LOCUS       sequence-123582-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for FOXM1b expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Barger CJ, Zhang W, Hillman J, Stablewski AB, Higgins MJ, 
            Vanderhyden BC, Odunsi K, Karpf AR
  TITLE     Genetic determinants of FOXM1 overexpression in epithelial ovarian 
            cancer and functional contribution to cell cycle progression.
  JOURNAL   Oncotarget. 2015 Jul 16.
  PUBMED    26243836
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2015 Jul 16."
COMMENT     Sequence Label: pCW57.1-FOXM1b
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(27..35,36..71,72..74,75..110,111..113,
                     114..149,150..155,156..773))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(774..827)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gatatcttgg atcgatcctt acttagttac ccggggagca tgtcaaggtc aaaatcgtca
       61 agagcgtcag caggcagcat atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg
      121 tctaagtcaa aatcgtcaag ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc
      181 tccaggccac atatgattag ttccaggccg aaaaggaagg caggttcggc tccctgccgg
      241 tcgaacagct caattgcttg tctcagaagt gggggcatag aatcggtggt aggtgtctct
      301 ctttcctctt ttgctacttg atgctcctgt tcctccaata cgcagcccag tgtaaagtgg
      361 cccacggcgg acagagcgta cagtgcgttc tccagggaga agccttgctg acacaggaac
      421 gcgagctgat tttccagggt ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact
      481 ttagccccgt cgcgatgtga gaggagagca cagcggtatg acttggcgtt gttccgcaga
      541 aagtcttgcc atgactcgcc ttccaggggg cagaagtggg tatgatgcct gtccagcatc
      601 tcgattggca gggcatcgag cagggcccgc ttgttcttca cgtgccagta cagggtaggc
      661 tgctcaactc ccagcttttg agcgagtttc cttgtcgtca ggccttcgat accgacacca
      721 ttgagtaatt ccagagctcc gtttatgact ttgctcttgt ccagtctaga cattggacca
      781 gggttttctt caacatcacc acaagtgagg agagaacctc taccttcggc accgggcttg
      841 cgggtcatgc accaggtgcg cggtccttcg ggcacctcga c
//
