LOCUS       sequence-12358-e       10476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10476)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 10476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-LexA
FEATURES             Location/Qualifiers
     source          1..10476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(25..55,56..609)
                     /label=P element 5' end
     protein_bind    complement(623..656)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    701..770
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     961..977
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1082..1098)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1082..1098)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(1163..1414,1415..1768)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     1706..1727
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             1814..2047
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(2199..2218)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2243..2291
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(2854..2887)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3072..3090)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3542..3613,4015..4288,4363..5017,5079..5394,5598..5729,
                     5800..6414)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(7188..7221)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7245..7278)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(7508..7709,7710..7740)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(8277..8296)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8396..8418
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8456..8474)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8542..8646
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8647..8715,8716..9507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8865..8884)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9678..10266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10167..10186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctggact acgtgggtct ggcccatgat gaaataacat aaggtggtcc cgtcgatagc
       61 cgaagcttac cgaagtatac acttaaattc agtgcacgtt tgcttgttga gaggaaaggt
      121 tgtgtgcgga caattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa
      181 atattgcaaa ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg
      241 tgctattaag agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca
      301 aatttgtgat acccacttta atgattcgca gtggaaggct gcacctgcaa aaggtcagac
      361 atttaaaagg aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc
      421 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
      481 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta
      541 atgaaaataa gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag
      601 agcagcagcc gaattcggta ccgaagttcc tatactttct agagaatagg aacttcggcg
      661 cgccagtacc gggccccccc tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
      721 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
      781 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
      841 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
      901 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
      961 cgaggtcgac ggtatcgata agcttaccga agtatacact taaattcagt gcacgtttgc
     1021 ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt
     1081 ggatccacta gttctagagc ggccgcgcat ggtaccgggc cccccctcga gctcgcgaaa
     1141 gcttgaatta attccgggcg gaatgaaagc gttaacggcc aggcaacaag aggtgtttga
     1201 tctcatccgt gatcacatca gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca
     1261 gcgtttgggg ttccgttccc caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa
     1321 aggcgttatt gaaattgttt ccggcgcatc acgcgggatt cgtctgttgc aggaagagga
     1381 agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa ccacttctgg cgcaacagca
     1441 tattgaaggt cattatcagg tcgatccttc cttattcaag ccgaatgctg atttcctgct
     1501 gcgcgtcagc gggatgtcga tgaaagatat cggcattatg gatggtgact tgctggcagt
     1561 gcataaaact caggatgtac gtaacggtca ggtcgttgtc gcacgtattg atgacgaggt
     1621 taccgttaag cgcctgaaaa aacagggcaa taaagtcgaa ctgttgccag aaaatagcga
     1681 gtttaaacca attgtcgtag atcttcgtca gcagagcttc accattgaag ggctggcggt
     1741 tggggttatt cgcaacggcg actggctgga attcccgggg atccgtcgac ctgcaggaat
     1801 tcccggggat ctggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg
     1861 cgaggacgtg gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg
     1921 ggacggggat tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct
     1981 ggatatggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta
     2041 cggtgggtag ggggcgcgac cggacccgca tcccccgtct gggttttccc ctcccgtccc
     2101 cggttcgtat ccacaataaa cacgagcaca tacattacaa aaacctgcgg ttgtcgtctg
     2161 attatttggt ggatccgggg gagatggggg aggctaactg aaacacggaa ggagacaata
     2221 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     2281 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     2341 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     2401 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
     2461 tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
     2521 attattttgg gcgttgcgtg gggtcaggtc cacgacccaa gcttctagat cgataccgtc
     2581 gactaaagcc aaatagaaaa ttattcagtt cctggcttaa gtttttaaaa gtgatattat
     2641 ttatttggtt gtaaccaacc aaaagaatgt aaataactaa tacataatta tgttagtttt
     2701 aagttagcaa caaattgatt ttagctatat tagctacttg gttaataaat agaatatatt
     2761 tatttaaaga taattgcgtt tttattgtca gggagtgagt ttgcttaaaa actcgtttag
     2821 atccactagc ttaattaagc atgcctaggc tagataactt cgtatagcat acattatacg
     2881 aagttatagc ttctgatgga attagaactt ggcaaaacaa tactgagaat gaagtgtatg
     2941 tggaacagag gctgctgatc tcgttcttca ggctatgaaa ctgacacatt tggaaaccac
     3001 agtacttaga accacaaagt gggaatcaag agaaaaacaa tgatcccacg agagatcgaa
     3061 ttctagtatg tatgtaagtt aataaaaccc atttttgcgg aaagtagata aaaaaaacat
     3121 tttttttttt tactgcactg gatatcattg aacttatctg atcagtttta aatttacttc
     3181 gatccaaggg tatttgatgt accaggttct ttcgattacc tctcactcaa aatgacattc
     3241 cactcaaagt cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc cgtctctttc
     3301 gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg
     3361 ccttcagttg cattttgtca gcggtttcgt gacgaagctc caagcggttt acgccatcaa
     3421 ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt atttttgttt gttttttgag
     3481 tgattggggt ggtgattggt tttgggtggg taagcagggg aaagtgtgaa aaatcccggc
     3541 aatgggccaa gaggatcagg agctattaat tcgcggaggc agcaaacacc catctgccga
     3601 gcatctgaac aatgtgagta gtacatgtgc atacatctta agttcacttg atctatagga
     3661 actgcgattg caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa
     3721 accgcaattg cacaaacaaa tagtgacacg aaacagatta ttctggtagc tgttctcgct
     3781 atataagaca atttttgaga tcatatcatg atcaagacat ctaaaggcat tcattttcga
     3841 ctatattctt ttttacaaaa aatataacaa ccagatattt taagctgatc ctagatgcac
