LOCUS       sequence-12357-e       10318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-GAL80
FEATURES             Location/Qualifiers
     source          1..10318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(25..55,56..609)
                     /label=P element 5' end
     protein_bind    complement(623..656)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    701..770
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     961..977
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1098..2405
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL80"
                     /product="transcriptional repressor that inhibits GAL4"
                     /label=GAL80
                     /note="GAL80 binds to and inhibits the transcriptional 
                     activation domain of GAL4."
                     /translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSS
                     QFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEVVM
                     PLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKE
                     LISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMT
                     SSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKG
                     GKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLD
                     PGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGF
                     PTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
     protein_bind    complement(2696..2729)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2914..2932)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3384..3455,3857..4130,4205..4859,4921..5236,5440..5571,
                     5642..6256)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(7030..7063)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7087..7120)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(7350..7551,7552..7582)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(8119..8138)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8238..8260
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8298..8316)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8384..8488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8489..8557,8558..9349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8707..8726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9520..10108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10009..10028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctggact acgtgggtct ggcccatgat gaaataacat aaggtggtcc cgtcgatagc
       61 cgaagcttac cgaagtatac acttaaattc agtgcacgtt tgcttgttga gaggaaaggt
      121 tgtgtgcgga caattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa
      181 atattgcaaa ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg
      241 tgctattaag agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca
      301 aatttgtgat acccacttta atgattcgca gtggaaggct gcacctgcaa aaggtcagac
      361 atttaaaagg aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc
      421 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
      481 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta
      541 atgaaaataa gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag
      601 agcagcagcc gaattcggta ccgaagttcc tatactttct agagaatagg aacttcggcg
      661 cgccagtacc gggccccccc tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
      721 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
      781 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
      841 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
      901 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
      961 cgaggtcgac ggtatcgata agcttaccga agtatacact taaattcagt gcacgtttgc
     1021 ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt
     1081 ggatccgcgg ccgcaacatg gactacaaca agagatcttc ggtctcaacc gtgcctaatg
     1141 cagctcccat aagagtcgga ttcgtcggtc tcaacgcagc caaaggatgg gcaatcaaga
     1201 cacattaccc cgccatactg caactatcgt cacaatttca aatcactgcc ttatacagtc
     1261 caaaaattga gacttctatt gccaccattc agcgtctaaa attgagtaat gccactgctt
     1321 ttcccacttt agagtcattt gcatcatctt ccactataga tatgatagtg atagctatcc
     1381 aagtggccag ccattatgaa gttgttatgc ctctcttgga attctccaaa aataatccga
     1441 acctcaagta tcttttcgta gaatgggccc ttgcatgttc actagatcaa gccgaatcca
     1501 tttataaggc tgctgctgaa cgtggggttc aaaccatcat ctctttacaa ggtcgtaaat
     1561 caccatatat tttgagagca aaagaattaa tatctcaagg ctatatcggc gacattaatt
     1621 cgatcgagat tgctggaaat ggcggttggt acggctacga aaggcctgtt aaatcaccaa
     1681 aatacatcta tgaaatcggg aacggtgtag atctggtaac cacaacattt ggtcacacaa
     1741 tcgatatttt acaatacatg acaagttcgt acttttccag gataaatgca atggttttca
     1801 ataatattcc agagcaagag ctgatagatg agcgtggtaa ccgattgggc cagcgagtcc
     1861 caaagacagt accggatcat cttttattcc aaggcacatt gttaaatggc aatgttccag
     1921 tgtcatgcag tttcaaaggt ggcaaaccta ccaaaaaatt taccaaaaat ttggtcattg
     1981 acattcacgg taccaaggga gatttgaaac ttgaaggcga tgccggcttc gcagaaattt
     2041 caaatctggt cctttactac agtggaacta gagcaaacga cttcccgcta gccaatggac
     2101 aacaagctcc tttagacccg gggtatgatg caggtaaaga aatcatggaa gtatatcatt
     2161 tacgaaatta taatgccatt gtgggtaata ttcatcgact gtatcaatct atctctgact
     2221 tccacttcaa tacaaagaaa attcctgaat taccctcaca atttgtaatg caaggtttcg
     2281 atttcgaagg ctttcccacc ttgatggatg ctctgatatt acacaggtta atcgagagcg
     2341 tttataaaag taacatgatg ggctccacat taaacgttag caatatctcg cattatagtt
     2401 tataatctag atcgataccg tcgactaaag ccaaatagaa aattattcag ttcctggctt
     2461 aagtttttaa aagtgatatt atttatttgg ttgtaaccaa ccaaaagaat gtaaataact
     2521 aatacataat tatgttagtt ttaagttagc aacaaattga ttttagctat attagctact
     2581 tggttaataa atagaatata tttatttaaa gataattgcg tttttattgt cagggagtga
     2641 gtttgcttaa aaactcgttt agatccacta gcttaattaa gcatgcctag gctagataac
     2701 ttcgtatagc atacattata cgaagttata gcttctgatg gaattagaac ttggcaaaac
     2761 aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctt caggctatga
     2821 aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca agagaaaaac
     2881 aatgatccca cgagagatcg aattctagta tgtatgtaag ttaataaaac ccatttttgc
     2941 ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat tgaacttatc
     3001 tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt ctttcgatta
     3061 cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct tctctgtcca
     3121 cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag
     3181 cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc gtgacgaagc
     3241 tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc ctctcgcttc
     3301 ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg ggtaagcagg
     3361 ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta attcgcggag
     3421 gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt gcatacatct
     3481 taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa
     3541 ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat
     3601 tattctggta gctgttctcg ctatataaga caatttttga gatcatatca tgatcaagac
     3661 atctaaaggc attcattttc gactatattc ttttttacaa aaaatataac aaccagatat
     3721 tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact tcgtaggata
     3781 cttcggggta ctttttgttc ggggttagat gagcataacg cttgtagttg atatttgaga
     3841 tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta accagggctt
