LOCUS       sequence-123576-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG inducible bacterial expression of CHD5 fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Egan CM, Nyman U, Skotte J, Streubel G, Turner S, O'Connell DJ, 
            Rraklli V, Dolan MJ, Chadderton N, Hansen K, Farrar GJ, Helin K, 
            Holmberg J, Bracken AP
  TITLE     CHD5 is required for neurogenesis and has a dual role in 
            facilitating gene expression and polycomb gene repression.
  JOURNAL   Dev Cell. 2013 Aug 12;26(3):223-36. doi: 
            10.1016/j.devcel.2013.07.008.
  PUBMED    23948251
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.07"; journalName: "Dev Cell"; date: 
            "2013-08-12- 12"; volume: "26"; issue: "3"; pages: "223-36"
COMMENT     Sequence Label: pGEX-GST-CHD5-WT-FRAG
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(201..305)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     373..391
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     536..558
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    648..672
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcattggaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
       61 gttcgatgtt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
      121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
      181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
      241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
      301 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
      361 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt atacactccg
      421 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
      481 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
      541 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gatcgtcagt
      601 cagtcacgat gcggccgctc gagtcgaccc gggaattccg gggatctacc actttgtaca
      661 agaaagctgg gtcgaattcg cccttctact gcttgtcgtc cctcagcttc ttgcccttct
      721 tgagcagcct cttgggcagc ctggtgtctt ctcccagcat cagctccctg tggccccagt
      781 aggcctgctt gaggttgtcg tagtagggga tgtcgatgtc atcgatctcc caggtgcact
      841 ggtcgtaggg caggtctttc cacttgatca ggtagtgcac atcccccttc ttgtcaaagc
      901 tatggttcag gattcggtga atcatcatcc actctggctt gatgccatag cggtagaagc
      961 gctcctccat cttggcatag agggggtcct tgttcttcct cttctcgctc ttgccgtctt
     1021 catccccaga gccgtagtca aaggggggcg gctcatccat gt
//
