LOCUS       sequence-123577-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient mammalian expression of CHD5 for BirA tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Egan CM, Nyman U, Skotte J, Streubel G, Turner S, O'Connell DJ, 
            Rraklli V, Dolan MJ, Chadderton N, Hansen K, Farrar GJ, Helin K, 
            Holmberg J, Bracken AP
  TITLE     CHD5 is required for neurogenesis and has a dual role in 
            facilitating gene expression and polycomb gene repression.
  JOURNAL   Dev Cell. 2013 Aug 12;26(3):223-36. doi: 
            10.1016/j.devcel.2013.07.008.
  PUBMED    23948251
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.07"; journalName: "Dev Cell"; date: 
            "2013-08-12- 12"; volume: "26"; issue: "3"; pages: "223-36"
COMMENT     Sequence Label: pCAG-Flag-Avi-ires-CHD5
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    43..267
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     256..273
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(469..489)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 tgattcgcca gcttgggctg caggttcttt ccgcctcaga agccatagag cccaccgcat
       61 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
      121 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
      181 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
      241 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgagctcta gatcatcgat
      301 gcatccagat ctccatcgat ggagatctgg atgcatgggg tcgtgcgctc ctttcggtcg
      361 ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg caccaggtgc
      421 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg
      481 ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct
      541 ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc
      601 cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca
      661 tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg
      721 cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt
      781 cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaaggaa gaagttcttg
      841 cagctcgggt gaaccgctcg aatgtgggcg gtccggatcg aacgttgtgg cgcgtggcgg
      901 gggtaattcc gccaaacgcg gcggcgaagg ttgcgtacgg ccttggggaa agtccgtccc
      961 gggttggcga ag
//