     3901 aaaaaataaa taaaagtata aacctacttc gtaggatact tcggggtact ttttgttcgg
     3961 ggttagatga gcataacgct tgtagttgat atttgagatc ccctatcatt gcagggtgac
     4021 agcggagcgg cttcgcagag ctgcattaac cagggcttcg ggcaggccaa aaactacggc
     4081 acgctccggc cacccagtcc gccggaggac tccggttcag ggagcggcca actagccgag
     4141 aacctcacct atgcctggca caatatggac atctttgggg cggtcaatca gccgggctcc
     4201 ggatggcggc agctggtcaa ccggacacgc ggactattct gcaacgagcg acacataccg
     4261 gcgcccagga aacatttgct caagaacggt gagtttctat tcgcagtcgg ctgatctgtg
     4321 tgaaatctta ataaagggtc caattaccaa tttgaaactc agtttgcggc gtggcctatc
     4381 cgggcgaact tttggccgtg atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg
     4441 cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc atccgggatg cgactgctca
     4501 atggccaacc tgtggacgcc aaggagatgc aggccaggtg cgcctatgtc cagcaggatg
     4561 acctctttat cggctcccta acggccaggg aacacctgat tttccaagcc atggtgcgga
     4621 tgccacgaca tctgacctat cggcagcgag tggcccgcgt ggatcaggtg atccaggagc
     4681 tttcgctcag caaatgtcag cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt
     4741 ccggcggaga aaggaagcgt ctggcattcg cctccgaggc tctaaccgat ccgccgcttc
     4801 tgatctgcga tgagcccacc tccggactgg actcctttac cgcccacagc gtcgtccagg
     4861 tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct gaccattcat cagccgtctt
     4921 ccgagctgtt tgagctcttt gacaagatcc ttctgatggc cgagggcagg gtagctttct
     4981 tgggcactcc cagcgaagcc gtcgacttct tttcctagtg agttcgatgt gtttattaag
     5041 ggtatctagt attacataac atctcaactc ctatccagcg tgggtgccca gtgtcctacc
     5101 aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg acgggagatc
     5161 gagtcccgtg atcggatcgc caagatatgc gacaattttg ccattagcaa agtagcccgg
     5221 gatatggagc agttgttggc caccaaaaat ctggagaagc cactggagca gccggagaat
     5281 gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg gcgatcctgg
     5341 ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac aacggtgagt
     5401 ggttccagtg gaaacaaatg atataacgct tacaattctt ggaaacaaat tcgctagatt
     5461 ttagatagaa ttgcctgatt ccacaccctt cttagttttt ttcaatgaga tgtatagttt
     5521 atagttttgc agaagataaa taaatttcat ttaactcgcg aatattaatg agatgcgagt
     5581 aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg ggccaacaac
     5641 tcacgcaagt gggtgtgatg aatatcaacg gagccatctt cctcttcctg accaacatga
     5701 cctttcaaaa cgtctttgcc acgataaatg taagtcatgt ttagaataca tttgcatttc
     5761 aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc agagctgcca
     5821 gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt tctgggcaaa
     5881 acgattgccg aattgccgct ttttctcaca gtgccactgg tcttcacggc gattgcctat
     5941 ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc gctggtcact
     6001 ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag ctcctcgacc
     6061 tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt tggcggcttc
     6121 ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc
     6181 cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt
     6241 agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt
     6301 aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct catcgtgagc
     6361 ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca
     6421 tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt gtttactgtt
     6481 tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc
     6541 tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc
     6601 taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa
     6661 aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc
     6721 acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa
     6781 cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt
     6841 tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa taaatatttg
     6901 tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc tatatttatt
     6961 ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga gtgattagcg
     7021 ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg catctcaaaa
     7081 aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat aataataaga
     7141 atacatttaa tttagaaaat gcttggattt cactggaact agaattcata acttcgtata
     7201 gcatacatta tacgaagtta tggatctggc cgcgctcttc cgctgaagtt cctatacttt
     7261 ctagagaata ggaacttcct cgacggtagc ggtccggttg ttttcgtgct catcgcgagt
     7321 acgtatttat ttttaaaaaa taataaacgc ggcaaacaca atcacacaaa tgtgcaggca
     7381 cagtgggaca aagtgcgcta aatttggata tgtaataaat ccaaaaccaa aaagaaaaca
     7441 aaaaacggta aaatatttta ctgttgttaa aattcgatca ttcattattc gctgcatgaa
     7501 ttagctttgc gtactcgcaa attattaaaa ataaaacttt aaaaataatt tcgtctaatt
     7561 aatattatga gttaattcaa accccacgga catgctaagg gttaatcaac aatcatatcg
     7621 ctgtctcact cagactcaat acgacactca gaatactatt cctttcactc gcacttattg
     7681 caagcatacg ttaagtggat gtctcttgcc gacgggacca ccttatgtta tttcatcatg
     7741 gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg
     7801 cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt tttttaccat
     7861 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
     7921 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
     7981 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
     8041 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
     8101 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
     8161 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
     8221 cgttagttcc gcgcgattcg gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     8281 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     8341 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     8401 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     8461 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     8521 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8581 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8641 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8701 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8761 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8821 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8881 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8941 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9001 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9061 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9121 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9181 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9241 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9301 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9361 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     9841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
    10081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10261 tggaaaaacg ccttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg
    10321 tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg
    10381 gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc
    10441 atcctggaga cgcttaactt ctccgccgcc gatctg
//
