LOCUS       sequence-12359-e       11501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11501)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 11501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-QF
FEATURES             Location/Qualifiers
     source          1..11501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(25..55,56..609)
                     /label=P element 5' end
     protein_bind    complement(623..656)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    701..770
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     961..977
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3826..3842)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3826..3842)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(3879..3912)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4097..4115)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4567..4638,5040..5313,5388..6042,6104..6419,6623..6754,
                     6825..7439)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(8213..8246)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(8270..8303)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(8533..8734,8735..8765)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(9302..9321)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9421..9443
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9481..9499)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9567..9671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9672..9740,9741..10532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9890..9909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10703..11291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11192..11211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctggact acgtgggtct ggcccatgat gaaataacat aaggtggtcc cgtcgatagc
       61 cgaagcttac cgaagtatac acttaaattc agtgcacgtt tgcttgttga gaggaaaggt
      121 tgtgtgcgga caattttttt ttgaaaacat taacccttac gtggaataaa aaaaaatgaa
      181 atattgcaaa ttttgctgca aagctgtgac tggagtaaaa ttaattcacg tgccgaagtg
      241 tgctattaag agaaaattgt gggagcagag ccttgggtgc agccttggtg aaaactccca
      301 aatttgtgat acccacttta atgattcgca gtggaaggct gcacctgcaa aaggtcagac
      361 atttaaaagg aggcgactca acgcagatgc cgtacctagt aaagtgatag agcctgaacc
      421 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
      481 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattgttta
      541 atgaaaataa gagcttgagg gaaaaaattc gtactttgga gtacgaaatg cgtcgtttag
      601 agcagcagcc gaattcggta ccgaagttcc tatactttct agagaatagg aacttcggcg
      661 cgccagtacc gggccccccc tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
      721 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
      781 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
      841 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
      901 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
      961 cgaggtcgac ggtatcgata agcttaccga agtatacact taaattcagt gcacgtttgc
     1021 ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt
     1081 ggatccaaaa tgccgcctaa acgcaagaca ctcaatgccg ctgccgaagc caatgcccac
     1141 gctgatggcc atgctgatgg caatgctgat ggtcatgtcg ctaacactgc agcaagcagc
     1201 aacaacgccc gttttgcgga cttgaccaac attgacacac ccggcctcgg ccctaccacg
     1261 acgacgttac ttgtcgagcc cgctcgttcg aaacgccaga gagtctcgag ggcctgtgat
     1321 cagtgtcgag ctgcacgtga aaagtgtgat ggaatccagc cggcttgctt cccctgtgtg
     1381 tcgcagggcc ggtcgtgtac ctaccaggcc agtcccaaga agcgaggagt ccagacgggc
     1441 tacatccgca ctctcgaact ggctctggct tggatgttcg agaacgttgc ccgcagcgag
     1501 gacgccctcc acaatctttt ggtccgtgat gctggccagg gcagcgctct cctggtcggc
     1561 aaagactcgc ctgctgcaga acgcctgcat gcaagatggg cgacgagtcg agtcaacaaa
