1-4):31-42."
COMMENT     Sequence Label: pCAG-mGFP-Actin
FEATURES             Location/Qualifiers
     source          1..431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(63..82)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(148..170)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtagcg ctagccaatt ctttgccaaa atgatgagac
       61 agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg
      121 ttagcagagg ctctagctag agccgccggt cacacgccag aagccgaacc ccgccctgcc
      181 ccgtcccccc cgaaggcagc cgtccccccg cggacagccc cgaggctgga gagggagaag
      241 gggacggcgg cgcggcgacg cacgaaggcc ctccccgccc atttccttcc tgccggcgcc
      301 gcaccgcttc gccccgcgcc cgctagaggg ggtgcggcgg cgcctcccag atttcggctc
      361 cgcacagatt tgggacaaag gaagtccctg cgccctctcg cacgattacc ataaaaggca
      421 atggctgcgg c
//
