LOCUS       sequence-123575-        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of CHD5 histone binding domain (331-672aa) with
            Y619 mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Egan CM, Nyman U, Skotte J, Streubel G, Turner S, O'Connell DJ, 
            Rraklli V, Dolan MJ, Chadderton N, Hansen K, Farrar GJ, Helin K, 
            Holmberg J, Bracken AP
  TITLE     CHD5 is required for neurogenesis and has a dual role in 
            facilitating gene expression and polycomb gene repression.
  JOURNAL   Dev Cell. 2013 Aug 12;26(3):223-36. doi: 
            10.1016/j.devcel.2013.07.008.
  PUBMED    23948251
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.07"; journalName: "Dev Cell"; date: 
            "2013-08-12- 12"; volume: "26"; issue: "3"; pages: "223-36"
COMMENT     Sequence Label: pCMV-FLAG-CHD5-Y619E-FRAG
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(189..983)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(300..319)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     910..929
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gctgcaataa cagttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
       61 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
      121 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
      181 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
      241 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
      301 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
      361 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
      421 tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg
      481 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
      541 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
      601 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
      661 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
      721 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
      781 gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca
      841 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
      901 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
      961 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcaaatccg
     1021 aaaatggata tacaagctcc cgggaacttt ttgcaaaaag ccttaggcct ccaaaaaagc
     1081 cctccttccc tacttctgga atag
//