     1621 agcatcaccc gtcttctctc aggtcaggcc gcacaagatc catctgaaga cggccaatcc
     1681 ccgtccgaag acataaatgt ccaagatgcc ggggcaaaga catccgactt ccctcatgcg
     1741 cctcacttga ctttctcggc gcctaagtct agtacagctg agacacgcac tctaccaggc
     1801 ccggtccgac cacctatttc ggcaaacacc ctggaaaaca accttcagcc agatggtacc
     1861 gggataggga agctaccacc caatcattgg cgcctgctgg atatctactt ttcctacacg
     1921 cattcttggc tccctatcct cgagaagaaa gacatgtacc aagcattgta ccagtactct
     1981 gaacaaggct cgttgcttcc ctctgcgaat gtcgagtctg gcgttcatgc cgagctctgg
     2041 agcgcgctcg ccctggcgtc cttccaggct gctgctactg ctgcatcgag tgctacgggt
     2101 ccagcttcag ctgctcatgg ccatgacaat gccatcaatc cttcacctgc agacatatct
     2161 gacacagccc gaaagctcat acctttggaa agcgggccgt tccaggttca gcactgcaga
     2221 gcgttgctgc ttctttgtct cgtaagcctt gggcgggatg attgggagtc tgcttggttg
     2281 ctggttggct ttgcggtccg cgtcctactt gttgttcgca cccagttgcc tcctgatgat
     2341 gaccggccac gaccaagaat gcgtgcgctg ctcgtcgcgt gcttcatcgt ggataccatt
     2401 gtgtctatga gacacaacgt gccggcccat ctcaagccag acgacattgc ggatctgccg
     2461 ttacctgaag acggtcaaga tcaatgggag ccgtggacac catgtgaggg cttaggcggt
     2521 gaacacacca tgctgcaaat gttgaggaac ccggcatacc ctttaagcac attcaaccac
     2581 ctatatggcg tgaccaagct ggttgctttg gagcttctgc caagaatacg aacatcttca
     2641 cagaacgctc ccttggagtt caggtcgcgg ttgcagcagg taatcggcca caattctccc
     2701 ttcagcgtct ttgtcctttc ccaggataca gcatcggctt ttgtgcctac tgcatacctt
     2761 acccgtaccg tttatttatg ggcagctgcc ttttctgagc ctctcaacga acactactcg
     2821 catcttctga tcgaaactct tgatcagtat cagaagcggt ttggtacata tgcaatccca
     2881 cctctgatcc cttctcttct agactccctt cttgctttaa agaaacaatc acattcttca
     2941 gagcggcatc gaaggcactt ggaagagctt ttccccgcct actcctccat ttggcctcgg
     3001 ggaggccgac acagcaatac tggcctccaa cccatacgac aacttgagct tccgccgact
     3061 gcgactgcca ctgcaagtat catgccccat gtcatggaac agcccctgtc aacgtcaata
     3121 aatccggtca atgatcggtt taatggaata ccgaatccta ccccctacaa tagcgatgcg
     3181 gccctagacg caattactca aaccaatgac tacggatcag tcaataccca tggcattctc
     3241 agtacatatc caccacctgc cacccacttg aacgaggcct cggtggccct tgcgcctgga
     3301 ggtgctcctc ccagaccacc cccgccatac gttgatagta caacgaacca tcctccttac
     3361 catagtaacc tcgtccccat ggccaatttc ggatattcga ctgtagacta tgatgcgatg
     3421 gtggatgatc tggcatcgat cgagtacacg gatgcggttg acgttgatcc gcagttcatg
     3481 acgaacctcg ggtttgtccc agggtgtaat tttagcgata tcaacacgta tgagcaatag
     3541 taagcactag tgcagatctt atcgataccg tcgactaaag ccaaatagaa aattattcag
     3601 ttcctggctt aagtttttaa aagtgatatt atttatttgg ttgtaaccaa ccaaaagaat
     3661 gtaaataact aatacataat tatgttagtt ttaagttagc aacaaattga ttttagctat
     3721 attagctact tggttaataa atagaatata tttatttaaa gataattgcg tttttattgt
     3781 cagggagtga gtttgcttaa aaactcgttt agatccgcgg ccgcggatcc actagttcta
     3841 gagcggccgc tcgagttaat taagcatgcc taggctagat aacttcgtat agcatacatt
     3901 atacgaagtt atagcttctg atggaattag aacttggcaa aacaatactg agaatgaagt
     3961 gtatgtggaa cagaggctgc tgatctcgtt cttcaggcta tgaaactgac acatttggaa
     4021 accacagtac ttagaaccac aaagtgggaa tcaagagaaa aacaatgatc ccacgagaga
     4081 tcgaattcta gtatgtatgt aagttaataa aacccatttt tgcggaaagt agataaaaaa
     4141 aacatttttt ttttttactg cactggatat cattgaactt atctgatcag ttttaaattt
     4201 acttcgatcc aagggtattt gatgtaccag gttctttcga ttacctctca ctcaaaatga
     4261 cattccactc aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat atcgccgtct
     4321 ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg tagcgttacg tagcgtcaat
     4381 gtccgccttc agttgcattt tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc
     4441 atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt
     4501 ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc
     4561 ccggcaatgg gccaagagga tcaggagcta ttaattcgcg gaggcagcaa acacccatct
