LOCUS       sequence-123570-        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aldehyde dehydrogenase from Escherichia coli, codon 
            optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  TITLE     Siegel Lab ALDH Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ecALDH
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..93
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        74..92
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    93..117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..154
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc
       61 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
      121 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgaacttc caccatctgg
      181 catattggca ggataaagca ctgagcctgg caattgaaaa tcgcctgttt attaacggtg
      241 aatataccgc agcagcagaa aacgaaacct ttgaaaccgt tgatccggtt acccaggcac
      301 cgctggcaaa aattgcacgt ggtaaaagcg ttgatattga tcgtgcaatg agcgcagcac
      361 gtggtgtttt tgaacgtggt gattggagcc tgagcagtcc ggcaaaacgt aaagcagttc
      421 tgaataaact ggccgatctg atggaagcac atgcagaaga actggcactg ctggaaaccc
      481 tggataccgg caaaccgatt cgtcatagcc tgcgtgatga tattccgggt gcagcccgtg
      541 caattcgttg gtatgcagaa gcaattgata aagtctatgg tgaagttgca accaccagta
      601 gccatgaact ggcaatgatt gttcgtgaac ctgttggtgt tattgcagca attgttccgt
      661 ggaattttcc actgctgctg acctgttgga aactgggtcc ggcactggca gcaggtaata
      721 gcgttattct gaaaccgagc gaaaaaagtc cgctgagcgc aattcgtctg gcaggtctgg
      781 caaaagaagc aggtctgccg gacggtgttc tgaatgttgt taccggcttt ggtcatgaag
      841 ccggtcaggc cctgagccgt cataacgata ttgatgcaat tgcctttacc ggcagcaccc
      901 gtacaggtaa acagctgctg aaagatgccg gtgatagcaa tatgaaacgt gtttggctgg
      961 aagccggtgg taaatcagca aatattgttt ttgcagattg tccggatctg caacaggcag
     1021 caagcgcaac cgcagccggt attttctata atcagggtca ggtttgtatt gcaggcacac
     1081 gcctgctgct ggaagaaagc attgcagatg aatttctggc cctgctgaaa cagcaggc
//