     3901 cgggcaggcc aaaaactacg gcacgctccg gccacccagt ccgccggagg actccggttc
     3961 agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg acatctttgg
     4021 ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac gcggactatt
     4081 ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg gtgagtttct
     4141 attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc aatttgaaac
     4201 tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag ttccggtgcc
     4261 ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg
     4321 ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat gcaggccagg
     4381 tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag ggaacacctg
     4441 attttccaag ccatggtgcg gatgccacga catctgacct atcggcagcg agtggcccgc
     4501 gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat catcggtgtg
     4561 cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt cgcctccgag
     4621 gctctaaccg atccgccgct tctgatctgc gatgagccca cctccggact ggactccttt
     4681 accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc
     4741 ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat ccttctgatg
     4801 gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt cttttcctag
     4861 tgagttcgat gtgtttatta agggtatcta gtattacata acatctcaac tcctatccag
     4921 cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac aggtgttggc
     4981 cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat gcgacaattt
     5041 tgccattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa atctggagaa
     5101 gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca tgcagttccg
     5161 ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg
     5221 acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg cttacaattc
     5281 ttggaaacaa attcgctaga ttttagatag aattgcctga ttccacaccc ttcttagttt
     5341 ttttcaatga gatgtatagt ttatagtttt gcagaagata aataaatttc atttaactcg
     5401 cgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc
     5461 ctcatctttt tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa cggagccatc
     5521 ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtcat
     5581 gtttagaata catttgcatt tcaataattt actaactttc taatgaatcg attcgattta
     5641 ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg
     5701 tgacacatac tttctgggca aaacgattgc cgaattgccg ctttttctca cagtgccact
     5761 ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt
     5821 caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat
     5881 atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc
     5941 attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg
     6001 gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc
     6061 ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg
     6121 caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg
     6181 tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc
     6241 ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat
     6301 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
     6361 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
     6421 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
     6481 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
     6541 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
     6601 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
     6661 cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat
     6721 agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat
     6781 ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt aattttttgt
     6841 gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc
     6901 cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa
     6961 aataacaact ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa
     7021 ctagaattca taacttcgta tagcatacat tatacgaagt tatggatctg gccgcgctct
     7081 tccgctgaag ttcctatact ttctagagaa taggaacttc ctcgacggta gcggtccggt
     7141 tgttttcgtg ctcatcgcga gtacgtattt atttttaaaa aataataaac gcggcaaaca
     7201 caatcacaca aatgtgcagg cacagtggga caaagtgcgc taaatttgga tatgtaataa
     7261 atccaaaacc aaaaagaaaa caaaaaacgg taaaatattt tactgttgtt aaaattcgat
     7321 cattcattat tcgctgcatg aattagcttt gcgtactcgc aaattattaa aaataaaact
     7381 ttaaaaataa tttcgtctaa ttaatattat gagttaattc aaaccccacg gacatgctaa
     7441 gggttaatca acaatcatat cgctgtctca ctcagactca atacgacact cagaatacta
     7501 ttcctttcac tcgcacttat tgcaagcata cgttaagtgg atgtctcttg ccgacgggac
     7561 caccttatgt tatttcatca tggtctggcc attctcatcg tgagcttccg ggtgctcgca
     7621 tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac
     7681 tgcttgtttt tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa
     7741 agctaatgta attatatttg tgccaataaa aacaagatat gacctataga atacaagtat
     7801 ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac
     7861 acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt
     7921 ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac
     7981 gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt
     8041 tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggtgcggta tttcacaccg
     8101 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     8161 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     8221 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     8281 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     8341 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8401 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8461 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8521 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8581 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8641 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8701 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     8761 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8821 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8881 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8941 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9001 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9061 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9121 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9181 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9241 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9301 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9361 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9421 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9481 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9541 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9601 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9661 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9721 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9781 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9841 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9901 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9961 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10021 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10081 ctcgtcaggg gggcggagcc tatggaaaaa cgccttcttc ttgaactcgg gctcggtgcc
    10141 agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct
    10201 gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
    10261 gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctg
//