     4621 gccgagcatc tgaacaatgt gagtagtaca tgtgcataca tcttaagttc acttgatcta
     4681 taggaactgc gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat
     4741 cccaaaccgc aattgcacaa acaaatagtg acacgaaaca gattattctg gtagctgttc
     4801 tcgctatata agacaatttt tgagatcata tcatgatcaa gacatctaaa ggcattcatt
     4861 ttcgactata ttctttttta caaaaaatat aacaaccaga tattttaagc tgatcctaga
     4921 tgcacaaaaa ataaataaaa gtataaacct acttcgtagg atacttcggg gtactttttg
     4981 ttcggggtta gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg
     5041 gtgacagcgg agcggcttcg cagagctgca ttaaccaggg cttcgggcag gccaaaaact
     5101 acggcacgct ccggccaccc agtccgccgg aggactccgg ttcagggagc ggccaactag
     5161 ccgagaacct cacctatgcc tggcacaata tggacatctt tggggcggtc aatcagccgg
     5221 gctccggatg gcggcagctg gtcaaccgga cacgcggact attctgcaac gagcgacaca
     5281 taccggcgcc caggaaacat ttgctcaaga acggtgagtt tctattcgca gtcggctgat
     5341 ctgtgtgaaa tcttaataaa gggtccaatt accaatttga aactcagttt gcggcgtggc
     5401 ctatccgggc gaacttttgg ccgtgatggg cagttccggt gccggaaaga cgaccctgct
     5461 gaatgccctt gcctttcgat cgccgcaggg catccaagta tcgccatccg ggatgcgact
     5521 gctcaatggc caacctgtgg acgccaagga gatgcaggcc aggtgcgcct atgtccagca
     5581 ggatgacctc tttatcggct ccctaacggc cagggaacac ctgattttcc aagccatggt
     5641 gcggatgcca cgacatctga cctatcggca gcgagtggcc cgcgtggatc aggtgatcca
     5701 ggagctttcg ctcagcaaat gtcagcacac gatcatcggt gtgcccggca gggtgaaagg
     5761 tctgtccggc ggagaaagga agcgtctggc attcgcctcc gaggctctaa ccgatccgcc
     5821 gcttctgatc tgcgatgagc ccacctccgg actggactcc tttaccgccc acagcgtcgt
     5881 ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc atcctgacca ttcatcagcc
     5941 gtcttccgag ctgtttgagc tctttgacaa gatccttctg atggccgagg gcagggtagc
     6001 tttcttgggc actcccagcg aagccgtcga cttcttttcc tagtgagttc gatgtgttta
     6061 ttaagggtat ctagtattac ataacatctc aactcctatc cagcgtgggt gcccagtgtc
     6121 ctaccaacta caatccggcg gacttttacg tacaggtgtt ggccgttgtg cccggacggg
     6181 agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa ttttgccatt agcaaagtag
     6241 cccgggatat ggagcagttg ttggccacca aaaatctgga gaagccactg gagcagccgg
     6301 agaatgggta cacctacaag gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat
     6361 cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg
     6421 tgagtggttc cagtggaaac aaatgatata acgcttacaa ttcttggaaa caaattcgct
     6481 agattttaga tagaattgcc tgattccaca cccttcttag tttttttcaa tgagatgtat
     6541 agtttatagt tttgcagaag ataaataaat ttcatttaac tcgcgaatat taatgagatg
     6601 cgagtaacat tttaatttgc agatggttgc catcttgatt ggcctcatct ttttgggcca
     6661 acaactcacg caagtgggtg tgatgaatat caacggagcc atcttcctct tcctgaccaa
     6721 catgaccttt caaaacgtct ttgccacgat aaatgtaagt catgtttaga atacatttgc
     6781 atttcaataa tttactaact ttctaatgaa tcgattcgat ttaggtgttc acctcagagc
     6841 tgccagtttt tatgagggag gcccgaagtc gactttatcg ctgtgacaca tactttctgg
     6901 gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc actggtcttc acggcgattg
     6961 cctatccgat gatcggactg cgggccggag tgctgcactt cttcaactgc ctggcgctgg
     7021 tcactctggt ggccaatgtg tcaacgtcct tcggatatct aatatcctgc gccagctcct
     7081 cgacctcgat ggcgctgtct gtgggtccgc cggttatcat accattcctg ctctttggcg
     7141 gcttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg tacctctcat
     7201 ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg
     7261 aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc atcctggaga
     7321 cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc attctcatcg
     7381 tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc aaggagtagc
     7441 cgacatatat ccgaaataac tgcttgtttt ttttttttac cattattacc atcgtgttta
     7501 ctgtttattg ccccctcaaa aagctaatgt aattatattt gtgccaataa aaacaagata
     7561 tgacctatag aatacaagta tttccccttc gaacatcccc acaagtagac tttggatttg
     7621 tcttctaacc aaaagactta cacacctgca taccttacat caaaaactcg tttatcgcta
     7681 cataaaacac cgggatatat tttttatata catacttttc aaatcgcgcg ccctcttcat
     7741 aattcacctc caccacacca cgtttcgtag ttgctctttc gctgtctccc acccgctctc
     7801 cgcaacacat tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat
     7861 tcggttcgct caaatggttc cgagtggttc atttcgtctc aatagaaatt agtaataaat
     7921 atttgtatgt acaatttatt tgctccaata tatttgtata tatttccctc acagctatat
     7981 ttattctaat ttaatattat gactttttaa ggtaattttt tgtgacctgt tcggagtgat
     8041 tagcgttaca atttgaactg aaagtgacat ccagtgtttg ttccttgtgt agatgcatct
     8101 caaaaaaatg gtgggcataa tagtgttgtt tatatatatc aaaaataaca actataataa
     8161 taagaataca tttaatttag aaaatgcttg gatttcactg gaactagaat tcataacttc
     8221 gtatagcata cattatacga agttatggat ctggccgcgc tcttccgctg aagttcctat
     8281 actttctaga gaataggaac ttcctcgacg gtagcggtcc ggttgttttc gtgctcatcg
     8341 cgagtacgta tttattttta aaaaataata aacgcggcaa acacaatcac acaaatgtgc
     8401 aggcacagtg ggacaaagtg cgctaaattt ggatatgtaa taaatccaaa accaaaaaga
     8461 aaacaaaaaa cggtaaaata ttttactgtt gttaaaattc gatcattcat tattcgctgc
     8521 atgaattagc tttgcgtact cgcaaattat taaaaataaa actttaaaaa taatttcgtc
     8581 taattaatat tatgagttaa ttcaaacccc acggacatgc taagggttaa tcaacaatca
     8641 tatcgctgtc tcactcagac tcaatacgac actcagaata ctattccttt cactcgcact
     8701 tattgcaagc atacgttaag tggatgtctc ttgccgacgg gaccacctta tgttatttca
     8761 tcatggtctg gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact
     8821 tcgggcccga cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt
     8881 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat
     8941 ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc
     9001 ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac
     9061 atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata tacatacttt
     9121 tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt
     9181 tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg
     9241 actgtcgtta gttccgcgcg attcggtgcg gtatttcaca ccgcatatgg tgcactctca
     9301 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     9361 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     9421 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     9481 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     9541 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9601 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9661 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9721 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9781 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9841 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9901 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9961 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10021 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10081 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10141 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10201 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10261 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10321 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10381 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10441 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
    10861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
    11101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11281 gcctatggaa aaacgccttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
    11341 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
    11401 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
    11461 aggtcatcct ggagacgctt aacttctccg ccgccgatct g
//
